FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S8_R2_G12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S8_R2_G12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777296
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGAAGAGAGTGGCATTCAGAAAAGCGAGGAAACAAACCAACAGGTAAC97800.550274124287682No Hit
GTATATGCTACTGCAAATTCCCACCATCGTATCCTTCTAGCACAAGTGGT71820.4040970102897885No Hit
CACTAGAAGAGAGTGGCATTCAGAAAAGCGAGGAAACAAACCAACAGGTA63680.3582970985136972No Hit
GCTCTAATCTTGAATTTGAACCCATACCATAGGTTAATGCCGAATTAATG46480.2615208721563544No Hit
CTTTCACACTGCTCTAATCTTGAATTTGAACCCATACCATAGGTTAATGC42860.24115285242300666No Hit
GTTTAATTATGACTACACACTAGAAGAGAGTGGCATTCAGAAAAGCGAGG42370.23839585527678No Hit
GTGTTACACAACCATAATTTTGCAATCATAAAGGTCATTCTAAGTAATCA38090.21431432918320864No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG37500.21099467955816026No Hit
CCATAGGTTAATGCCGAATTAATGCAAAGCAGAGTTCCATCTATTCAGAG34400.19355245271468569No Hit
GTTACATTCTGTATATGCTACTGCAAATTCCCACCATCGTATCCTTCTAG34210.19248341300492433No Hit
GTTATATTTCCCTGTGTTACACAACCATAATTTTGCAATCATAAAGGTCA32590.1833684428480118No Hit
GTCATAAACTGTTTTCTAGATAACTTAAGCTTATTGCTCTTTAAGCCCCA31840.1791485492568486No Hit
TTCTTATACATGCCCTTAAGGACCATCTGAATGTGAAGACTCGAGAATTA31190.17549130814450717No Hit
CTGTATATGCTACTGCAAATTCCCACCATCGTATCCTTCTAGCACAAGTG29800.1676704386888847No Hit
GTACTAAAGTATCTATGGGTGGCTAGCCCCAACAGCCCAATATTACTGTT29270.16468838055112936No Hit
ATATTACTGTTATATTTCCCTGTGTTACACAACCATAATTTTGCAATCAT27500.15472943167598419No Hit
CATAAAGGTCATTCTAAGTAATCAAGAAAAGACTGTTACATTCTGTATAT26890.15129725155517146No Hit
ATATTTCCCTGTGTTACACAACCATAATTTTGCAATCATAAAGGTCATTC26410.148596519656827No Hit
GCCCAATATTACTGTTATATTTCCCTGTGTTACACAACCATAATTTTGCA24210.13621816512274826No Hit
GTCTATAATTTTCCCATAATCTTATTATGCAAATGAAACTGATGAAAACT24160.13593683888333738No Hit
CTGTTATATTTCCCTGTGTTACACAACCATAATTTTGCAATCATAAAGGT23320.1312105580612346No Hit
CACCTATTTCAGGCTGGGATTTTGTGGCATCTGCTTGGTTATCTGTTTAA21140.11894473402292022No Hit
AGCATATACAGAATGTAACAGTCTTTTCTTGATTACTTAGAATGACCTTT20050.11281182200376302No Hit
GGAATAATGAATGACAATGACTAAACAAGGAGAGACAGGAAAAAACATTC19690.11078627308000469No Hit
ATCTATGGGTGGCTAGCCCCAACAGCCCAATATTACTGTTATATTTCCCT19330.10876072415624634No Hit
TTCTAGCACAAGTGGTTAAGATAAAAAGTTCTGGGGCTTAAAGAGCAATA18780.10566613552272666No Hit
GGTTAATGCCGAATTAATGCAAAGCAGAGTTCCATCTATTCAGAGCCCCA18660.10499095254814055No Hit
GTATTAACACTGTAAAGCGACAGATTACAGTAACTAGACCTCTCCTGGTG18370.10335926035955745No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG18330.10313419936802874No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)