FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S8_R2_G08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S8_R2_G08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520977
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA66610.43794219110479643No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA57310.3767972822731705No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG50290.33064273818736245No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC45120.2966514286540822No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA41970.2759410563078863No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC38760.2548362006789057No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT36150.23767617787777195No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC35610.23412582833270984No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT33370.21939845244208162No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA32200.2117060284277803No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT31820.2092076343034773No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC31320.20592027361360493No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG30570.2009892325787964No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG29830.1961239387577853No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC27590.1813965628671571No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG27220.17896391595665154No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA26730.17574230248057662No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA26450.1739013804942481No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA26180.17212620572171702No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT26040.17120574472855277No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA25920.1704167781629834No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC25830.16982505323880637No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA25300.16634045090754165No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA23980.15766181868627863No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG23300.1531910081480522No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA22780.14977215303058494No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT22310.14668203398210494No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA21960.14438088149919426No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG21200.1393840932505883No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT20950.1377404129056521No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA20640.13570224927793123No Hit
AAATTACACTGGCCATTCACCAGAAAAAAAATGAACTCAAGCCCAACCAT20620.13557075485033632No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT20360.1338613272916027No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA19680.1293905167533763No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA19660.12925902232578138No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT19570.12866729740160435No Hit
CTTAACCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTACCT19560.12860155018780692No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA19540.12847005576021203No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA19510.1282728141188197No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT19240.1264976393462886No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA18630.12248705930464432No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC18320.12044889567692345No Hit
CTCTGATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATC18140.11926544582856939No Hit
CATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGATATGT17730.11656981006287405No Hit
CCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAACTATCCATACAAA17070.11223049395224254No Hit
TCCCAGGACTTTGGAATCATGACCTGAGCTGAAGGCAGACACTTAACAAC16980.11163876902806551No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA16570.10894313326237016No Hit
GAAGGAAAGAGAGCAAACAGGGGGAGCAACAGGCAGAGGGAGAGGGAGAA16440.10808841948300336No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC16340.10743094734502888No Hit
ATATATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAAT16190.10644473913806718No Hit
CTGTTAAGGCCCTGCAGTGGCTTTTCCAATTCAGATATTGTATTTTTTTA16140.10611600306907994No Hit
TCCATGATGTGGCCTGAGGAAAAAGTAAGATACAGTAGATACAGAGAAAT16090.1057872670000927No Hit
GGCCACATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGA16010.10526128928971314No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA15970.10499830043452334No Hit
CTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTA15880.10440657551034631No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG15850.10420933386895398No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC15780.10374910337237185No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA15690.10315737844819482No Hit
ATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTC15680.10309163123439737No Hit
CTGATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCA15460.10164519253085354No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT15410.10131645646186628No Hit
GTACTAAACCGTACACTTTAGAATGATTAAGGTGTGAGTTTTGTGTTATT15350.10092197317908161No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)