FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39207
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCA15704.004386971714235No Hit
GGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAG15273.894712678858367No Hit
GTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAG11132.8387787895018746No Hit
GAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTAC10072.56841890478741No Hit
CCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCG8582.1883847272170787No Hit
GACTAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTT8382.1373734282143495No Hit
GTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGG7311.8644629785497489No Hit
AGCGAAAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTAC7161.826204504297702No Hit
TCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGA6951.7726426403448365No Hit
AGCGAAAGCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGC6661.698676256790879No Hit
GAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGG6431.6400132629377406No Hit
GTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGATGC6161.5711480092840564No Hit
GCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGG5731.4614737164281888No Hit
AAAGCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGA4961.2650802152676819No Hit
GGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTA4911.2523273905169996No Hit
TGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGAC4701.198765526564134No Hit
ACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGT4241.081439538857857No Hit
GGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACGAGCATTG3931.002372025403627No Hit
CCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCC3870.9870686357028082No Hit
TGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGC3850.9819675058025352No Hit
AGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACGAGCATT3820.9743158109521258No Hit
CTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGG3590.9156528170989874No Hit
GCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCTT3360.8569898232458489No Hit
AAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGG3220.8212819139439385No Hit
GTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTG3070.7830234396918918No Hit
AAAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTG2960.7549672252403907No Hit
AAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGAT2920.7447649654398449No Hit
CGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAA2890.7371132705894355No Hit
GGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAG2880.7345627056392992No Hit
GTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAA2870.7320121406891626No Hit
GATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGG2790.7116076210880711No Hit
AGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGA2660.6784502767362971No Hit
AGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGATG2640.6733491468360242No Hit
AGCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGC2520.6427423674343867No Hit
GGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACG2510.6401918024842502No Hit
GCGAAAGCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCT2440.622337847833295No Hit
CTCCTGTACTAGTCCCTGCTGCGTCAGCCTACCCTACTGCCAGAGTAGTC2410.6146861529828856No Hit
GCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACT2400.6121355880327493No Hit
GGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCT2330.5942816333817941No Hit
AGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAG2280.5815288086311118No Hit
GACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAA2230.5687759838804295No Hit
CTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTT2190.5585737240798837No Hit
GTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGC2170.5534725941796108No Hit
GGCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAA2160.5509220292294743No Hit
CAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACGAG2140.5458208993292014No Hit
CTAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCC2130.5432703343790649No Hit
TCTTGAAGCAATGCTCGTTAACCCCTCCTGTACTAGTCCCTGCTGCGTCA2100.5356186395286556No Hit
GCGAAAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACT2030.5177646848777003No Hit
GAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTG2010.5126635549774274No Hit
GGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGA2000.510112990027291No Hit
GCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGA1990.5075624250771545No Hit
GTACTAGTCCCTGCTGCGTCAGCCTACCCTACTGCCAGAGTAGTCTCTTC1960.49991073022674526No Hit
GCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTG1870.4769556456755171No Hit
GGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACGAGCATTGCTTCA1870.4769556456755171No Hit
ATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGC1840.4693039508251078No Hit
GCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCT1810.4616522559746984No Hit
CTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGG1780.45400056112428905No Hit
CAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTC1640.4182926518223787No Hit
GAGCGAAAGCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTG1630.41574208687224223No Hit
GTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACGAGCA1470.3749330476700589No Hit
GCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAAT1390.3545285280689673No Hit
CAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAA1370.34942739816869434No Hit
CTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGCT1360.3468768332185579No Hit
CTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCC1330.33922513836814855No Hit
CAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGT1330.33922513836814855No Hit
CAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGC1310.33412400846787566No Hit
TTCCTAGCATCTGCAGGGACACAAGGGCCTTTGCTTCCGGGCCACTTGGC1280.3264723136174663No Hit
GTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAG1280.3264723136174663No Hit
AACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGGACA1270.3239217486673298No Hit
TGCTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAACCCCTCCTGT1260.32137118371719336No Hit
AAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGG1210.3086183589665111No Hit
CTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAACCC1200.30606779401637463No Hit
ACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGC1200.30606779401637463No Hit
GCAGGGACTAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGA1200.30606779401637463No Hit
GTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTA1170.29841609916596523No Hit
GCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAA1160.2958655342158288No Hit
CCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATG1150.29331496926569234No Hit
GCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCC1150.29331496926569234No Hit
GAGCGAAAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTA1130.28821383936541944No Hit
ACGCAGCAGGGACTAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGG1130.28821383936541944No Hit
CTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAA1080.27546101461473715No Hit
AGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTA1070.2729104496646007No Hit
CAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAG1060.27035988471446426No Hit
TAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGC1060.27035988471446426No Hit
CAGCAGGGACTAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGA1050.2678093197643278No Hit
CACAAGGGCCTTTGCTTCCGGGCCACTTGGCCTTAACTTTGTTCCTGAGC1030.2627081898640549No Hit
TCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAA1030.2627081898640549No Hit
GGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCTTTGCTTCCGG1030.2627081898640549No Hit
CTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGT1010.25760705996378197No Hit
GTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTA1000.2550564950136455No Hit
GCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCA980.24995536511337263No Hit
GCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGG940.2397531053128268No Hit
TTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCTTTG930.23720254036269034No Hit
CATCTGCAGGGACACAAGGGCCTTTGCTTCCGGGCCACTTGGCCTTAACT920.2346519754125539No Hit
AGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACA920.2346519754125539No Hit
GTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGG900.22955084551228097No Hit
GAAGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCT890.22700028056214452No Hit
CTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCTTT890.22700028056214452No Hit
CGTTAACCCCTCCTGTACTAGTCCCTGCTGCGTCAGCCTACCCTACTGCC890.22700028056214452No Hit
CAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCC870.22189915066187163No Hit
GCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCA850.21679802076159868No Hit
GCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTA830.2116968908613258No Hit
ACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAA830.2116968908613258No Hit
CCTCTTGAAGCAATGCTCGTTAACCCCTCCTGTACTAGTCCCTGCTGCGT820.20914632591118934No Hit
GGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAG810.2065957609610529No Hit
CTTGAAGCAATGCTCGTTAACCCCTCCTGTACTAGTCCCTGCTGCGTCAG810.2065957609610529No Hit
AGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAG810.2065957609610529No Hit
GAAAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCT790.20149463106077994No Hit
CTGCAGGGACACAAGGGCCTTTGCTTCCGGGCCACTTGGCCTTAACTTTG790.20149463106077994No Hit
CTCTCACCTCTTGAAGCAATGCTCGTTAACCCCTCCTGTACTAGTCCCTG780.1989440661106435No Hit
AGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCA770.19639350116050705No Hit
GACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAG760.1938429362103706No Hit
GGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGG760.1938429362103706No Hit
AGGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCTTTGCTTCCG750.19129237126023416No Hit
GAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGC750.19129237126023416No Hit
GAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCTTTGCTTCCGGGCCAC740.18874180631009768No Hit
AGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAACCCCTCCTGTACTA730.18619124135996123No Hit
GCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACGA730.18619124135996123No Hit
GTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGT700.17853954650955187No Hit
GGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCATCT700.17853954650955187No Hit
CGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTA690.1759889815594154No Hit
TAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCT690.1759889815594154No Hit
AGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCC680.17343841660927894No Hit
ACTCTCACCTCTTGAAGCAATGCTCGTTAACCCCTCCTGTACTAGTCCCT670.1708878516591425No Hit
GGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAA650.1657867217588696No Hit
CAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCAT640.16323615680873316No Hit
GGACTAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTT610.15558446195832376No Hit
GACACAAGGGCCTTTGCTTCCGGGCCACTTGGCCTTAACTTTGTTCCTGA610.15558446195832376No Hit
CGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTG600.15303389700818731No Hit
CAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAAAA570.14538220215777795No Hit
GGCCTTTGCTTCCGGGCCACTTGGCCTTAACTTTGTTCCTGAGCTCTAGC560.14283163720764147No Hit
GAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTG540.13773050730736858No Hit
GGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAACCCCTCCTGTACTAG520.13262937740709568No Hit
GAAAGCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAG520.13262937740709568No Hit
GGACACAAGGGCCTTTGCTTCCGGGCCACTTGGCCTTAACTTTGTTCCTG500.12752824750682276No Hit
TTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGCTC480.12242711760654984No Hit
CTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAACCCCTCCTGTAC480.12242711760654984No Hit
AGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGT470.1198765526564134No Hit
GCAGGGACACAAGGGCCTTTGCTTCCGGGCCACTTGGCCTTAACTTTGTT450.11477542275614049No Hit
TGCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCT440.11222485780600404No Hit
CGAAAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTC440.11222485780600404No Hit
AGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGC430.10967429285586756No Hit
CGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGAC420.10712372790573112No Hit
GAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGG420.10712372790573112No Hit
AGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTG420.10712372790573112No Hit
GTTTTCCTAGCATCTGCAGGGACACAAGGGCCTTTGCTTCCGGGCCACTT420.10712372790573112No Hit
TACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGG410.10457316295559467No Hit
ACACAAGGGCCTTTGCTTCCGGGCCACTTGGCCTTAACTTTGTTCCTGAG410.10457316295559467No Hit
GACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACG410.10457316295559467No Hit
CTCTTGAAGCAATGCTCGTTAACCCCTCCTGTACTAGTCCCTGCTGCGTC410.10457316295559467No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)