FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S8_R2_H11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S8_R2_H11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237364
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT81250.3631505646823673No Hit
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA77010.34419969213771207No Hit
ATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGACA65630.2933362653551233No Hit
GTATTAACTCTGAATAATAACGAGTCTACGTGGATGCCCCATCCTACCAA55410.2476575112498458No Hit
TTATAATACTCTATCATCCACAAAGAAATGGTCAACCAAAATCTGCCTAT54270.24256222948076397No Hit
GTCATGAGAAGCATATCCTTTTTTTGTTGTTGTTGTTTTGTGTGTGTGTG51230.22897481142987908No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT45240.2022022344151421No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT43990.196615302650798No Hit
CTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGG43660.19514035266501115No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT42360.18932994363009326No Hit
ATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGA41230.1842793573151262No Hit
TTCTTATACATGCCCTTAAGGACCATCTGAATGTGAAGACTCGAGAATTA40840.18253623460465085No Hit
GTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGA38730.17310549378643797No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT38550.17230097561237243No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC36480.16304901661061857No Hit
GATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGG35010.1564787848557499No Hit
CTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT33360.14910403492681565No Hit
AGATGGGACCCTGCAAAAGAAGACGATGAAGAGATCTCTGGGAAATGAAT33160.1482101258445206No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT33020.14758438948691407No Hit
CCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGATT32230.14405344861184857No Hit
GGATTTATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGA31770.14199745772256997No Hit
ACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGA30100.13453331688540623No Hit
AATATATTCTTACCAATTGCCATGGAATCTTATGAGTTAAGTCAGAGGTT29610.13234323963378333No Hit
GGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGC29270.13082359419388173No Hit
CTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCAT27790.1242086669848983No Hit
TCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCAC27670.12367232153552127No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA27660.12362762608140651No Hit
GTGCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAAGAGTA27580.12327006244848848No Hit
GTACTAGTTTTGCTTCTTTGACCAAGAGCTGACATAAGAAGGTGATGTAT26940.1204095533851443No Hit
AACTAGTACAGGGCAGCATTAACTAAGAAGAGATAAAGACTGAGTCTTCA26920.1203201624769148No Hit
GAACTCAAACTCAGTGATTATGATGGACGACTAATCCAGAATAGCATAAC26510.11848764885820993No Hit
GTCTTTATCTCTTCTTAGTTAATGCTGCCCTGTACTAGTTTTGCTTCTTT26460.11826417158763616No Hit
TCCCAGCACTGGGATGCTCTTCTAGGTATTTATTTCTTCTCTCATCAAAA25730.1150014034372592No Hit
AATGGAGACTGGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGCATCTG25140.11236437164448879No Hit
TCCTAATCCAGGTTTTTGAAGACTTTTGAAGGTTTTGAATCCAGTATTTT25140.11236437164448879No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA25040.11191741710334126No Hit
GATAAAGACTGAGTCTTCAAAATACTGGATTCAAAACCTTCAAAAGTCTT24660.11021898984698066No Hit
GAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTT24650.1101742943928659No Hit
GACTTAAAGCTTGTTGATAAGGAATTAGAATTCGTTTCACCTTCAAGTCG24580.10986142621406261No Hit
CCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAG24530.10963794894348887No Hit
CTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTG24290.10856525804473478No Hit
ATATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACC24270.10847586713650528No Hit
CAATAGAGAAGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATAC23540.10521309898612831No Hit
TCTTATGTCAGCTCTTGGTCAAAGAAGCAAAACTAGTACAGGGCAGCATT23500.10503431716966931No Hit
GGATAGGAACAGTGAAGGTGAGAAAACTGAAAGAGAAACTCAAAGATACA23170.10355936718388246No Hit
TATCATGATTTGGCATTCCAACCTGAATGATGCCACATATCAGAGAACAA23120.1033358899133087No Hit
CCTTATTTCTTCTTTGTCATAAAGGATAAGTTCTCTCATCCACTTTCTGT22970.10266545810158739No Hit
GAGTTAATACAGGTCTCTGTGCCACAAAAACTTTAACCTTCTCAACTCCA22830.10203972174398088No Hit
GCTCTATACAATTCACCTTGATGGAGGCCCCAAAGGAGACAGACATGAAA22570.10087763993699729No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)