FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S7_R2_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S7_R2_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCACTACAGCGCCAGGAGCTGCCCACTAATCTCCCTTCAACAATTACA74.3478260869565215No Hit
GTATCAAACCCACTACAGCGCCAGGAGCTGCCCACTAATCTCCCTTCAAC53.1055900621118013No Hit
AAGCAGGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTCGG42.484472049689441No Hit
GCAGGGGTTGGGTATCAAACCCACTACAGCGCCAGGAGCTGCCCACTAAT31.8633540372670807No Hit
GCCCACTAATCTCCCTTCAACAATTACACAAGGGGCCGACTCACTGTGAC31.8633540372670807No Hit
AGTCGGCCCCTTGTGTAATTGTTGAAGGGAGATTAGTGGGCAGCTCCTGG31.8633540372670807No Hit
GAGCTGCCCACTAATCTCCCTTCAACAATTACACAAGGGGCCGACTCACT21.2422360248447204No Hit
CCACTAATCTCCCTTCAACAATTACACAAGGGGCCGACTCACTGTGACCC21.2422360248447204No Hit
GTACTATTTCACTCCCCTCCCGGGGTACTTTTCACCATTCCCTCACGGTA21.2422360248447204No Hit
CCCTTCAACAATTACACAAGGGGCCGACTCACTGTGACCCTGACCAGGGA21.2422360248447204No Hit
AAAGCAGGCTACCACGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACC21.2422360248447204No Hit
TCCCTGGTCAGGGTCACAGTGAGTCGGCCCCTTGTGTAATTGTTGAAGGG21.2422360248447204No Hit
GTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTCGGCCCCTTGT21.2422360248447204No Hit
GCCAGGAGCTGCCCACTAATCTCCCTTCAACAATTACACAAGGGGCCGAC21.2422360248447204No Hit
GGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTCGGCCCCT21.2422360248447204No Hit
GCATATCTTTGACTATTTTCTATCGTTCTAACAGATCCGTTAGTATAATC10.6211180124223602No Hit
TGTCCAAAGAGGCCACGGACACAGAGATAACCCCTGTGCGCTGCTGGGAA10.6211180124223602No Hit
ACCTCATCTTTTAAAACATGCATTTCTCTCTTCTTCTTTTTTACTTCTTA10.6211180124223602No Hit
GCGAAAGCAGGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGAG10.6211180124223602No Hit
GCTCAAAAGACGGCGTTCACTGCCATTATCGAGGATAACCGACCGGGCTG10.6211180124223602No Hit
CTTCCCTGGTCAGGGTCACAGTGAGTCGGCCCCTTGTGTAATTGTTGAAG10.6211180124223602No Hit
GTGCATTGATATCCCGTCGAAGCGCCTTGAATACCGCCTGTTTCAGTCGA10.6211180124223602No Hit
ATATTATAATAGAAGACATTAGTAAAAAGATACTGAAATGTTTGGAGATT10.6211180124223602No Hit
GCGCCAGGAGCTGCCCACTAATCTCCCTTCAACAATTACACCTGTCTCTT10.6211180124223602No Hit
GGATTCCTCGGGCCCCGTGCTACTCGGGCAGCCACTCAACAGCGGCGGAA10.6211180124223602No Hit
GGCCCCGTGCTACTCGGGCAGCCACTCAACAGCGGCGGAACGCATTACAA10.6211180124223602No Hit
ACCCACTACAGCGCCAGGAGCTGCCCACTAATCTCCCTGTCTCTTATACA10.6211180124223602No Hit
GCAAAAGCAGGCTAACACGTATTTCATAGCCTCTGACTGCCAAGGAATCC10.6211180124223602No Hit
GTCTGACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATA10.6211180124223602No Hit
AAAGCAGGGGCTCAAATTAGGAAGCCTCTGTAGTAATCTTGCATAAAGAC10.6211180124223602No Hit
GTTGATTCCATTCCATTCCATTCCATTCCATTCCATTCCAGTCCAGTCCT10.6211180124223602No Hit
GTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCTGTCTCTTATACACA10.6211180124223602No Hit
