FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S8_R2_C11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S8_R2_C11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788049
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT76840.4297421379391728No Hit
GTCCATATGTATTTGGCTAAATTGGACAGTGTTTTCCCAAAAGCAAATGT40030.22387529648236712No Hit
CATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCA25860.14462690899410474No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA24550.1373004878501652No Hit
GTATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGA24010.13428043638625117No Hit
ATATAGCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCG21920.12259171868332468No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT20090.11235709983339383No Hit
GTGTAACTGATTCCCAAAAACGAAGAGAAATTCTTTCAAGGAGGCCTTCC19750.11045558594870722No Hit
GTATAAAGTCCCGCCCAAAACTGTACCACAGAAAGAAAGCTGGGTAACTT19660.10995224403805488No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA19620.1097285365222094No Hit
GTCTAATATCTGAAGAATGACAGATTCACGCACATATACACAGAATGTGG18810.10519845932633837No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)