FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256
Sequences flagged as poor quality0
Sequence length151
%GC65

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCTCA665.254777070063694No Hit
ATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCT443.5031847133757963No Hit
GCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGGCCCACCGGGACGCGA292.3089171974522293No Hit
GCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCC272.1496815286624202No Hit
GATTATCCGCGGCCTCAGTCGGCCCACCGGGACGCGATCGCGCGCACCTC262.0700636942675157No Hit
GGATTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGGCCCACCGGGA262.0700636942675157No Hit
GGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCG251.9904458598726114No Hit
AAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTAT251.9904458598726114No Hit
GGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGGCCCAC251.9904458598726114No Hit
ATTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTG241.910828025477707No Hit
GATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCC221.7515923566878981No Hit
AGCGAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGG211.6719745222929936No Hit
GAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATT211.6719745222929936No Hit
GAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGG211.6719745222929936No Hit
GGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGG201.5923566878980893No Hit
GCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTC161.2738853503184715No Hit
GATTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGGCCCACCGGGAC161.2738853503184715No Hit
CAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCG161.2738853503184715No Hit
GCCCACCGGGACGCGATCGCGCGCACCTCCGCGAGACAGGCATCGAGGGC141.1146496815286624No Hit
GTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCCCG141.1146496815286624No Hit
CTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGA120.9554140127388535No Hit
GCCTCAGTCGGCCCACCGGGACGCGATCGCGCGCACCTCCGCGAGACAGG110.8757961783439491No Hit
AGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATC100.7961783439490446No Hit
GGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCA90.7165605095541402No Hit
TTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGGCCCACCGGGACGC90.7165605095541402No Hit
CAGTCGGCCCACCGGGACGCGATCGCGCGCACCTCCGCGAGACAGGCATC90.7165605095541402No Hit
TGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCTC90.7165605095541402No Hit
GGCATCGAGGGCCTCGACGACCCGCGGATCGAAATGGCTGCCGCTGCCGG90.7165605095541402No Hit
ATCCGCGGCCTCAGTCGGCCCACCGGGACGCGATCGCGCGCACCTCCGCG80.6369426751592357No Hit
GCCATTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAG70.5573248407643312No Hit
TGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAG70.5573248407643312No Hit
GGCCGCGGATTATCCGCGGCCTCAGTCGGCCCACCGGGACGCGATCGCGC70.5573248407643312No Hit
AGCGAAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCC70.5573248407643312No Hit
ACCGGGACGCGATCGCGCGCACCTCCGCGAGACAGGCATCGAGGGCCTCG70.5573248407643312No Hit
GGACGCGATCGCGCGCACCTCCGCGAGACAGGCATCGAGGGCCTCGACGA70.5573248407643312No Hit
GCGAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGA70.5573248407643312No Hit
CGGATTATCCGCGGCCTCAGTCGGCCCACCGGGACGCGATCGCGCGCACC70.5573248407643312No Hit
TCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAGGCCGCGGA60.47770700636942676No Hit
ATCGCGCGCACCTCCGCGAGACAGGCATCGAGGGCCTCGACGACCCGCGG60.47770700636942676No Hit
GGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGT60.47770700636942676No Hit
GAGCAAAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGC60.47770700636942676No Hit
GCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCCGCGGGTCGTCGA60.47770700636942676No Hit
ATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGA50.3980891719745223No Hit
CCTCCGCGAGACAGGCATCGAGGGCCTCGACGACCCGCGGATCGAAATGG50.3980891719745223No Hit
CCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCCGCGGGTCGTCGAG50.3980891719745223No Hit
AAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTA50.3980891719745223No Hit
