FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S8_R2_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S8_R2_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715388
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGAGAGGTCCAAAACAAAGAAAAAAGGATCACCACTTGCAAAGAT54300.7590286669611456No Hit
GTATATATAATTTCCTCATTCTGTTTTTGTTTTGTTTTCAAATCCAGAGG41530.5805241351546294No Hit
CTATAAAGGCAATGTGGTAGAGAGGTCCAAAACAAAGAAAAAAGGATCAC39000.5451587110770659No Hit
TTCTAGGACTCATCGTAGGCGTGACAGCCTTGGTTCTGCTGATGATAGGG28000.39139599769635497No Hit
ATTCCAGGGTGTGCTTAAAAAGAGCAAATACTTTTCCACATTTTTCTTTG26590.37168641352664566No Hit
CTCCAGGGAGCTGCTGCTGGAGCTGGGGGCGGTGGTCAGCAGGTGCTGCT21720.3036114667844582No Hit
ATATTAATCAAGACACAAAAACGGAAATTTCTATGAACATCCTTTTTCAA20410.28529972546366444No Hit
AATTTACACTGCAGATTAACAAAGACAAAGAAAAATGTGGAAAAGTATTT19800.2767728840852796No Hit
GTTTTATAGCTTTGGTGGTCCTGAATTTCGTCAGTGGATGTGAGCATCTC19070.266568631288196No Hit
GTCTTATAATGGAATTTGAGAGACCCCAGCCTGGGCTTACCCCCCTGTGG18360.2566439470608956No Hit
GTGTAAATTTATACCAAACTAGTTTATTGAAGTAATCTGATTGACTTCCT17960.2510525756652334No Hit
GAGTATATATAATTTCCTCATTCTGTTTTTGTTTTGTTTTCAAATCCAGA17940.2507730070954503No Hit
CTCTACCACATTGCCTTTATAGTAATGAAAGTTGATTTCTGGTGTACTAG17680.24713861568826986No Hit
GTTAGTGACCATCACTGTGGGCCCAGCACTGCGTTCCGGGCTACAGGCTA17300.24182681286239074No Hit
TAACTGTACTCTACTTTGGTTTCATATATCCAATAATTTATTCCAGGGTG17210.24056875429836677No Hit
CATCTGAGGTTTCTATTTCCTGTTCCGTCATTCACCGGCTTGTGTGACCT17040.23819242145521033No Hit
TCTCAAAGTGGTGCCCCGGGCACTCGATGCGGCCAATAGACACATTTCGT16710.233579540053789No Hit
GTCCTAGAAAGAAAAAGAGAAGCTCGAGGTAGCTGCAGAAAGCAAACTCA16570.23162256006530724No Hit
CTTTAAACACTGTTTCAGAACAGGCCTTCGGGTTTCTATTTCTATTGCCT16350.22854730579769297No Hit
GCCTAGTACACCAGAAATCAACTTTCATTACTATAAAGGCAATGTGGTAG16110.22519248296029568No Hit
CTATAGACCAGGTAGACCTTTGAACCACATCTGAGGTTTCTATTTCCTGT15980.22337528725670544No Hit
GTGGTATGGGGAAGCTGTGATCTGAGCCAGGCCTGGGGAAGTTCCTCTGG15890.22211722869268144No Hit
GTTTCAGAACAGGCCTTCGGGTTTCTATTTCTATTGCCTTGATGGCTCCA15610.21820326871571794No Hit
ATCTCAAAGTGGTGCCCCGGGCACTCGATGCGGCCAATAGACACATTTCG15060.21051513304668235No Hit
GTGCTGAGTATATATAATTTCCTCATTCTGTTTTTGTTTTGTTTTCAAAT15050.21037534876179081No Hit
ATTCTGAACACTTAACATTTTCCTCCAACCTGGTGTTGGTGCATGTACAC14880.20799901591863437No Hit
ATACAAAGGGACACTCAAACCAAAGCCAGAGACAGCGAGAAGTTGCTTCT14570.20366570308699614No Hit
GTCATGGACTTGGCTGCTTGGGCCCTGGTGCGTTTGAGTTTGCTTTCTGC14440.2018485073834059No Hit
