FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S8_R2_D12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S8_R2_D12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478966
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAA99722.0819849425637726No Hit
ATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATA47230.9860825194272662No Hit
ATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATG39540.8255283256013997No Hit
CCTTTAAAGAGGCCAAGTAAATAACCCAGAGTCATTCACTAGGAAGTGGC36760.7674866274432841No Hit
ATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATACT35730.7459819694926153No Hit
GTTATATAGGATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTC33090.6908632345510954No Hit
ATACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCT32840.685643657378603No Hit
AAACAATACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCAC25210.5263421620741348No Hit
ATATAGGATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAA25120.5244631142920374No Hit
GTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGG24470.5108922136435571No Hit
CTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATAACCCAGAGTC24050.5021233239937699No Hit
GACAAGAAGAATCAAAGTTATATAGGATTATAAACTTTGTCTTAGCCTCA21960.45848765883173337No Hit
CTGCAGGACCGTGATCTTATGGTGCTGGGATTGAGCCCCCTCTCGGGCTT21950.45827887574483367No Hit
ATTTAGGACTTAGAAGTCTGGGAGAGAGAGAGTACAAAATACTATGTTTT21120.44094987953215886No Hit
ACTTAGAAGTCTGGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTAT21040.43927961483696126No Hit
GTATTGTTTGAAGGCCTTTTCTTCTTGAGGCTAAGACAAAGTTTATAATC20030.41819252306009197No Hit
ACTCAGTATTGTTTGAAGGCCTTTTCTTCTTGAGGCTAAGACAAAGTTTA19140.39961082832601896No Hit
AAATAACCCAGAGTCATTCACTAGGAAGTGGCAGAATTATAATTAAACTC18320.38249061520024386No Hit
CTATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCACATT18200.3799852181574475No Hit
GTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGTGG17420.3637001373792712No Hit
GGATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATA16750.34971167055699154No Hit
ACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAA16530.3451184426451982No Hit
ATTATGATTTGGGCACTATTATCCTCACTTCATATTTGGGGAAACCTTTA16420.3428218286893015No Hit
GTCTGGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAA15940.33280024051811613No Hit
AAGTTATATAGGATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCT15640.32653674791112525No Hit
GTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATACTGAGTAGTGGTAA15540.32444891704212825No Hit
ATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCACATTCA15440.3223610861731313No Hit
ATCCTATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCAC15020.313592196523344No Hit
GGATAATAGTGCCCAAATCATAATTATTTTTATTTTTTTTTAAGATTTTT15000.3131746303495447No Hit
CTTTAAAGGTTTCCCCAAATATGAAGTGAGGATAATAGTGCCCAAATCAT14970.3125482810888455No Hit
ACCCAGAGTCATTCACTAGGAAGTGGCAGAATTATAATTAAACTCCATTT14940.3119219318281464No Hit
CTTCTTGTCTGTTCATTTTCTTACATTCCACATTCATATTTCCTGCTTTT14450.30169156057006136No Hit
ATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGTGGAATGTAAGAA14050.29334023709407353No Hit
GGGCACTATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAGGCCAA13610.28415378127048685No Hit
GGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGG13530.28248351657528925No Hit
GATTTGGGCACTATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAG13360.27893420409799446No Hit
GTAAATAACCCAGAGTCATTCACTAGGAAGTGGCAGAATTATAATTAAAC13110.273714626925502No Hit
ATCTTATGGTGCTGGGATTGAGCCCCCTCTCGGGCTTCCTGCTCAGGGGG12610.2632754725805172No Hit
GTTTATAATCCTATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTAC12110.2528363182355324No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA12110.2528363182355324No Hit
AGCGAAAGCAGGAGATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGA12080.2522099689748333No Hit
