FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S7_R2_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S7_R2_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCCTACTAGTCCAAGCGTTTCGTCATCATCATCATCATCATCTAATT22.4691358024691357No Hit
CGGCGTTGAGCTTGCCGAGGGCGACGATGCCCTGCCCTTCGCGGAACAGG22.4691358024691357No Hit
GGACCAGCGTGGGCAAGACCGTAGTTGCAGCAGTTGAGGCCATGGAGTGG11.2345679012345678No Hit
CCATAGATTTATTGTCAAGATCAAGTAAGAGAACATGCCCAAGACTTACT11.2345679012345678No Hit
GATCTAAAGCAGTGTGACAAAAACATAATGTAATCAAACACAATGTCAAG11.2345679012345678No Hit
ATTTTCACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACA11.2345679012345678No Hit
CAGATATATCCTGACAGATGAGCATTAAGCAGTAGGTTATTGTAAAGAAG11.2345679012345678No Hit
AGGCACTGGAACAACAGCGGCCCCGCGAAGCGGCGGCGCTGTATCTGTCT11.2345679012345678No Hit
CAATTACGGGGGTGACTGGGGCCTGCCGCCCTATCCGGGTGTTGCCTGTG11.2345679012345678No Hit
GGGTTGAAGGAACGGACCCACCTTGCTCGCCCCCGACCATATCTTTTTAA11.2345679012345678No Hit
GTGTGAGTTCTTCGTATATTATTTGGGAATTAACTTCTTACCAGATATGT11.2345679012345678No Hit
CGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAA11.2345679012345678No Hit
CCGTTGGTGGCCCCTTCTTGAGGAACTTATTGAGCTTGTCGAACTTCTTG11.2345679012345678No Hit
TTAGGACTCTGAAGGATCACAAGCTATAAGGACCCTTACTGCAACCCAAG11.2345679012345678No Hit
GAAACATATCGTGGCAATAGGGGCGCCAACCACAGTCATACGCTCAGATC11.2345679012345678No Hit
CTGGTAAGGATTGCTACAAAGGTTAGCGTACGAGCATAGAAACTGACAGT11.2345679012345678No Hit
CGCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11.2345679012345678No Hit
GACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAGTGAGC11.2345679012345678No Hit
GAAGAGATCTCTGGGAAATGAATCTGCAGCTCCTCCTGTATCCATAATAG11.2345679012345678No Hit
CAAGTTTGGCAATACCGGACCATCATTGTAGCACTCTGCCTGCCCTCTAG11.2345679012345678No Hit
GCTTTGTTGCGGCCAATCGTGGCCATTGAACCTTTGTCGTTATAAACAAA11.2345679012345678No Hit
TTCTGTTCCTTCTCCTGTTCCCCTTTCAGATGGCAGTCGGATTAAAAGTA11.2345679012345678No Hit
GCATGGCAAACAGGCTGGGCTGGAATTGGTAGCCGTCAGATTCGGCTTTC11.2345679012345678No Hit
ATTTGTAAATATCAACTGTATCATTATTTTTCATCCGACAACATCAAAGA11.2345679012345678No Hit
ATTTCAGCATAGTAGATCGTATAGTATCAAAGTATTATTCTAATGTTAAA11.2345679012345678No Hit
AAGCAACACCTTCTGTTCCTTCTCCTGTTCCCCTTTCAGATGGCAGTCGG11.2345679012345678No Hit
GCTCAATGCCTCCTGTGCAGCCATGGATGCCGGTCAGCGGATCCCAATGA11.2345679012345678No Hit
GCCTTCTCTTCGGCAAAGGACGCTGCCGCCAGTGCGAACGCAGTGCCCAT11.2345679012345678No Hit
GCAATAATCTGATTTCTACAACACATTTATCATTATCTAACAATCTGGCC11.2345679012345678No Hit
ATCTGATATGTTCATCTAGGTCAAGCTGAAGTAATAAGTGTTGCCGTAAT11.2345679012345678No Hit
TACTCAATGGGAAAAGGATACTCTCTTCAATAACTGATGTGTTGGGAAAA11.2345679012345678No Hit
GGGCAAGAAACAATGGACTGGAGAGTGGCAACTGGAACACTGGCCAGAAG11.2345679012345678No Hit
ATTTTCTTCTGTCTTTCCTTCCTTCTGGATTCCAGTTATACCAAATGATA11.2345679012345678No Hit
GGTCCGGACAGAGGCATGAACTCAGAAGTATTTTTCACCACGACGGACGG11.2345679012345678No Hit
GTATTTTCGGTACTCATAAGTCCCATTTATGACCCTAAACATTCACTGCT11.2345679012345678No Hit
CAGTAAAAGTTTGATATTTCAACTTTGTAATAAACTTAGATCTACTCCTC11.2345679012345678No Hit
AGGATATGCAGCAGACCTCAAGAGCAAACAGAATTCCATTCACTAGATTA11.2345679012345678No Hit
TCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT11.2345679012345678No Hit