CCGCGAGACAGGCATCGAGGGCCTCGACGACCCGCGGATCGAAATGGCTG50.3980891719745223No Hit
AGACAGGCATCGAGGGCCTCGACGACCCGCGGATCGAAATGGCTGCCGCT50.3980891719745223No Hit
GAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTC40.3184713375796179No Hit
ACGCGATCGCGCGCACCTCCGCGAGACAGGCATCGAGGGCCTCGACGACC40.3184713375796179No Hit
CACCGGGACGCGATCGCGCGCACCTCCGCGAGACAGGCATCGAGGGCCTC40.3184713375796179No Hit
CGATCGCGCGCACCTCCGCGAGACAGGCATCGAGGGCCTCGACGACCCGC40.3184713375796179No Hit
GGCCTCGACGACCCGCGGATCGAAATGGCTGCCGCTGCCGGCCCGGATGA40.3184713375796179No Hit
GAGCAAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCG40.3184713375796179No Hit
GTATATGGGCGTTGGCGGGACTTACGATGTTTTCACCGGTCACGTAAAAC40.3184713375796179No Hit
CCGGGACGCGATCGCGCGCACCTCCGCGAGACAGGCATCGAGGGCCTCGA40.3184713375796179No Hit
GATCGAAATGGCTGCCGCTGCCGGCCCGGATGAAGGCCAGCGCCTCTTCA40.3184713375796179No Hit
GGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGG40.3184713375796179No Hit
TCCGCGGCCTCAGTCGGCCCACCGGGACGCGATCGCGCGCACCTCCGCGA40.3184713375796179No Hit
GCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAGGCCGCGGATA40.3184713375796179No Hit
GAGCGAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCG40.3184713375796179No Hit
GTCTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAGGCCGC40.3184713375796179No Hit
ATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGAT40.3184713375796179No Hit
GCCGCGGATTATCCGCGGCCTCAGTCGGCCCACCGGGACGCGATCGCGCG40.3184713375796179No Hit
AGTCGGCCCACCGGGACGCGATCGCGCGCACCTCCGCGAGACAGGCATCG30.23885350318471338No Hit
CATCGAGGGCCTCGACGACCCGCGGATCGAAATGGCTGCCGCTGCCGGCC30.23885350318471338No Hit
GGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCCGCGGGTCGTCG30.23885350318471338No Hit
GCGGATTATCCGCGGCCTCAGTCGGCCCACCGGGACGCGATCGCGCGCAC30.23885350318471338No Hit
GGATTATCCGCGGCCTCAGTCGGCCCACCGGGACGCGATCGCGCGCACCT30.23885350318471338No Hit
CAGCCATTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGG30.23885350318471338No Hit
AAGCAGGAGATCTTCATGCGCGACTGCCGTCTGGTGCATCCGGATGCGCT30.23885350318471338No Hit
AGCAAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGG30.23885350318471338No Hit
GCGCGCACCTCCGCGAGACAGGCATCGAGGGCCTCGACGACCCGCGGATC30.23885350318471338No Hit
CCATTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGG30.23885350318471338No Hit
AGCGAAAGCAGGCGGTAGAGCCACTCCAGTCCCAGCGTTTGCCAGATTTT30.23885350318471338No Hit
GGTCTAACTGCTTGTTGGAGTTCAGCAGGTAATCATATTCGCCACCCTTG30.23885350318471338No Hit
GCGGCAGCCATTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTC30.23885350318471338No Hit
CGAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGAT30.23885350318471338No Hit
GGCCGGCAGCGGCAGCCATTTCGATCCGCGGGTCGTCGAGGCCCTCGATG30.23885350318471338No Hit
ATCGCGTCCCGGTGGGCCGACTGAGGCCGCGGATAATCCGCGGCCTGATG30.23885350318471338No Hit
GACTGAGGCCGCGGATTATCCGCGGCCTCAGTCGGCCCACCGGGACGCGA30.23885350318471338No Hit
ATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCG30.23885350318471338No Hit
GATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGGC30.23885350318471338No Hit
AGCCACTCCAGTCCCAGCGTTTGCCAGATTTTCGGTGCACGTTTTACGTG20.15923566878980894No Hit
GACTGAGGCCGCGGATAATCCGCGGCCTGATGCAATCCGGCCCCATCCTC20.15923566878980894No Hit
CGACTGCCGTCTGGTGCATCCGGATGCGCTGTATATGGGCGTTGGCGGGA20.15923566878980894No Hit
CGGCAGCCATTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCG20.15923566878980894No Hit
GGCTAAAGATGCAGTGTAGCAAGAGTGGCTAGGTTCTGCTCCTAGACGCT20.15923566878980894No Hit
GGTCAGGGAAAAGGACTTTGACATTGCCAACTTCCTGAACGTACTCGAAC20.15923566878980894No Hit
CCCGCGGATCGAAATGGCTGCCGCTGCCGGCCCGGATGAAGGCCAGCGCC20.15923566878980894No Hit
GAAAGCAGGAGATCTTCATGCGCGACTGCCGTCTGGTGCATCCGGATGCG20.15923566878980894No Hit
CGCGCGCACCTCCGCGAGACAGGCATCGAGGGCCTCGACGACCCGCGGAT20.15923566878980894No Hit
CATATACAGCGCATCCGGATGCACCAGACGGCAGTCGCGCATGAAGATCT20.15923566878980894No Hit
GCGAAAGCAGGCGGTAGAGCCACTCCAGTCCCAGCGTTTGCCAGATTTTC20.15923566878980894No Hit
AAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCA20.15923566878980894No Hit
