FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S8_R2_E09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S8_R2_E09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84048
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTAC4810.5722920236055588No Hit
GTGTCATAGTTTGTCAACCATGATCTAAGAAATGTACTCTCCAACCCAAT4730.5627736531505806No Hit
GTCATAGTTTGTCAACCATGATCTAAGAAATGTACTCTCCAACCCAATAC4170.4961450599657339No Hit
GTAAGAAGGCCAAGGCCCCTGAAAGGCCAGGGACTCGCGTCAGTGAGCGT3800.4521225966114601No Hit
AGCAGGGGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAG3790.4509328003045879No Hit
ACCCAATACTTCCCTCAGTTCCTTGACAAAAAGGCTTCTCCCTGGCTTCA3610.4295164667808871No Hit
ATCTTAGGGGGAGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGTCCAT3570.42475728155339804No Hit
GAATTACAGCAGGAGCTGAAGCTGGAGTGGGTCGAGGCCTAGGTGCTGGC3220.3831144108128688No Hit
TTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTACA3190.37954502189225203No Hit
GATCTGGACCAAGCCAAGGAGGAGGTGTTCCGCCTACCCCAAGAGGAGGA3180.37835522558537976No Hit
CTCCAACCCAATACTTCCCTCAGTTCCTTGACAAAAAGGCTTCTCCCTGG3060.3640776699029126No Hit
GGTGGAGGCAGTCACCGGCGCAGTAAGAAGGCCAAGGCCCCTGAAAGGCC3010.35812868836855133No Hit
GAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAGCCAGATCC2990.3557490957548068No Hit
AGCGAAAGCAGGGGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGC2960.35217970683419No Hit
GTATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGG2790.3319531696173615No Hit
GAACAAGAGGGAGGAATCGGTGAAGGAGGCAGTGGGGGAGAAGGCAAGAC2610.3105368360936608No Hit
GAATTGGAACAGGGATTGGGGCCGGAATTACAGCAGGAGCTGAAGCTGGA2580.306967447173044No Hit
GTGTGTCATAGTTTGTCAACCATGATCTAAGAAATGTACTCTCCAACCCA2550.30339805825242716No Hit
CTTAACAGCTGAGCCCCAGGCACCCTGGGCCTGATCTCTTTTTACTGAAG2420.28793070626308775No Hit
GATCTAAGAAATGTACTCTCCAACCCAATACTTCCCTCAGTTCCTTGACA2410.2867409099562155No Hit
GTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAGCCAGATCCCC2360.2807919284218542No Hit
CCCCAAGAGGAGGAGGAGGGGCCAGGGGCTGGGGATGAGGTTTCCTGTGG2300.27365315058062056No Hit
GAAATGTACTCTCCAACCCAATACTTCCCTCAGTTCCTTGACAAAAAGGC2260.26889396535313154No Hit
CTCCTGTCACATCCTCCCATCATACCCGCAGCACTTCCACAACCCCACGC2130.25342661336379213No Hit
GTCCTGGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGCGCAGGC2100.24985722444317532No Hit
CTAATAAACTAAACCATTGGAATTTCAAGTCTTGACTTTCACACCCAGGG2070.24628783552255853No Hit
AAAGCAGGGGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAG2040.24271844660194172No Hit
AAAGCAGGCGGTGTGTCATAGTTTGTCAACCATGATCTAAGAAATGTACT2030.2415286502950695No Hit
AGCAGGCGGTGTGTCATAGTTTGTCAACCATGATCTAAGAAATGTACTCT1990.23676946506758045No Hit
TCTTCGTGGAGCCCGGGCTGAGACTCAAGGGGCAAACCACACTCCTGTCA1920.2284408909194746No Hit
GTGCAGGCAGGAGGAGGGGCACAGGCAGAAGAACAAGAGGGAGGAATCGG1900.22606129830573005No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA1880.22368170569198553No Hit
GGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAGCCAGATCCCCGG1860.221302113078241No Hit
CTCTGACACCTTGTGAATGGTTGGGGTAAATTACAGATTTTCCTGGAAAG1770.21059394631639064No Hit
CTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCCAGC1710.20345516847515704No Hit
TCCCCGGACCCTGGGATCAGGACCTGAGCCAAAGGCACACACTTAACAGC1680.19988577955454026No Hit
CCTGTCACATCCTCCCATCATACCCGCAGCACTTCCACAACCCCACGCTG1680.19988577955454026No Hit
GGTCTGGGTCCTGTCCATCAGGATTTTCACAGACTTGGTGTTCTTCATGA1660.19750618694079572No Hit
GGCTTCTGCTGAGCAGAGAGCCAGATCCCCGGACCCTGGGATCAGGACCT1660.19750618694079572No Hit
GGCCAGGGGCTGGGGATGAGGTTTCCTGTGGGACTGGTGGAGGCAGTCAC1650.19631639063392345No Hit
GGGATTGGGGCCGGAATTACAGCAGGAGCTGAAGCTGGAGTGGGTCGAGG1600.19036740909956215No Hit
GCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCC1600.19036740909956215No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG1590.18917761279268988No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA1580.18798781648581764No Hit
CTCCCTGGCAGGGCTGCAGCGTGGGGTTGTGGAAGTGCTGCGGGTATGAT1580.18798781648581764No Hit
CTGCTGAGCAGAGAGCCAGATCCCCGGACCCTGGGATCAGGACCTGAGCC1560.18560822387207312No Hit
GCAGGGGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAGC1540.18322863125832856No Hit
GGCAGGAGGAGGGGCACAGGCAGAAGAACAAGAGGGAGGAATCGGTGAAG1540.18322863125832856No Hit
AGCGAAAGCAGGCGGTGTGTCATAGTTTGTCAACCATGATCTAAGAAATG1490.17727964972396726No Hit
AGTGGAAGCTGCCCGCAAGGATCTGGACCAAGCCAAGGAGGAGGTGTTCC1480.176089853417095No Hit
GGTTTCCTGTGGGACTGGTGGAGGCAGTCACCGGCGCAGTAAGAAGGCCA1470.17490005711022272No Hit
GTGGGGGAGAAGGCAAGACAGGAGCAGGAAGCATGGTTATTGGACTGGGG1470.17490005711022272No Hit
GTCTATAGGAGATCCGTCTCTGAGCTGCTGAACTGGGAGCACTGGTAGAA1460.17371026080335047No Hit
GTGAAGGAGGCAGTGGGGGAGAAGGCAAGACAGGAGCAGGAAGCATGGTT1460.17371026080335047No Hit
ATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGGAG1440.17133066818960593No Hit
GGCCAAGGCCCCTGAAAGGCCAGGGACTCGCGTCAGTGAGCGTCTTCGTG1420.16895107557586142No Hit
GTCTATATGGCACCAACTTTCTTCCACCATTTTCTTTAGTTTCAGTGCCT1410.16776127926898915No Hit
GTGGAGGAGAAGGTGAGCCAGCTGAAGGAGGAGGTGCGGCTACAGTATGA1400.16657148296211688No Hit
CTGTGGGACTGGTGGAGGCAGTCACCGGCGCAGTAAGAAGGCCAAGGCCC1390.1653816866552446No Hit
CAGGGGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAGCC1380.16419189034837237No Hit
GGGATGAGGTTTCCTGTGGGACTGGTGGAGGCAGTCACCGGCGCAGTAAG1380.16419189034837237No Hit
GACTTGGTGTTCTTCATGAAGCTGACGTCTTCTGTCTTGTCGAAGAGCAG1370.1630020940415001No Hit
TTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGCCACCTCATTCAGGA1350.16062250142775555No Hit
GGCAGAAGAACAAGAGGGAGGAATCGGTGAAGGAGGCAGTGGGGGAGAAG1330.15824290881401104No Hit
ACCATGATCTAAGAAATGTACTCTCCAACCCAATACTTCCCTCAGTTCCT1320.15705311250713877No Hit
TTCTTGGCCACCTCATTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTT1310.15586331620026653No Hit