ATCATCAGCAGAACCAAGGCTGTCACGCCTACGATGAGTCCTAGAAAGAA14080.19681627312730993No Hit
GTTCTGAAACAGTGTTTAAAGAACCTGCGCCCCACCGCTGCGTGGGGGCC13890.1941603717143704No Hit
CATATATCCAATAATTTATTCCAGGGTGTGCTTAAAAAGAGCAAATACTT13720.19178403887121392No Hit
CTTCTGAAGAAGGCCTAGTACACCAGAAATCAACTTTCATTACTATAAAG13640.1906657645920815No Hit
GTACTCTACTTTGGTTTCATATATCCAATAATTTATTCCAGGGTGTGCTT13380.18703137318490107No Hit
ATCTAAGACCACAGTGCTCAAGGGAAGTCAGAAAGGGGACTCAGACCGAT13310.18605288319066018No Hit
GGCTATGGCACTCCATCCAAACTCCAAGGGCCAGGGAAGAGACATCAGCA13230.18493460891152771No Hit
ATTTATTCCAGGGTGTGCTTAAAAAGAGCAAATACTTTTCCACATTTTTC13210.18465504034174463No Hit
GGCCATGGATCTCAAAGTGGTGCCCCGGGCACTCGATGCGGCCAATAGAC13160.18395611891728686No Hit
CCCTAGGCCTGGACACACAGCCAAGTCCCTGCAAGCCTTTGGTCCCCACC12800.17892388466119086No Hit
AGCGAAAGCAGGCCATGGATCTCAAAGTGGTGCCCCGGGCACTCGATGCG12790.1787841003762993No Hit
GAATTTGAGAGACCCCAGCCTGGGCTTACCCCCCTGTGGCATCCCAGACA12670.17710668895760062No Hit
GGTTAGGGGAAGTAGTTGGGTTTGATGAATTTGATGAAATATTAATCAAG12640.17668733610292597No Hit
GTGCTAGGCCCCAGGCTTCCTGTGTGTAGCTCGGAACCCTGCCCAGGGAC12470.1743110032597695No Hit
GTGTCATCGTGACCCAGAAAAAGAAGAGGCCCTTCTGCCTACAAGGAGAA12460.17417121897487797No Hit
ACTTAAGGCTCCGTTAAAATTAAAAGGAGAGACACTCTCCATTGTTTCAT12220.17081639613748062No Hit
GCTCAAGGGAAGTCAGAAAGGGGACTCAGACCGATCATCTGGGTGTCTGC12060.16857984757921574No Hit
CCCCTGTGGCATCCCAGACATCTTCGGTCATGGACTTGGCTGCTTGGGCC11950.16704222044540865No Hit
GCCCTATCATCAGCAGAACCAAGGCTGTCACGCCTACGATGAGTCCTAGA11880.16606373045116776No Hit
CCTTTGAACCACATCTGAGGTTTCTATTTCCTGTTCCGTCATTCACCGGC11780.1646658876022522No Hit
ACATGATACATCATGTCAAGCAGCTAATTATTAGCACCGAAGACCAGCTA11650.162848691898662No Hit
CTATAAAACAGATTAAGTTATACCTAAGGCAAGATTAAGTTTGGGGACTG11590.16200998618931267No Hit
TCGTAGGCGTGACAGCCTTGGTTCTGCTGATGATAGGGCTGGTGAACTGT11350.15865516335191532No Hit
GTGGTGCCCCGGGCACTCGATGCGGCCAATAGACACATTTCGTTTCGTAT11230.15697775193321664No Hit
GTTTTAACTTTGGATAAATGGATTTCTCCCATTTTATTCTTCCTTTTCAG11220.1568379676483251No Hit
GTCTGGAGCCATCAAGGCAATAGAAATAGAAACCCGAAGGCCTGTTCTGA11140.15571969336919267No Hit
GCCCAGGGACTGCCCACTGAGCAGTCGTTCAGAGAGGGAGCCAAGAATCT11110.155300340514518No Hit
GCACAGGGGAGCATAGCTAATGGAAATTTTGGAGCCATTCTGAACACTTA11040.1543218505202771No Hit
GTTCTGCGCCCTGGGCGCCTCGGGCGTCATCAGCCGGCAGGTGAGGCACA10990.15362292909581934No Hit
GTGTTTGAACAGTGGTATGGGGAAGCTGTGATCTGAGCCAGGCCTGGGGA10890.15222508624690378No Hit
GTTCACCAGCCCTATCATCAGCAGAACCAAGGCTGTCACGCCTACGATGA10740.15012832197353046No Hit