GATAGAAACGATGGAGTAAATAAAATTCACAAACGCAAGCAAATCCAAAC11.2345679012345678No Hit
ATGAAAGACAATCTCCTTAACTTTAAATAGGTCTAGGTTTATTGTGTTTA11.2345679012345678No Hit
GATTAAAAGTACTCCTCTTCCACTCTCTCCTACTAGTCCAAGCGTTTCGT11.2345679012345678No Hit
CACTAAATTTATTTTAATTTTAACACTACAGATCATAATTTTAAAAAACC11.2345679012345678No Hit
AGACTAGACTAAGATACAAAGGAGGGCCTTGAGCAAAATGAGGATGAAGA11.2345679012345678No Hit
AGAATGTGGATACCATGGCCGCCAATACGCTGGAAGTGAGCTTCAGATAC11.2345679012345678No Hit
ATTGGCTATTGGTTATTGTGATTGGTGATCAGTTACTGAGATCTCTTAAG11.2345679012345678No Hit
CACCTTGGCCGTCGTAAATTTTAACCCAGCCATAAATTTCTTTCCTTTAT11.2345679012345678No Hit
GTCTTGGGCTAGGCCGGGTTTGGAGCGTCGGCTCCAGAGCGCCGTGTGAT11.2345679012345678No Hit
TTCTCTCACTGCCCTTGCCTCCTCAGCCATTTGGCCATTTTGTGGAATAG11.2345679012345678No Hit
CCCCCAACCACACAACTTTATGAGGAGCCAAGACTGAGGCCTGTCTCTTA11.2345679012345678No Hit
TTGATAGGCCGGGTGCTGATGATCCAGCGCCGTGCCAATGGCGCGGCTGC11.2345679012345678No Hit
GTCTCAATCAGAATGCAGATGCATATGTTTTTGTGGGGTCATCAATATAC11.2345679012345678No Hit
ATGTATTTCTTCTAAGGGAAGGTAGAGTGCTGCATTGTAGTTCTGGATTG11.2345679012345678No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC11.2345679012345678No Hit
CAATGAGAGCCTCAACATCTGTGTTCTTTCCTGCAAAGACACTTTCCATT11.2345679012345678No Hit
ACCCAATACACGCCCAGTATTGACGTAGTCAGACAGCTCAAACTTCGACG11.2345679012345678No Hit
CTCTAACACCAACCTCCTAAGCCTCAAAAACAAGCACTTATAAAAATCCT11.2345679012345678No Hit
ATAAAGAAAATAGCTTGCATAAAATCTGAACATAATGCCACAACTAAACA11.2345679012345678No Hit
AACTTGTAGATATGTGGAACCAACTTTTAAGTATCAGATTTCCATTAACA11.2345679012345678No Hit
CCTTGATACTACTAAGGGATTTCACTGAGGAGGGAGCAATAGTTGGATAA11.2345679012345678No Hit
ATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATT11.2345679012345678No Hit
GAACTGTTCTTCAACCGATAATGACCAGGGATGCAGCACCGGCTTGATAT11.2345679012345678No Hit
AGAGAAGGCAAAAAAAAAAATCACACATAAATTAAGTAAATCGCAATGTC11.2345679012345678No Hit
GGTTTAGAGATTGAGATTTAAATCTCAAAGATAAAAGTAAAACCGGTTTT11.2345679012345678No Hit
TTGGAAAATTTTGGCTATTAGTCAAATATTTTCTTCTGTCTTTCCTTCCT11.2345679012345678No Hit
GGGTACCCGAGCCAGGGCGAATCACGCCAGAATCCACAAATCCCACAAAA11.2345679012345678No Hit
GAAATGTGCCAACGCACACAGAGTGGAGGAGTAAGTATGCTGGACATACT11.2345679012345678No Hit
TTTTTTTAATTTCCTCCTAGATGTAATATTCCACTGTCCATTCAGTTCTG11.2345679012345678No Hit
GATCCAGGCCGCGATTGACGTACTCAAGCTGGGGAGCGATGCGGTCAAGG11.2345679012345678No Hit
GCCACCAGCACCGGGATCGCGAACACCAGGAACCACGCCGCGGCCACGAG11.2345679012345678No Hit
GAGTTTGAGTTCAGTGCACATTTGGATGTAGAATCTCCCGATTCCACCAA11.2345679012345678No Hit
TAAATGAAAATGAGCTCTCTTAGGTAGAATAAACTTTAAAAGGATATGCA11.2345679012345678No Hit
GATTAATGTTGGGAAGCGTGGGTGACTCAGACGCAAAGTCTCTGCCTTTG11.2345679012345678No Hit
ATTCTCAGTAATCCTTGTATTCCCATTGGTGGCTGTTTTTTTTCCCTGCA11.2345679012345678No Hit
ACCAAAAGAATGGTGACAAAAACATATTGGACTCCAACACCATCTCAATC11.2345679012345678No Hit
GAGATTTGAATTCTCACTCCTTAGAATTTTTCATTGGGAGTAGATCTAGC11.2345679012345678No Hit
GACGGATGGTATGTGTTGGGTGAAGGGTGTGAGTTCGGGGGGCAGGCATG11.2345679012345678No Hit
CCTATAGGAGCGCAATATATTCAACAGAATCACATTTGACCGAGCAACGA11.2345679012345678No Hit
CTCTCAGCCACACCTACAGAAAAAGAACCTCATAACAAACAGTATATAAT11.2345679012345678No Hit
GTCCACAACAAGCACATTACCGAGACTCAAAAATATCATTACACACCCAA11.2345679012345678No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)