GCTGAGCAGAGAGCCAGATCCCCGGACCCTGGGATCAGGACCTGAGCCAA1310.15586331620026653No Hit
AAAGCAGGCAGAAGAACAAGTGGAAGCTGCCCGCAAGGATCTGGACCAAG1300.15467351989339426No Hit
AAGCAGGACATCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAGCGCCT1290.153483723586522No Hit
GTGCTGAAGGGGCCTTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGC1270.15110413097277745No Hit
GGCTGAGACTCAAGGGGCAAACCACACTCCTGTCACATCCTCCCATCATA1270.15110413097277745No Hit
GGGGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAGCCAG1270.15110413097277745No Hit
CCCTGGGCCTGATCTCTTTTTACTGAAGCCAGGGAGAAGCCTTTTTGTCA1260.1499143346659052No Hit
GTACTCTCCAACCCAATACTTCCCTCAGTTCCTTGACAAAAAGGCTTCTC1260.1499143346659052No Hit
GTGCTGGCCGGGGAACTCTCTCCCTGGCAGGGCTGCAGCGTGGGGTTGTG1230.14634494574528842No Hit
GGAGTGGGTCGAGGCCTAGGTGCTGGCCGGGGAACTCTCTCCCTGGCAGG1210.14396535313154388No Hit
CAGTAAGAAGGCCAAGGCCCCTGAAAGGCCAGGGACTCGCGTCAGTGAGC1180.1403959642109271No Hit
GTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAA1180.1403959642109271No Hit
CTCATGAGCAAACCGCACATCAGAGGAATTAAAAGATGACCTGATGGAGA1180.1403959642109271No Hit
TCATGAAGCTGACGTCTTCTGTCTTGTCGAAGAGCAGCTGCAGGGAGCCC1160.13801637159718258No Hit
GGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTACAGCAGG1140.13563677898343804No Hit
GTTCTGTTCCATGGAAACACTTTCCGTAGAGACCAGGGTTTCTGGTCCTG1130.13444698267656577No Hit
CTACATGGGCTTTGGGGCAGGCAGAGGCTCCCTGTCGGGATGATTTACTG1110.13206739006282125No Hit
CACTTAACAGCTGAGCCCCAGGCACCCTGGGCCTGATCTCTTTTTACTGA1110.13206739006282125No Hit
GGTATGATGGGAGGATGTGACAGGAGTGTGGTTTGCCCCTTGAGTCTCAG1110.13206739006282125No Hit
CATCATACCCGCAGCACTTCCACAACCCCACGCTGCAGCCCTGCCAGGGA1090.1296877974490767No Hit
CCACACTCCTGTCACATCCTCCCATCATACCCGCAGCACTTCCACAACCC1090.1296877974490767No Hit
GATCAGGACCTGAGCCAAAGGCACACACTTAACAGCTGAGCCCCAGGCAC1070.12730820483533217No Hit
GTGGAGGTCCTGGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGC1070.12730820483533217No Hit
GGGTTGTGGAAGTGCTGCGGGTATGATGGGAGGATGTGACAGGAGTGTGG1050.12492861222158766No Hit
GGGGATGAGGTTTCCTGTGGGACTGGTGGAGGCAGTCACCGGCGCAGTAA1040.1237388159147154No Hit
GGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAGCCAGAT1040.1237388159147154No Hit
GCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACC1020.12135922330097086No Hit
CCACAACCCCACGCTGCAGCCCTGCCAGGGAGAGAGTTCCCCGGCCAGCA1020.12135922330097086No Hit
TCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAG1000.11897963068722633No Hit
GGGCTGGGGATGAGGTTTCCTGTGGGACTGGTGGAGGCAGTCACCGGCGC1000.11897963068722633No Hit
GCGTGGGGTTGTGGAAGTGCTGCGGGTATGATGGGAGGATGTGACAGGAG1000.11897963068722633No Hit
GGCCAGGGACTCGCGTCAGTGAGCGTCTTCGTGGAGCCCGGGCTGAGACT990.11778983438035409No Hit
CCTCAGAGGCCACAATGCAGACTTTGTTCAGCCACTAAGGGCTCTGAAGC980.11660003807348182No Hit
GTATAAGGCTTAGTCTCTAGATTCATTTTATTCTATTTTCTTTTAAATAT980.11660003807348182No Hit
