FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S8_R2_G09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S8_R2_G09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802405
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT48470.26891847281826226No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG33340.18497507496927718No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA31770.17626449105500705No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA31270.17349041974472995No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA30060.16677716717385938No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC26450.1467483723136587No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA24830.13776038126836088No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC23490.13032587015681826No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT19390.10757848541254603No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG19160.10630241260981856No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA18550.10291804561128048No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)