GGTCAGGGTCACAGTGAGTCGGCCCCTTGTGTAATTGTTGAAGGGAGATT10.6211180124223602No Hit
GTTGAAGGGAGATTAGTGGGCAGCTCCTGGCGCTGTAGCTGTCTCTTATA10.6211180124223602No Hit
CTACCTGTACAGCAACAGGATAGCGACAATACCGCACTGCTACTTCAAGT10.6211180124223602No Hit
CAATTACACAAGGGGCCGACTCACTGTGACCCCTGTCTCTTATACACATC10.6211180124223602No Hit
GAAAGCAGGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTC10.6211180124223602No Hit
ATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTCGGCCCCTTGTGTAAT10.6211180124223602No Hit
GTCGGCCCCTTGTGTAATTGTTGAAGGGAGATTAGTGGGCAGCTCCTGGC10.6211180124223602No Hit
CGTATAGACGAACAATCTTGGATGGTTGACCGTAGAGGGTGAGAGTCCCG10.6211180124223602No Hit
TAAGAAAAGTATCAGAACAAAAAAGCACGAAGATTACTAATGTCAAACGA10.6211180124223602No Hit
AGTTAATACAAACTGAAGTTTAAGATTCTATTATAATTACGTAGGTAAAA10.6211180124223602No Hit
GGGTCACAGTGAGTCGGCCCCTTGTGTAATTGTTGAAGGGAGATTAGTGC10.6211180124223602No Hit
GTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGACTGTCTCTTATA10.6211180124223602No Hit
AGCAGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGA10.6211180124223602No Hit
TTCAGGTACTATTTCACTCCCCTCCCGGGGTACTTTTCACCATTCCCTCA10.6211180124223602No Hit
GTAGTGGGTTTGATACACAACCACTGATTTCGCCTGTCTCTTATACACAT10.6211180124223602No Hit
GTTGGGTATCAAACCCACTACAGCGCCAGGAGCCTGTCTCTTATACACAT10.6211180124223602No Hit
GTGTAATTGTTGAAGGGAGATTAGTGGGCAGCTCCTGGCGCTGTAGTGGG10.6211180124223602No Hit
ATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGT10.6211180124223602No Hit
GTCACAGTGAGTCGGCCCCTTGTGTAATTGTTGAAGGGAGATTAGCTGTC10.6211180124223602No Hit
AAAATACCACGTCGATCGAGAATACACGGCCATTGGATAGGGACGCTCCC10.6211180124223602No Hit
TCCCTGGTCAGGGTCACAGTGAGTCGGCCCCTTGCTGTCTCTTATACACA10.6211180124223602No Hit
GGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGAT10.6211180124223602No Hit
CTCTGGGCGTGTCTGCCGGCACGTCGCAGTGAGGTGCGGGCGTATAGACG10.6211180124223602No Hit
ATCTTGGATGGTTGACCGTAGAGGGTGAGAGTCCCGTAGTTGTAATGCGT10.6211180124223602No Hit
CCATCTTCCCTGGTCAGGGTCACAGTGAGTCGGCCCCTTGTGTAATTGTT10.6211180124223602No Hit
GCCTCTGACTGCCAAGGCATCCACCGTATGCGCTTCTTCACTTGACCCCC10.6211180124223602No Hit
ACTTTTCACCATTCCCTCACGGTACTGATTCACTATCGGTCAACAGGTAG10.6211180124223602No Hit
ATCGAAACCTAATGATCTCTCCTATTTGTGAAGTTCCCGCTCCAAACACC10.6211180124223602No Hit
TCTAGGACCCGCAGAAAGTATTGAATGGGTAGAGTCTAAGTACGGGAGAT10.6211180124223602No Hit
CTGTGACCCTGACCAGGGAAGATGGAGATGACACTCCTGCTGTCTCTTAT10.6211180124223602No Hit
GCGAAAGCAGGGGTTGGGTATCAAACCCACTACAGCGCCAGGAGCTGCCC10.6211180124223602No Hit
TTGGGTATCAAACCCACTACAGCGCCAGGAGCTGCCCACTAATCTCCCTT10.6211180124223602No Hit
GCGCCAGGAGCTGCCCACTAATCTCCCTTCAACAATTACACAAGGGGCCG10.6211180124223602No Hit
CTACAGCGCCAGGAGCTGCCCACTAATCTCCCTTCAACAATTACACAAGG10.6211180124223602No Hit