GGCTGGGGATGAGGTTTCCTGTGGGACTGGTGGAGGCAGTCACCGGCGCA980.11660003807348182No Hit
ATCATACCCGCAGCACTTCCACAACCCCACGCTGCAGCCCTGCCAGGGAG970.11541024176660956No Hit
GCAGAGAGCCAGATCCCCGGACCCTGGGATCAGGACCTGAGCCAAAGGCA970.11541024176660956No Hit
ACCCCACGCTGCAGCCCTGCCAGGGAGAGAGTTCCCCGGCCAGCACCTAG960.1142204454597373No Hit
GGCGGTGTGTCATAGTTTGTCAACCATGATCTAAGAAATGTACTCTCCAA950.11303064915286502No Hit
GATTGGGGCCGGAATTACAGCAGGAGCTGAAGCTGGAGTGGGTCGAGGCC940.11184085284599277No Hit
ATCCCAGGGCGCTGGGATCCTGACCTGAGCCGAAGGCAGCCGCTTCACCA940.11184085284599277No Hit
CATTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTTAGGGGGAGAAAGG930.1106510565391205No Hit
AAGCTGGAGTGGGTCGAGGCCTAGGTGCTGGCCGGGGAACTCTCTCCCTG930.1106510565391205No Hit
GCAGGAAGGGCACCGGTGTGCTGAAGGGGCCTTCTAGCATCTTCCGCAGC930.1106510565391205No Hit
GGCGGAGGGGTGCAGGCAGGAGGAGGGGCACAGGCAGAAGAACAAGAGGG930.1106510565391205No Hit
GGACTGGTGGAGGCAGTCACCGGCGCAGTAAGAAGGCCAAGGCCCCTGAA930.1106510565391205No Hit
GTTTGTCAACCATGATCTAAGAAATGTACTCTCCAACCCAATACTTCCCT930.1106510565391205No Hit
GAGGAGGAGGGGCCAGGGGCTGGGGATGAGGTTTCCTGTGGGACTGGTGG920.10946126023224825No Hit
AGCGAAAGCAGGACATCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAG910.10827146392537598No Hit
GTGGAGGCAGTCACCGGCGCAGTAAGAAGGCCAAGGCCCCTGAAAGGCCA910.10827146392537598No Hit
GGCACAGGCAGAAGAACAAGAGGGAGGAATCGGTGAAGGAGGCAGTGGGG910.10827146392537598No Hit
CTTCTGTCTTGTCGAAGAGCAGCTGCAGGGAGCCCAGCAGCTTCTTGGCC910.10827146392537598No Hit
GAACAAGTGGAAGCTGCCCGCAAGGATCTGGACCAAGCCAAGGAGGAGGT910.10827146392537598No Hit
AGGCAAGACAGGAGCAGGAAGCATGGTTATTGGACTGGGGGCCGGAATTG890.10589187131163146No Hit
GAAGAACAAGAGGGAGGAATCGGTGAAGGAGGCAGTGGGGGAGAAGGCAA890.10589187131163146No Hit
TTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTTAGGGGGAGAAAGGCT890.10589187131163146No Hit
AGAGGGAGGAATCGGTGAAGGAGGCAGTGGGGGAGAAGGCAAGACAGGAG880.10470207500475918No Hit
ATATCACACTGTATGTTAATTACACTGGAATTAAAAACAAAGATATGCAT880.10470207500475918No Hit
AGCCAAGGAGGAGGTGTTCCGCCTACCCCAAGAGGAGGAGGAGGGGCCAG880.10470207500475918No Hit
GTCCAGAAGAGGCAATCCGGGGTGATTAGCTACTGAACCCTTTATTCCTG880.10470207500475918No Hit
ATAAAAAAGAGTGCATGTTATAAAAGAAGTCAAAGGCGGTCTTGAATAAG870.10351227869788693No Hit
CCTATAGACTCATTTATTAAGGAGCAACGAAGTCAACCAACACCAGCCAT870.10351227869788693No Hit
GAGCAGGAAGCATGGTTATTGGACTGGGGGCCGGAATTGGAACAGGGATT870.10351227869788693No Hit
GTTCTGCAGCGAGAACGCAGCGCAGGCGCTGCACCTCGGGGAGCGCATGC870.10351227869788693No Hit
GAACAGGGATTGGGGCCGGAATTACAGCAGGAGCTGAAGCTGGAGTGGGT870.10351227869788693No Hit
GTCACCGGCGCAGTAAGAAGGCCAAGGCCCCTGAAAGGCCAGGGACTCGC870.10351227869788693No Hit
ACCTATGCCTGCAAGGCCATCAGCGGGAGGAAACGAGGCAGCCGGAATCC870.10351227869788693No Hit
GTTCCTTGACAAAAAGGCTTCTCCCTGGCTTCAGTAAAAAGAGATCAGGC850.10113268608414239No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)