GAAGGAAGCCAGGTGGGTTCCCGCCTCTCACCTGAGCCGCCGGAGCCGGC10700.14956918483396422No Hit
AAACAATGGAGAGTGTCTCTCCTTTTAATTTTAACGGAGCCTTAAGTTGA10650.14887026340950646No Hit
CATTTCGTTTCGTATGAAGTTTTTGGCTTGTTGTCTTATAATGGAATTTG10640.1487304791246149No Hit
GCCTTAAGTTGACTAGCTGGTCTTCGGTGCTAATAATTAGCTGCTTGACA10430.14579500914189222No Hit
AGGTATAACTTAATCTGTTTTATAGCTTTGGTGGTCCTGAATTTCGTCAG10370.1449563034325429No Hit
GGTATGGGGAAGCTGTGATCTGAGCCAGGCCTGGGGAAGTTCCTCTGGCA10360.14481651914765134No Hit
CCTTGTAGGCAGAAGGGCCTCTTCTTTTTCTGGGTCACGATGACACAGTT10300.14397781343830202No Hit
CTCCCGAACATACCTGACACACTTCTCACTTCGCTAAGAGTTTTGCCCTA10260.14341867629873578No Hit
TTCCTGGGATGGCCTCCCAAGACTCCTGCCCAGCCGTGTCTGGGTGAAAC10070.14076277488579625No Hit
CGATGACACAGTTCACCAGCCCTATCATCAGCAGAACCAAGGCTGTCACG10070.14076277488579625No Hit
TCTATATACTAAGTTTTAACTTTGGATAAATGGATTTCTCCCATTTTATT10040.14034342203112157No Hit
AAGCAGGGCTGCACAGGGGAGCATAGCTAATGGAAATTTTGGAGCCATTC9900.1383864420426398No Hit
AGCCTGGGCTTACCCCCCTGTGGCATCCCAGACATCTTCGGTCATGGACT9890.13824665775774825No Hit
GGTTGGTAGGGTGCGGAGGTCCACGAATATTGTGCTGAGGGACATTGGGC9830.1374079520483989No Hit
TGGTTAGGGGAAGTAGTTGGGTTTGATGAATTTGATGAAATATTAATCAA9800.13698859919372425No Hit
GCATGTAACTGTACTCTACTTTGGTTTCATATATCCAATAATTTATTCCA9750.13628967776926648No Hit
GGGGAGCATAGCTAATGGAAATTTTGGAGCCATTCTGAACACTTAACATT9740.13614989348437492No Hit
GTATCATGTTACCCCTTTGTCTGGAGCCATCAAGGCAATAGAAATAGAAA9740.13614989348437492No Hit
GTATAACTTAATCTGTTTTATAGCTTTGGTGGTCCTGAATTTCGTCAGTG9610.1343326977807847No Hit
TCCCCGGACCCACTGGCCTTCTCGGCGCTTGGTGCCGGCGTCGGGGTCCT9590.1340531292110016No Hit
GTACACACCTGATTGCCAGAGTTGTTTCTTTGGATCTGCTCTCTCGGCTG9560.13363377635632692No Hit
AAAGCAGGCCATGGATCTCAAAGTGGTGCCCCGGGCACTCGATGCGGCCA9480.13251550207719448No Hit
GATTACTTCAATAAACTAGTTTGGTATAAATTTACACTGCAGATTAACAA9430.1318165806527367No Hit
GGTATAACTTAATCTGTTTTATAGCTTTGGTGGTCCTGAATTTCGTCAGT9320.13027895351892957No Hit
TCTCAGTGCTGAGTATATATAATTTCCTCATTCTGTTTTTGTTTTGTTTT9250.12930046352468869No Hit
CTTCTTTTTCTGGGTCACGATGACACAGTTCACCAGCCCTATCATCAGCA9220.12888111067001404No Hit
ACCCCAGCCTGGGCTTACCCCCCTGTGGCATCCCAGACATCTTCGGTCAT9200.12860154210023092No Hit
GCCATAGCCTGTAGCCCGGAACGCAGTGCTGGGCCCACAGTGATGGTCAC9180.1283219735304478No Hit
GATTAGCAAAAATCTGAAACCCTAAAGAGATGCTCACATCCACTGACGAA9140.1277628363908816No Hit
CCTCTCACCTGAGCCGCCGGAGCCGGCCCGGGCCTCCCCGGACCCACTGG9120.12748326782109848No Hit
GTGTTTAAAGAACCTGCGCCCCACCGCTGCGTGGGGGCCAGATTTCTGGG9120.12748326782109848No Hit
GGCTTCTCCTTGTAGGCAGAAGGGCCTCTTCTTTTTCTGGGTCACGATGA9100.12720369925131536No Hit