GGAAATATGAATGTGGAATGTAAGAAAATGAACAGACAAGAAGAATCAAA11390.23780393597875424No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC11300.23592488819665697No Hit
ATCTCATTTAATATTTAAAATAACTATGGGGGCACCTGCAGGACCGTGAT10980.22924382941586668No Hit
CTATGGGGGCACCTGCAGGACCGTGATCTTATGGTGCTGGGATTGAGCCC10950.22861748015516759No Hit
GATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATAC10570.2206837228529791No Hit
TAATTATGATTTGGGCACTATTATCCTCACTTCATATTTGGGGAAACCTT10310.21525536259358702No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC9830.20523377442240157No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC9670.20189324503200645No Hit
ATATGAATGTGGAATGTAAGAAAATGAACAGACAAGAAGAATCAAAGTTA9640.20126689577130738No Hit
GTGCTGGGATTGAGCCCCCTCTCGGGCTTCCTGCTCAGGGGGAGTCTGCT9030.18853112747042586No Hit
ATATTACAATACAACTACAGGCACGTGAGTGGCTCAGTCGGTTAAGTGTC9010.18811356129662649No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC8920.18623451351452922No Hit
GAGGATAATAGTGCCCAAATCATAATTATTTTTATTTTTTTTTAAGATTT8630.180179803994438No Hit
GAAAGCAGGAGATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAG8590.17934467164683923No Hit
TATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATAACCCAGAGTCATTCA8570.17892710547303983No Hit
GAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCA8500.17746562386474196No Hit
CTCCTACCCTTCCCCTCCTCAAATAAATAAAAATCTTAAAAAAAAATAAA8460.17663049151714316No Hit
GTCATTCACTAGGAAGTGGCAGAATTATAATTAAACTCCATTTTTACCAC8450.1764217084302435No Hit
AAGCAGGAGATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAGAG8450.1764217084302435No Hit
CATTTAATATTTAAAATAACTATGGGGGCACCTGCAGGACCGTGATCTTA8420.1757953591695444No Hit
ACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCTAG8350.17433387756124652No Hit
ATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAGAGAGTACAAAA8190.17099334817085138No Hit
GTAATATAGTGCTTTTCATCACCTATCAGTCCATTGTCTGCCTGTGGCCA8190.17099334817085138No Hit
TTATAATTCTGCCACTTCCTAGTGAATGACTCTGGGTTATTTACTTGGCC8100.1691143003887541No Hit
GGGTAGGAGGGAGAAGCAGACTCCCCCTGAGCAGGAAGCCCGAGAGGGGG7910.16514742173765987No Hit
TTGTAATATAGTGCTTTTCATCACCTATCAGTCCATTGTCTGCCTGTGGC7890.16472985556386047No Hit
ACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAA7780.16243324160796382No Hit
GAATCAAAGTTATATAGGATTATAAACTTTGTCTTAGCCTCAAGAAGAAA7760.16201567543416442No Hit
TATGTACACTATCTCATTTAATATTTAAAATAACTATGGGGGCACCTGCA7440.15533461665337414No Hit
GTAAGAAAATGAACAGACAAGAAGAATCAAAGTTATATAGGATTATAAAC7350.15345556887127687No Hit
CTATATTACAATACAACTACAGGCACGTGAGTGGCTCAGTCGGTTAAGTG7310.1526204365236781No Hit
GATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAGAGAGTACAAA7300.1524116534367784No Hit
GAATTATAATTAAACTCCATTTTTACCACTACTCAGTATTGTTTGAAGGC7290.1522028703498787No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT7220.1507413887415808No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG7210.15053260565468113No Hit
GAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCTAGTGA7120.14865355787258386No Hit
CTATCTCATTTAATATTTAAAATAACTATGGGGGCACCTGCAGGACCGTG7060.1474008593511857No Hit
AATATGAAGTGAGGATAATAGTGCCCAAATCATAATTATTTTTATTTTTT7020.1465657270035869No Hit
AAAGCAGGGGGAGCAGCAGGAAGAGGGAGAGGGACAAGCTGGCTTCCTGA7000.14614816082978752No Hit
GGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGTGGAATGT6950.14510424539528902No Hit
ACTCTGGGTTATTTACTTGGCCTCTTTAAAGGTTTCCCCAAATATGAAGT6910.14426911304769025No Hit
TTGTTGGACTGGCCAGTGGCCAAAGCCTGGGCTAGCAAAGGTACTTTTGT6890.14385154687389085No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC6810.14218128217869327No Hit
CACTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATAACCCAGAG6810.14218128217869327No Hit
ATGTAAGAAAATGAACAGACAAGAAGAATCAAAGTTATATAGGATTATAA6760.1411373667441948No Hit
GAGAGAGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAA6760.1411373667441948No Hit
