FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S8_R2_B10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S8_R2_B10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542127
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG42140.2732589468960728No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAAGATCAAAT39620.25691788030428103No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT38840.2518599311211074No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA29090.18863556633143705No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA23830.15452683209618923No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT20950.1358513274198558No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC20530.13312781632122386No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG20230.13118245125077246No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG19810.12845894015214052No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA19000.12320645446192174No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA18420.11944541532571572No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)