ACATGAAACAATGGAGAGTGTCTCTCCTTTTAATTTTAACGGAGCCTTAA9000.1258058564023998No Hit
ATTTTATTCTTCCTTTTCAGAATTGCCCCTTGTTTTTGTTTCTTATTTTA8940.12496715069305049No Hit
TTGTTAATCTGCAGTGTAAATTTATACCAAACTAGTTTATTGAAGTAATC8940.12496715069305049No Hit
GTATAAATTTACACTGCAGATTAACAAAGACAAAGAAAAATGTGGAAAAG8900.12440801355348427No Hit
AACTTAATCTTGCCTTAGGTATAACTTAATCTGTTTTATAGCTTTGGTGG8890.12426822926859271No Hit
GCTTGGGCCCTGGTGCGTTTGAGTTTGCTTTCTGCAGCTACCTCGAGCTT8850.12370909212902649No Hit
GCAGGAGAGGAGGCAGCAGGGAAATCCTGCTCGCATCCCCTAGGCCTGGA8840.12356930784413493No Hit
GCCAATAGACACATTTCGTTTCGTATGAAGTTTTTGGCTTGTTGTCTTAT8820.12328973927435181No Hit
ATATATAATTTCCTCATTCTGTTTTTGTTTTGTTTTCAAATCCAGAGGTA8710.12175211214054471No Hit
TGATAGGGCTGGTGAACTGTGTCATCGTGACCCAGAAAAAGAAGAGGCCC8640.12077362214630383No Hit
GCCTTTATAGTAATGAAAGTTGATTTCTGGTGTACTAGGCCTTCTTCAGA8630.12063383786141227No Hit
CCCTAAAGAGATGCTCACATCCACTGACGAAATTCAGGACCACCAAAGCT8630.12063383786141227No Hit
GCTTACCTGCCGTTCAGGCAGAAAATGAGAGCCAGAATCTAAGACCACAG8600.1202144850067376No Hit
CCCCGGACCCACTGGCCTTCTCGGCGCTTGGTGCCGGCGTCGGGGTCCTG8570.11979513215206294No Hit
GTTAATCTGCAGTGTAAATTTATACCAAACTAGTTTATTGAAGTAATCTG8570.11979513215206294No Hit
GTTGTCTTATAATGGAATTTGAGAGACCCCAGCCTGGGCTTACCCCCCTG8560.11965534786717137No Hit
GGGTATGCCAGGGAAATTCTTGGCTAATTATTTCTCACTCCCGAACATAC8520.11909621072760516No Hit
GCTCCAGACAAAGGGGTAACATGATACATCATGTCAAGCAGCTAATTATT8470.11839728930314738No Hit
ATATTGTGCTGAGGGACATTGGGCAATGGGGAGCCATGGGGGGTGTTTGA8440.11797793644847271No Hit
ATGTGGTAGAGAGGTCCAAAACAAAGAAAAAAGGATCACCACTTGCAAAG8410.11755858359379806No Hit
GTCCAAAACAAAGAAAAAAGGATCACCACTTGCAAAGATTAGCAAAAATC8390.11727901502401494No Hit
CTTAAAAAGAGCAAATACTTTTCCACATTTTTCTTTGTCTTTGTTAATCT8360.11685966216934028No Hit
TCTATAGACCAGGTAGACCTTTGAACCACATCTGAGGTTTCTATTTCCTG8340.11658009359955716No Hit
GTACAAAGACTTGGGCAAACCTCCTTGACCCTCCCTTCACAAATCCACCA8340.11658009359955716No Hit
CATCGTAGGCGTGACAGCCTTGGTTCTGCTGATGATAGGGCTGGTGAACT8310.1161607407448825No Hit
GTTTTTGGCTTGTTGTCTTATAATGGAATTTGAGAGACCCCAGCCTGGGC8230.11504246646575006No Hit
GAATACTTGCCAGGAGAGAAAAGAGCCTGAAAACCTGCTGAAGGCTTCTG8210.11476289789596693No Hit
CATGATACATCATGTCAAGCAGCTAATTATTAGCACCGAAGACCAGCTAG8200.11462311361107538No Hit
GGTCAGCAGGTGCTGCTGTTCTGCGCCCTGGGCGCCTCGGGCGTCATCAG8180.11434354504129228No Hit
GTACACCAGAAATCAACTTTCATTACTATAAAGGCAATGTGGTAGAGAGG8140.11378440790172607No Hit
TTCTGAACCTGTTTGCTCATCAGTGACGGGGCTGCTGATGTCTCTTCCCT8110.1133650550470514No Hit
