FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_F06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_F06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707577
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT39600.23190755087471895No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG34190.20022523142441015No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA33990.1990539811674671No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA32380.18962541659907575No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA29110.1704754748980573No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG27350.1601684726369587No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT26730.1565375968404353No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA26360.1543707838650907No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA26050.15255534596682901No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA25220.14769465740051546No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT23430.1372119676008754No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA23420.13715340508802823No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT22320.1307115286748416No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA22260.13036015359775868No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG21980.12872040323803846No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC21440.1255580275442923No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG20990.12292271446617048No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC20940.12262990190193472No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC20630.12081446400367304No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC20450.11976033877242431No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA19780.11583665041166519No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG19700.11536815030888797No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC19680.11525102528319367No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC19370.11343558738493198No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG19320.11314277482069622No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC18760.10986327410125575No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT18620.10904339892139564No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA17120.10025902199432295No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC17110.1002004594814758No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)