ACAGGGTGTGTCTTTCACACCGTATGTTGGATATCAGAGAGGCTAGATAT10.6211180124223602No Hit
AGCGAAAGCAGGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGA10.6211180124223602No Hit
GTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCGTATGCGCTTCTTCA10.6211180124223602No Hit
GTACTGATTCACTATCGGTCAACAGGTAGTATTCAGGCTTACCAGGTGGT10.6211180124223602No Hit
CCTCCAAATAGCTAAAAGGTTAATCAACCCAAATGTGCTCTGATGATGAA10.6211180124223602No Hit
AATACATAAAGCCCAAATATCTAATTGTACATCTACCACTTTTTCAAAAA10.6211180124223602No Hit
AGCGAAAGCAGGGGTTGGGTATCAAACCCACTACAGCGCCAGGAGCTGCC10.6211180124223602No Hit
AATCTCCCTTCAACAATTACACAAGGGGCCGACTCACTGTGACCCTGACC10.6211180124223602No Hit
AGCAGGGGTTGGGTATCAAACCCACTACAGCGCCAGGAGCTGCCCACTAA10.6211180124223602No Hit
AAAGCAGATGGGGCTAAACCGTATGTGTGTGATACCCGGCAGGGGTTGCA10.6211180124223602No Hit
CCACCGTATGCGCTTCTTCACTTGACCCCTGCTTCTGTCTCTTATACACA10.6211180124223602No Hit
ACTATGGACGGGATCTTCCCATTCCTGAAGTCTGCTTAACAGGGGAGCGA10.6211180124223602No Hit
CTTGGATGGTTGACCGTAGAGGGTGAGAGTCCCGTAGTTGTAATGCGTTC10.6211180124223602No Hit
CTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGAGTTGGAAC10.6211180124223602No Hit
CATTTACCCTTGAAAGCTTAAGGTGCTTCTTAATGTCACAGCAAATAATT10.6211180124223602No Hit
CGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACCTGTCTCTTATACA10.6211180124223602No Hit
GCCATCCGGTGCGGACACCAGGATCACCTCAGCGTCGATGCTGCGCGCCA10.6211180124223602No Hit
AAACTATGCCACCCAGCATTCCATAGGCTGAGGTATTTCAGACACAACCA10.6211180124223602No Hit
ATTACACAAGGGGCCGACTCACTGTGACCCTGACCAGGGAAGATGGAGAT10.6211180124223602No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG10.6211180124223602No Hit
CATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTCGGCCCCTTGTGTAA10.6211180124223602No Hit
CTACCACGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCGTATGCGA10.6211180124223602No Hit
CAACTGAGCCTCCCAGGTGTCACTAAGCTGTGCTATTTCAAAGTCCACAA10.6211180124223602No Hit
CGGCTGGGACAACCTGCTGAAGAACCTCGGCGGCGACAACCTGCAACTCA10.6211180124223602No Hit
ACTGTGACCCTGACCAGGGAAGATGGAGATGACACTCCTGCTTCTGTCTC10.6211180124223602No Hit
AAGCAGGCTACCACGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCT10.6211180124223602No Hit
CCCTCACGGTACTGATTCACTATCGGTCAACAGGTAGTATTCAGGCTTAC10.6211180124223602No Hit
GCCCCGTGCTACTCGGGCAGCCACTCAACAGCGGCGGAACGCATTACAAC10.6211180124223602No Hit
CACACACATCAGTGTAGGACTATGTTACAATAAATGTGGATCTTTAGACA10.6211180124223602No Hit
GGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCG10.6211180124223602No Hit
AAATTACGCACTCTTAGCGTGCGTAAGGCCCACGCGCTGTACGTAAAGTT10.6211180124223602No Hit
AATCAAAATAGTTAAATACTGAAACACAATGAAATCAAAATGAACATCGT10.6211180124223602No Hit
GGAGATTAGTGGGCAGCTCCTGGCGCTGTAGTGGGTCTGTCTCTTATACA10.6211180124223602No Hit
