FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S8_R2_B03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S8_R2_B03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615357
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATAGTTGGTTTTGGGGGGAAATTTGCAACCTCTTTATCATACCAG48330.2991908290241724No Hit
GTATAATGAGATGGTTGATAATAGGACTTCATGACTCTATGTCTGCAAAG41310.255732943244125No Hit
TCATATCTGAGAAGCCATCTCTTTTGAAAGTTTCTAGTTGACATGCAGTA40660.25170906493115763No Hit
CATCATATCTGAGAAGCCATCTCTTTTGAAAGTTTCTAGTTGACATGCAG40620.25146144165035966No Hit
GTATTTGATTATGAAGACATTCAGATTGGATGAATTGGGAGACAGAATTT39150.24236128608103347No Hit
CACTTAGTGTTTGATGCGTTTTCATGAAGAAATGCTTCTCAAATTTGTTG34160.2114702818014841No Hit
GGTATGATCATTTCAAATTACTGATATGATACAGCAAACTGTCTTTGGAT29540.18286979286931618No Hit
CTCTTTATCATACCAGAAGTCTTCTCAACTCATCCCAAGGACTTAATGGA25850.16002654521570153No Hit
GATATTACCTACCGAATTTCTAAGTAGATAACCCAGTAATCGTAGAGATA24800.1535264340947543No Hit
ATCTAGTATCTTGTTGTTGTTTTTTTTTCTTCTCATAAACTTGAAAATAA23480.14535486582842058No Hit
GGATAAAGTGAGAAAGTGTAAATTCTTCCAACAAGAAGCAAATTTCACAG22400.13866903724687485No Hit
ATCAAATACTGCATGTCAACTAGAAACTTTCAAAAGAGATGGCTTCTCAG20950.12969269331794767No Hit
TCTTGATACAGAAACCTGAACCTACCTATTTTTTTTAAGTGCTTCAAAGA19680.12183065415261148No Hit
CTCTTGATCTTTACCTAAGATCAACTTTCCAGCTACCATTTTTCTATTGA19650.12164493669201298No Hit
ATATGATACAGCAAACTGTCTTTGGATCCAGCCAGACCTAGGTTCAAATA18970.11743534091844714No Hit
GTACTAGACATATTTTGCAGATAAGGAAACTGGCTGAAATATCAAGTAGC18950.11731152927804812No Hit
AACCTGAACCTACCTATTTTTTTTAAGTGCTTCAAAGAAATGTCTCCCTT18790.11632103615485617No Hit
TAATAGGACTTCATGACTCTATGTCTGCAAAGAACATGCACCATGAATAC18150.11235906366208832No Hit
TCATAACGCAAACCTAATCAAATGGAAATTCTGTCTCCCAATTCATCCAA18060.11180191128029285No Hit
CCTCCAGGGTCTGGGTGTCCAGCAGCAAGCCACACCAGGGGAACAGGCAG17970.11124475889849736No Hit
GGGTAAGGGTGTGAGGCCTGGGCTCTGGGCCAATTCTATCACCACTTAGT17580.10883043191071695No Hit
TTCCTGGGTCGTACCTCCAGTGTCAGGGGATCCCCCAGGGCTCCGTCCTG17520.10845899698951997No Hit
GTCTTAAATGCTTCTCCTAAAAAAGACAGAAAAAAGTTTCAAAAGATTGG17350.1074065980461285No Hit
ATATTATGTTAATTGCTCAGAAATTCAGCAAGATGGTGTTTGGGACCCGT17230.10666372820373454No Hit
GATAAATACATATTGATATGGAAAGTCTCAAAACATGATCAAATACCCTG16190.10022552290298677No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)