HK3FCAFXX_n01_bay46a_fem.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HK3FCAFXX_n01_bay46a_fem.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20713705
Sequences flagged as poor quality	0
Sequence length	75
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG	302264	1.4592464264601626	RNA PCR Primer, Index 43 (97% over 43bp)
GACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACG	49412	0.23854737720750582	No Hit
CGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGC	48258	0.23297618653929847	No Hit
GATCAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAG	42287	0.20414986116679754	No Hit
CTTTCGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGA	39935	0.19279506008220162	No Hit
ATCAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGT	26549	0.12817117941961614	No Hit
GCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCGG	26300	0.12696907675377242	No Hit
TTTCGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAG	25900	0.1250379881339432	No Hit
CCTATCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGACGCGGAGATTGTCCCCTCGGGGGCGAGTGCGGCAA	25549	0.12334345787004306	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	45025	62.749744	47
TCTCGTA	43320	61.38638	42
CTCGTAT	43960	61.330746	43
ATGCCGT	45980	61.275208	48
TGCCGTC	46420	60.434727	49
TCGTATG	46320	60.382946	44
GCCGTCT	45940	60.36015	50
GTATGCC	47010	60.21798	46
CCGTCTT	45885	60.208115	51
ATCTCGT	44470	59.605232	41
CGTATGC	47885	59.017616	45
CATCTCG	45265	58.95647	40
GCTTGAA	46775	57.893597	60
GCATCTC	46405	57.49366	39
TGCTTGA	46865	56.67079	59
CTGCTTG	46765	56.584175	58
CTTCTGC	48370	56.317074	55
AGCATCT	48010	55.794693	38
TCTGCTT	47835	55.303383	57
TTCTGCT	49255	53.497974	56