GTCGGCCCACCGGGACGCGATCGCGCGCACCTCCGCGAGACAGGCATCGA20.15923566878980894No Hit
CCACCGGGACGCGATCGCGCGCACCTCCGCGAGACAGGCATCGAGGGCCT20.15923566878980894No Hit
TCCGCGAGACAGGCATCGAGGGCCTCGACGACCCGCGGATCGAAATGGCT20.15923566878980894No Hit
GCAAAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCG20.15923566878980894No Hit
CTTCCTGAACGTACTCGAACCCTATTACAAGGGTGGCGAATATGATTACC20.15923566878980894No Hit
CTCCAGTCCCAGCGTTTGCCAGATTTTCGGTGCACGTTTTACGTGACCGG20.15923566878980894No Hit
CTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAGGCCGCGG20.15923566878980894No Hit
CTGCCGGCCCGGATGAAGGCCAGCGCCTCTTCATGGCCTGTCTCTTATAC20.15923566878980894No Hit
GCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCG20.15923566878980894No Hit
AGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGC20.15923566878980894No Hit
GGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAGGCCGCGGATAATCCG20.15923566878980894No Hit
GATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGC20.15923566878980894No Hit
GCAAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGA20.15923566878980894No Hit
GCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCCGCGGGT20.15923566878980894No Hit
CTTCATGCGCGACTGCCGTCTGGTGCATCCGGATGCGCTGTATATGGGCG20.15923566878980894No Hit
TCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCC20.15923566878980894No Hit
CGACGACCCGCGGATCGAAATGGCTGCCGCTGCCGGCCCGGATGAAGGCC20.15923566878980894No Hit
CCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGAT20.15923566878980894No Hit
AGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGC20.15923566878980894No Hit
TTCCTGAACGTACTCGAACCCTATTACAAGGGTGGCGAATATGATTACCT20.15923566878980894No Hit
GAAAAGGACTTTGACATTGCCAACTTCCTGAACGTACTCGAACCCTATTA20.15923566878980894No Hit
GAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAGGCCGCGGATAATC20.15923566878980894No Hit
GAAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGC20.15923566878980894No Hit
GAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCC20.15923566878980894No Hit
ATTACAAGGGTGGCGAATATGATTACCTGCTGAACTCCAACAAGCAGTTA20.15923566878980894No Hit
CCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCG20.15923566878980894No Hit
GCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGGCCCACCG20.15923566878980894No Hit
AGCCATTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGA20.15923566878980894No Hit
GATTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGGCCCCCCGGGAC20.15923566878980894No Hit
TCGAGGGCCTCGACGACCCGCGGATCGAAATGGCTGCCGCTGCCGGCCCG20.15923566878980894No Hit
TCGAGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCCCGG20.15923566878980894No Hit
CACCTCCGCGAGACAGGCATCGAGGGCCTCGACGACCCGCGGATCGAAAT20.15923566878980894No Hit
CAGGAGATCTTCATGCGCGACTGCCGTCTGGTGCATCCGGATGCGCTGTA20.15923566878980894No Hit
GTTGAGGATGGGGCCGGATTGCGTCAGGCCGCGGATTATCCGCGGCCTCA20.15923566878980894No Hit
GGATTGCATCAGGCCGCGGATAATCCGCGGCCTCAGTCGGCCCACCGGGA20.15923566878980894No Hit
CGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGC20.15923566878980894No Hit
AGGCATCGAGGGCCTCGACGACCCGCGGATCGAAATGGCTGCCGCTGCCG20.15923566878980894No Hit
GTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTC20.15923566878980894No Hit
TCTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAGGCCGCG20.15923566878980894No Hit
GACAGGCATCGAGGGCCTCGACGACCCGCGGATCGAAATGGCTGCCGCTG20.15923566878980894No Hit
AGCAAAAGCAGGTCAGGGAAAAGGACTTTGACATTGCCAACTTCCTGAAC20.15923566878980894No Hit
AAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCCTGTCTCTTATACA20.15923566878980894No Hit
ATCGAGGGCCTCGACGACCCGCGGATCGAAATGGCTGCCGCTGCCGGCCC20.15923566878980894No Hit
GAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCTCTGTC20.15923566878980894No Hit
GCATCGAGGGCCTCGACGACCCGCGGATCGAAATGGCTGCCGCTGCCGGC20.15923566878980894No Hit
GGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCCTGTCTCTTATACAC20.15923566878980894No Hit
AGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGG20.15923566878980894No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)