CCTACGATGAGTCCTAGAAAGAAAAAGAGAAGCTCGAGGTAGCTGCAGAA8010.11196721219813584No Hit
GCCCAGGGTATGCCAGGGAAATTCTTGGCTAATTATTTCTCACTCCCGAA7920.11070915363411184No Hit
CTGCTGTTCTGCGCCCTGGGCGCCTCGGGCGTCATCAGCCGGCAGGTGAG7920.11070915363411184No Hit
ACTTTGGTTTCATATATCCAATAATTTATTCCAGGGTGTGCTTAAAAAGA7880.11015001649454562No Hit
GGTTTCATATATCCAATAATTTATTCCAGGGTGTGCTTAAAAAGAGCAAA7860.10987044792476251No Hit
CTTTAGGGTTTCAGATTTTTGCTAATCTTTGCAAGTGGTGATCCTTTTTT7810.10917152650030473No Hit
TCCCTGGCCCTTGGAGTTTGGATGGAGTGCCATAGCCTGTAGCCCGGAAC7800.10903174221541317No Hit
GAAGAAGGCCTAGTACACCAGAAATCAACTTTCATTACTATAAAGGCAAT7800.10903174221541317No Hit
CAATAAACTAGTTTGGTATAAATTTACACTGCAGATTAACAAAGACAAAG7790.10889195793052163No Hit
GTAGTTGGGTTTGATGAATTTGATGAAATATTAATCAAGACACAAAAACG7760.10847260507584695No Hit
GTCCTGGCGGGCGCCCCCCTGTCTGCGGTGCTGGCTGAGCTCTCCAGGGA7750.10833282079095538No Hit
AACTTAATCTGTTTTATAGCTTTGGTGGTCCTGAATTTCGTCAGTGGATG7750.10833282079095538No Hit
ACCTGATACAAAGGGACACTCAAACCAAAGCCAGAGACAGCGAGAAGTTG7750.10833282079095538No Hit
CTACTTTGGTTTCATATATCCAATAATTTATTCCAGGGTGTGCTTAAAAA7730.10805325222117228No Hit
TCTCTCCCCAGTCCCCAAACTTAATCTTGCCTTAGGTATAACTTAATCTG7710.10777368365138917No Hit
TCCTTTTTCAACACCTGATACAAAGGGACACTCAAACCAAAGCCAGAGAC7670.10721454651182295No Hit
ATGTAACTGTACTCTACTTTGGTTTCATATATCCAATAATTTATTCCAGG7640.10679519365714829No Hit
CTGTGGGCCCAGCACTGCGTTCCGGGCTACAGGCTATGGCACTCCATCCA7590.1060962722326905No Hit
GAAGTAATCTGATTGACTTCCTCGCCTCCAGGTTCATCTCTCCCCAGTCC7580.10595648794779897No Hit
CGCCTACGATGAGTCCTAGAAAGAAAAAGAGAAGCTCGAGGTAGCTGCAG7530.10525756652334117No Hit
AGGTAGACCTTTGAACCACATCTGAGGTTTCTATTTCCTGTTCCGTCATT7440.10399950795931719No Hit
GATTAAGTTATACCTAAGGCAAGATTAAGTTTGGGGACTGGGGAGAGATG7370.10302101796507629No Hit
TCCCAGACATCTTCGGTCATGGACTTGGCTGCTTGGGCCCTGGTGCGTTT7360.10288123368018473No Hit
AGTTTATTGAAGTAATCTGATTGACTTCCTCGCCTCCAGGTTCATCTCTC7300.1020425279708354No Hit
AATTTATTCCAGGGTGTGCTTAAAAAGAGCAAATACTTTTCCACATTTTT7260.10148339083126919No Hit
GTGCTAATAATTAGCTGCTTGACATGATGTATCATGTTACCCCTTTGTCT7220.10092425369170295No Hit
GCCAAGTCCCTGCAAGCCTTTGGTCCCCACCCAGATTTGGGGCAGAATGC7210.1007844694068114No Hit
GGGCTGCACAGGGGAGCATAGCTAATGGAAATTTTGGAGCCATTCTGAAC7210.1007844694068114No Hit
AAAGCAGGGAGCTGCTGCTGGAGCTGGGGGCGGTGGTCAGCAGGTGCTGC7200.10064468512191985No Hit
GTAGAAAACTGATATATGCCATCAAGAGAGGGTGGGGTAGGAACAGCACA7200.10064468512191985No Hit
AATTTATACCAAACTAGTTTATTGAAGTAATCTGATTGACTTCCTCGCCT7190.1005049008370283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)