FastQCFastQC Report
Wed 1 Mar 2017
HK3FCAFXX_n01_lbhearta_fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3FCAFXX_n01_lbhearta_fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9589057
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1089691.1363891152174816TruSeq Adapter, Index 2 (98% over 63bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG669500.6981916991420533Illumina PCR Primer Index 2 (97% over 43bp)
CTTTCGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGA288370.30072821550648826No Hit
CGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGC278300.2902266614954943No Hit
GACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACG273990.2857319546645723No Hit
GTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAA271150.2827702452910646No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG247550.25815885753938056TruSeq Adapter, Index 2 (98% over 63bp)
CCTATCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGACGCGGAGATTGTCCCCTCGGGGGCGAGTGCGGCAA211620.22068906254285484No Hit
TTTCGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAG197460.20592222989184442No Hit
GATCAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAG166470.17360414063656102No Hit
GCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCGG127890.13337077879503687No Hit
GTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGG115520.1204706573336669No Hit
GGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGA109340.11402581088004796No Hit
GGAGTCTAACATGTGCGCGAGTCATTGGGCTCTACGAAACCTAAAGGCGTAATGAAAGTGAAGGGGTGCCTCTCG102000.10637125214710895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGA101200.058.782451
CCTATCG38100.052.9812051
AAAAAGG167050.045.3793969
CTATCGA45200.044.804372
TATCGAA53200.038.1315233
TATGCCG278750.034.3796847
ATGCCGT280650.034.1478248
TGCCGTC283700.033.84115249
GCCGTCT282900.033.80288750
CCGTCTT282400.033.77775251
GTATGCC287550.033.36405646
CGTATGC295200.032.52332345
TCGTATG293950.032.4995244
CTGCTTG286200.032.21010258
GCTTGAA289850.032.1974560
ATCGAAA63400.032.0512124
TGCTTGA289600.031.89150859
CGTCTTC301400.031.86624152
CTTCTGC296300.031.84421555
CTCGTAT289200.031.77191743
Produced by FastQC (version 0.11.2)