FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S7_R2_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S7_R2_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296189
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGACAGAGTCTGGCACCCAGAGACCCCTCTCCGTGTGGGGTATCTC5370.18130315440478884No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3910.1320103042314198No Hit
GTGTAGGCCAGGGCGTTGGTTAACACTGGACAGGAGTCCCAAATGCCAAA3150.106351012360351No Hit
CTGTCTACACAGAGCGGAGCCTAAGCAAAGCTCTGAAATCTCTGCAAACA3150.106351012360351No Hit
GTGCTAGAGAGAGCACAAAGACTGCAGCATTTGCAGCTGCTGAGTGAAAT3000.10128667843842952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)