FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S8_R2_C09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S8_R2_C09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716685
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC80040.46624744784279004No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG48280.28123971491566596No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA44940.2617836120196775No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA37790.2201335713890434No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA37690.2195510533382653No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT34890.20324054791647858No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC34500.200968727518444No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT34460.20073572029813275No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG32010.1864640280540693No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT31480.1833766823849454No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA30130.17551268869944106No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT29440.1714933141490722No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT27630.1609497374299886No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG27560.16054197479444396No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA27320.1591439314725765No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT27190.15838665800656498No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC26760.15588183038821915No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT26700.1555323195577523No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA26190.152561477498784No Hit
GTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAAAA25070.14603727533006927No Hit
GTCTATATGGCACCAACTTTCTTCCACCATTTTCTTTAGTTTCAGTGCCT24860.1448139874234353No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT24740.14411496576250157No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT24310.14161013814415574No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT23850.1389305551105765No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG23400.13630922388207506No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA23070.13438691431450733No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA22960.1337461444586514No Hit
GTCTAGAGCTGAGAACAGGATTGGGTATTCTAAATCCTTTTATCTCTGTT22950.13368789265357361No Hit
GTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATA22630.13182383489108368No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA22610.13170733128092807No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG21680.12628991340869175No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC21640.12605690618838053No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA21470.12506662550205774No Hit
GTTAAAAGTGTGATGTTGCTATCTAGTATATAAGCATGCAAATCCATGAA20970.12215403524816724No Hit
ATATCACACTGTATGTTAATTACACTGGAATTAAAAACAAAGATATGCAT20800.1211637545618445No Hit
CAGTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAA19710.11481430780836321No Hit
ATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGTAAA19460.11335801268141797No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG19330.11260073921540645No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA19160.11161045852908368No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC19050.11096968867322775No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA18830.10968814896151595No Hit
ATATAGTATCTGGCCCAGGCATACTATAATTTGACTGTTAGCAGATTCTT18680.1088143718853488No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC18460.10753283217363697No Hit
ATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGC18420.10729982495332575No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA18260.10636779607208079No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG18230.10619304065684736No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT18030.10502800455529115No Hit
CTCATGAGCAAACCGCACATCAGAGGAATTAAAAGATGACCTGATGGAGA17840.10392122025881277No Hit
CATAAAAAGTTATTCTATTATTCATGCTTGTATTCTGGACTGAGATTAGT17720.10322219859787904No Hit
GAAATATAGTATCTGGCCCAGGCATACTATAATTTGACTGTTAGCAGATT17470.1017659034709338No Hit
ATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTC17450.10164939986077817No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC17330.10095037819984447No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)