FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S8_R2_G02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S8_R2_G02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964872
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACGCCTCGCACAAGCAAGCCAAGGTCTGCCTCGTGTCCCGCATCAG90850.46237108575011504No Hit
GTATATACCCTCTACTGTGGATGGCAGAGGGGCTTTGGGAAAACATAGAC87730.44649218880415625No Hit
CTATATGACACTCTTGTTTCATTAGCATTACAACAGATTATTAGACAAAT70430.35844574099483323No Hit
CTCTAGAGGTGGCAGTCTGGGTTCTAACACACCCTCAGGGATTCTGATTC43820.22301707184997294No Hit
GATTTAAGGTGGGTGAGAAATATTGTTTGAAACTATAAGTGAAGGCTTTC41900.21324544296015213No Hit
TTATTAGACAAATCAACAGGATGAAAATTTAAAGCAACGTTATCTAACTG39180.199402302032906No Hit
CTCTAGAGCAGCCGCGGCCTTCTTGTCCTTCTTCTTCTTCTTCTCCTTCT38670.19680671310904732No Hit
CTCCAGACCTGCTGAATCACAAACTCTAGAGGTGGCAGTCTGGGTTCTAA34820.17721256142893788No Hit
GCTCTAGAGAGTGCGGCCGGCCCGGGACACGGGGACAGTGACGGTACCAA31540.16051936207549397No Hit
CCCTCAGGGATTCTGATTCATGCTAAAGTCTGAGAACCAGAGATCTACCT29520.15023879418099498No Hit
CTATATCACCGTGAATCTTGTTAGAAACGCAGACTCTCAAGCCCCACTCC28640.14576013093982712No Hit
GGCCACGACAGCGACGAGTCGGGGAGCTCCTTCTTTTGGAACCAGCTGCT27730.1411287859972558No Hit
GTTCTGGGCTGTGCCAGATCTGAAGGAGACGATCCCCAACTCTCCCAAGC27360.1392457116799466No Hit
TATCTGGCCTGGGCACGAGATTTTTAAATCTTCCCTGATGATTTAAGGTG26820.13649744105468448No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT26720.13598850205000632No Hit
AGCGAAAGCAGGGCCACGACAGCGACGAGTCGGGGAGCTCCTTCTTTTGG24620.1253007829517648No Hit
GCTATATCACCGTGAATCTTGTTAGAAACGCAGACTCTCAAGCCCCACTC24230.12331592083351996No Hit
ATATAAAACAGTGGGATTAGGGCTCAGAGTATAGGAAGTTTCTCCCTTCC23790.12107658921293601No Hit
AAAGAGGACACTCCGTCATCCATGTAAATCGTCTGCTCAGTCTCCACGAA23130.11771759178206011No Hit
GGCATTGGCTTCGAGGCCTCTGTTGAGTCTCAGATGATGAGAGCCAACCT23090.11751401618018883No Hit
GTCTCAGATGATGAGAGCCAACCTGAAGCCCTGGCTGCTCCCAAAACAGC21980.11186479322826118No Hit
ATATGACACTCTTGTTTCATTAGCATTACAACAGATTATTAGACAAATCA21950.11171211152685773No Hit
GTATAACGGACCCCAGGTATCCGGCCGCCAGAATCAACATGGCTGTCATG21600.10993082501048414No Hit
GTATAGGAAGTTTCTCCCTTCCGAGTCATTCTTTAATCTCATTCCAACAT21480.10932009820487033No Hit
GGCTTCGAGGCCTCTGTTGAGTCTCAGATGATGAGAGCCAACCTGAAGCC21430.10906562870253125No Hit
CTGTTGAGTCTCAGATGATGAGAGCCAACCTGAAGCCCTGGCTGCTCCCA20850.10611378247539788No Hit
GTATCTGGCCTGGGCACGAGATTTTTAAATCTTCCCTGATGATTTAAGGT20800.10585931297305881No Hit
CTATAAGTGAAGGCTTTCTCAGTTAGATAACGTTGCTTTAAATTTTCATC20220.10290746674592544No Hit
GGGTATATACATCCTATTCTCCAGGAAGAAAGAGGGAAGGGTAATTAGAA19710.10031187782206677No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)