CATTACAACTACGGGACTCTCACCCTCTACGGTCAACCATCCAAGATTGT10.6211180124223602No Hit
GTACATGAATAGAATCAGTAAATTTGTTGAAATATAGTATCAGGCCCAGG10.6211180124223602No Hit
CAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTTC10.6211180124223602No Hit
TTTCATGAATAATTATTGAAAATTTCCTATTACCCATCTCAATATCACAC10.6211180124223602No Hit
GAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG10.6211180124223602No Hit
GCAGGAGTGTCATCTCAATCTTCCCTGGTCAGGGTCACAGTGAGTCGGCC10.6211180124223602No Hit
GAGGTATACAGAAAGTCAGTCTGGAAAGAGGAATTCAACATATCTGCTCT10.6211180124223602No Hit
GAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTCGGCCCCCT10.6211180124223602No Hit
ACCTTAAGGAACGAGACATCAGAAATGAACGTGTCACCAGGCGGTCTGTG10.6211180124223602No Hit
CTGTGACCCTGACCAGGGAAGATGGAGATGACACTCCTGCCTGTCTCTTA10.6211180124223602No Hit
GGGGTCCTCATCGGAGGACTTGAATGGAATGGTAACACGGTTCGAGTCTC10.6211180124223602No Hit
CGGTGGAAGCCTTGGCAGACAGAGGCGATGAAAGACGTGGTAGCTGTCTC10.6211180124223602No Hit
ATGCTCCCGCGGGAGGGTAGCGGTGAAGTGATCACCACGGCCCTGCCATT10.6211180124223602No Hit
AAATAACATTCGAAGCAACTGGAAATCTATTTGTACAGAGATATGCATTC10.6211180124223602No Hit
GTTTCAGGTACTATTTCACTCCCCTCCCGGGGTACTTTTCACCCTGTCTC10.6211180124223602No Hit
AAGCAGGAGTGTCATCTCAAACTTCCCTAGTCAGGGTCACAGTGAGTCGT10.6211180124223602No Hit
TTTCTAATTTTAACAAAACTTTTAGGCGAAAATGAATAATAAATTTATCC10.6211180124223602No Hit
AAGCAGGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTCTG10.6211180124223602No Hit
CTTTAGTGATCTGTGGAATAACATAAATATCCATATAAGTTTTTATAAAT10.6211180124223602No Hit
GGATGAAGACGCCGACAACGTCCTCGGGCGCCGCCGCCCGCAACGCCCGC10.6211180124223602No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT10.6211180124223602No Hit
AAGCAGGGCGGGACGTGCTGTTCGACGTCGATTGGCAGGGCGCGCGGGCG10.6211180124223602No Hit
CTGTGACCCTGACCAGGGAAGATGGAGATGACACTCCCTGTCTCTTATAC10.6211180124223602No Hit
GTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTCGGCCCCATGT10.6211180124223602No Hit
AAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAA10.6211180124223602No Hit
ACTCACTGTGACCCTGACCAGGGAAGATGGAGATGACACTCCTGCTTTCC10.6211180124223602No Hit
ACTTATAGGACCCCTCAACGGTTCACTTAACCTACACTTCTATATACAAT10.6211180124223602No Hit
GTTGTGGGCCCACTCTGGGCGTGTCTGCCGGCACGTCGCAGTGAGGTGCG10.6211180124223602No Hit
CCCACTACAGCGCCAGGAGCTGCCCACTAATCTCCCTTCAACAATTACAC10.6211180124223602No Hit
CACTAATCTCCCTTCAACAATTACACAAGGGGCCGACTCACTGTGACCCT10.6211180124223602No Hit
GAGCAAAAGCACGTCAAATATATTCAATAAGGAGAGAATAAAAGAACTGA10.6211180124223602No Hit
GGGTTGGGTATCAAACCCACTACAGCGCCAGGAGCTGCCCACTAATCTCC10.6211180124223602No Hit
GGGTCACAGTGAGTCGGCCCCTTGTGTAATTGTTGAAGGGAGATTAGCTG10.6211180124223602No Hit
CTCGGGCCCCGTGCTACTCGGGCAGCCACTCAACAGCGGCGGAACGCATT10.6211180124223602No Hit

[FAIL]Adapter Content

Adapter graph