FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S7_R2_E09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S7_R2_E09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615542
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA31500.19498100327939477No Hit
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA25940.1605653087323016No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT24010.14861885361073868No Hit
ATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGACA21440.13271087969238807No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA20850.1290588545515994No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT20070.1242307535180144No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC19780.12243569031321996No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT19680.12181670300122188No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT19370.11989784233402782No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC18140.11228429839645146No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT17600.10894176691166185No Hit
GTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGA17120.10597062781407107No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT17100.10584683035167144No Hit
GGTCTGAAAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAAT16560.10250429886688184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)