FastQCFastQC Report
Wed 1 Mar 2017
HK3FCAFXX_n01_lbstard_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3FCAFXX_n01_lbstard_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17661711
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG56300.027.38920847
CGTATGC63800.023.84586345
ATGCCGT64650.023.74531748
TCGTATG64150.023.12545244
CTCGTAT58700.022.98297943
GTATGCC67300.022.91290746
TCTCGTA62050.021.85392842
TGCCGTC71550.021.69660849
AATCTCG69700.019.55488840
GCCGTCT75600.019.48475850
CCGTCTT86600.017.28893351
ATCTCGT80350.017.00544541
GGAATCT84750.016.5306438
AGGGAAT102600.015.806890536
GCTTGAA92550.015.6195960
CAAGGGA106750.015.483170534
CGTCTTC95050.015.46175452
GGGAATC92750.015.439651537
ACAAGGG108950.015.32870533
GAATCTC89400.015.24607439
Produced by FastQC (version 0.11.2)