FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_F01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_F01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279808
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA50650.2221678316770535No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT42880.18808601426085003No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA30010.13163389197686823No Hit
ATATTATGGTAGTACAAATATACTCTGCGGAAGGAAAGTTAAAAGCAAGT25230.11066721408118578No Hit
CAAATATACTCTGCGGAAGGAAAGTTAAAAGCAAGTATTTAGCCTCTATT25110.11014085396664983No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC24250.1063686064791421No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)