FastQCFastQC Report
Wed 1 Mar 2017
HK3FCAFXX_n01_lbstarf_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3FCAFXX_n01_lbstarf_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46223329
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1528120.3305949686142251TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG275500.048.6209147
ATGCCGT291350.045.98887648
CGTATGC301700.044.58343545
CTCGTAT298250.043.6489643
CGCTATC294900.043.3636137
TGCCGTC308300.043.31555649
TCTCGTA297850.043.3032542
GTATGCC312150.043.1562346
TCGTATG311450.043.00242244
GCCGTCT315700.042.0379850
TATCTCG313600.040.95339640
TTCGCTA327200.040.7484135
TCGCTAT328900.039.99263836
CCGTCTT340450.038.8714151
GCTATCT330600.038.7332438
ATCTCGT333200.038.7092741
CGTCTTC352800.037.6575352
CTTCGCT366150.036.47939734
ACTTCGC375200.035.95776733
CACTTCG382200.035.2990532