FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S7_R2_A09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S7_R2_A09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525873
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA40480.2652907548662307No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT36050.23625819448931856No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG33940.22243004496442365No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA26870.17609591361797478No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG15290.10020493186523388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)