FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_G05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_G05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842066
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT89680.48684466246051983No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA78830.42794340702233247No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT78280.4249576290968945No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG68210.37029074962569203No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT58320.3166010338391784No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC57520.31225808412945033No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA51200.2779487814225983No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT45920.24928531333839288No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG44120.23951367649150465No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA41100.22311904133728108No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC41050.22284760698042305No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA40710.22100185335378864No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT35640.1934784095683868No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC35550.19298982772604237No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT34850.1891897467300303No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA33810.18354391210738377No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC31920.17328369341815114No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG31650.17181794789111793No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30690.16660640823944417No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT30040.1630777616002901No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTCCATGGTGTATCCT29120.1580833694341028No Hit
GGATACACCATGGACATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGA27950.15173180548362547No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG27940.15167751861225384No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG27420.1488546013009306No Hit
GGATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC27150.14738885577389735No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC27120.14722599515978255No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT26640.1446202253339457No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC25890.1405487099810756No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG25490.13837723512621156No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC25030.1358800390431179No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT24450.13273140050356502No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC24360.1322428186612206No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT23840.12941990134989734No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA22810.12382835359862242No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTC22800.1237740667272508No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA22680.1231226242707916No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT21960.11921396953203632No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT21740.11801965836186108No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG21550.11698820780580066No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG21340.11584818350699702No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA21230.11525102792190943No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG20970.11383956926624779No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA20620.11193952876824174No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG20330.1103652094984653No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT19710.10699942347342603No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT19600.10640226788833841No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC19560.10618512040285201No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC19510.10591368604599401No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG19410.10537081733227799No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA19370.10515366984679159No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT19370.10515366984679159No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT19150.10395935867661635No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC18960.10292790812055594No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG18880.10249361314958313No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT18810.10211360504998192No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA18580.10086500700843509No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA18460.10021356455197589No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)