FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S8_R2_A04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S8_R2_A04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338793
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCA19460.14535480839831103No Hit
GTGTTACATTGTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAG17740.13250741526135854No Hit
TCTTATATGTTCTTGATTTTAAGACAAAGGCTGCATGATTTGAGCTAATG17040.12727882503120347No Hit
ATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCAAAAAATCTAG16450.12287187040864421No Hit
GTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAGGGCTGTGAAA15710.11734450359390884No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)