GTCCTGCAGGTGCCCCCATAGTTATTTTAAATATTAAATGAGATAGTGTA6740.1407198005703954No Hit
AAATATGAATGTGGAATGTAAGAAAATGAACAGACAAGAAGAATCAAAGT6730.1405110174834957No Hit
ATTTAACAGAGAGAGGGACAGCGAGAGAGCGAACACAGGCAGGAGGAGTA6700.1398846682227966No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA6520.13612657265860206No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA6500.13570900648480266No Hit
GGGGAAGGGTAGGAGGGAGAAGCAGACTCCCCCTGAGCAGGAAGCCCGAG6470.1350826572241036No Hit
GTCCTAAACCCCCATCATATTTTCATTGTTGGACTGGCCAGTGGCCAAAG6390.13341239252890602No Hit
GGCTAATATTGTTAGTCTCTGGTTCCTCCAAAGGTTGAAGTAGGTACCGT6040.12610498448741664No Hit
GGGTTATTTACTTGGCCTCTTTAAAGGTTTCCCCAAATATGAAGTGAGGA6020.12568741831361724No Hit
GATCATGACCTGAGCCAAAGGCAGACACTAACCGACTAGGCCACCCAGAT6000.12526985213981787No Hit
CCTATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCACAT5900.1231820212708209No Hit
TTCCCATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTC5880.1227644550970215No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT5870.12255567201012181No Hit
AGTAGGTACCGTGTGGTCCTAAACCCCCATCATATTTTCATTGTTGGACT5840.12192932274942272No Hit
TAATTAAACTCCATTTTTACCACTACTCAGTATTGTTTGAAGGCCTTTTC5790.12088540731492424No Hit
GTTTAATTATAATTCTGCCACTTCCTAGTGAATGACTCTGGGTTATTTAC5740.11984149188042575No Hit
CTACTCAGTATTGTTTGAAGGCCTTTTCTTCTTGAGGCTAAGACAAAGTT5710.11921514261972666No Hit
GGAAGAGGGAGAGGGACAAGCTGGCTTCCTGATGAGCAAAAGACCCCCCT5650.11796244409832848No Hit
ATATATGGGTGCCTGGGCGACTCAGTCATTAAGCATCTGCCTTAGGCTTA5640.1177536610114288No Hit
TATTTGAGGAGGGGAAGGGTAGGAGGGAGAAGCAGACTCCCCCTGAGCAG5570.11629217940313091No Hit
GTCCATTGTCTGCCTGTGGCCATAATGATCTCCTGGCATCTAACGCATTG5570.11629217940313091No Hit
GATTAGATGTGTCGTGAAGAATTCACCTCACCCAGAGGGAGGTCTGGCCT5540.11566583014243183No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT5520.11524826396863243No Hit
CTTCTAATTGGGGGGAAAAAAAGTCTTTGTTGCTTTCATCGACAGTTTTT5510.11503948088173273No Hit
GCTCAGGGGGAGTCTGCTTCTCCCTCCTACCCTTCCCCTCCTCAAATAAA5480.11441313162103364No Hit
AGCGAAAGCAGGGGGAGCAGCAGGAAGAGGGAGAGGGACAAGCTGGCTTC5400.11274286692583607No Hit
ACTATGGGGGCACCTGCAGGACCGTGATCTTATGGTGCTGGGATTGAGCC5380.11232530075203667No Hit
CACTAGGAAGTGGCAGAATTATAATTAAACTCCATTTTTACCACTACTCA5300.11065503605683912No Hit
CTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCACATTCATATTTC5280.11023746988303972No Hit
AAGCAGGAGTTAGCCAGTCAACAGGGAAAGAAGTCTGGGGGATAAAAGAT5270.11002868679614003No Hit
GTTTTGAGATCCAGGGCATCTGGGTGGCCTAGTCGGTTAGTGTCTGCCTT5240.10940233753544092No Hit
ACACTATCTCATTTAATATTTAAAATAACTATGGGGGCACCTGCAGGACC5220.10898477136164154No Hit
CTTGTATAGTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCAT5210.10877598827474183No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG5140.10731450666644396No Hit
AGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAA5140.10731450666644396No Hit
GATTAATCCAACTGAGCAAAAAATGTAAAAAATTAAAAATTAAAAAAATT5090.10627059123194547No Hit
TTCATATTTCCTGCTTTTTTTCCTTTTGTCCAAATAGACCAAAACATAGT5080.10606180814504579No Hit
CTAGGAAGTGGCAGAATTATAATTAAACTCCATTTTTACCACTACTCAGT5070.1058530250581461No Hit
GAGAGGGACAGCGAGAGAGCGAACACAGGCAGGAGGAGTAGGAGAGTGAG5060.10564424197124639No Hit
CTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGTGGAATGTAAG4870.10167736332015218No Hit
GTCTTAGGGGGAAAAAAATGTGCCTTTGGAATGTACCATTGCTCACACAC4870.10167736332015218No Hit
TTCTTACATTCCACATTCATATTTCCTGCTTTTTTTCCTTTTGTCCAAAT4860.10146858023325246No Hit
ATTTAAAATAACTATGGGGGCACCTGCAGGACCGTGATCTTATGGTGCTG4840.10105101405945306No Hit
AAATAATTATGATTTGGGCACTATTATCCTCACTTCATATTTGGGGAAAC4840.10105101405945306No Hit
GTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCTAGTGAATGACTCT4820.10063344788565369No Hit
CAATACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTTC4800.10021588171185429No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)