Basic Statistics
Measure | Value |
---|---|
Filename | HK3FCAFXX_n01_lbstarf_m.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 46223329 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG | 152812 | 0.3305949686142251 | TruSeq Adapter, Index 3 (97% over 37bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 27550 | 0.0 | 48.62091 | 47 |
ATGCCGT | 29135 | 0.0 | 45.988876 | 48 |
CGTATGC | 30170 | 0.0 | 44.583435 | 45 |
CTCGTAT | 29825 | 0.0 | 43.64896 | 43 |
CGCTATC | 29490 | 0.0 | 43.36361 | 37 |
TGCCGTC | 30830 | 0.0 | 43.315556 | 49 |
TCTCGTA | 29785 | 0.0 | 43.30325 | 42 |
GTATGCC | 31215 | 0.0 | 43.15623 | 46 |
TCGTATG | 31145 | 0.0 | 43.002422 | 44 |
GCCGTCT | 31570 | 0.0 | 42.03798 | 50 |
TATCTCG | 31360 | 0.0 | 40.953396 | 40 |
TTCGCTA | 32720 | 0.0 | 40.74841 | 35 |
TCGCTAT | 32890 | 0.0 | 39.992638 | 36 |
CCGTCTT | 34045 | 0.0 | 38.87141 | 51 |
GCTATCT | 33060 | 0.0 | 38.73324 | 38 |
ATCTCGT | 33320 | 0.0 | 38.70927 | 41 |
CGTCTTC | 35280 | 0.0 | 37.65753 | 52 |
CTTCGCT | 36615 | 0.0 | 36.479397 | 34 |
ACTTCGC | 37520 | 0.0 | 35.957767 | 33 |
CACTTCG | 38220 | 0.0 | 35.29905 | 32 |