FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_D08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_D08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608472
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTGATAGTATGTGTCTTTTCATTGGTACATTTAAACCATTGTTGCAAA116150.7221139068631596No Hit
ATCTAATAGATAGATAACATTCTAAGAAAGGAGCAAAATTAGAATCATAT65870.40951909638464334No Hit
ATCACACACTGCGCATCGCGCATTCTATAATTTCTATTCATTGAAGGATG56000.3481565112728105No Hit
GATAGTATGTGTCTTTTCATTGGTACATTTAAACCATTGTTGCAAAAAAA41740.2595009425094127No Hit
ACACATACGTATCTAACAGATAAATCTAATAGATAGATAACATTCTAAGA40350.25085920053317684No Hit
CTCTAAACCCATTCAACCATTCAGCCCTTTGCTTTCTGATAAGACTGCCA40010.24874539314330618No Hit
CATATAAAATGCTCAGCTAAAACCACAAAAGGCAAAAAGAAAATGGAAGA38760.2409740424452524No Hit
GGCTAAACCTGCTCTCCTTCCAAAAATAAAAGAAATCCTCCGTAGAAACA38380.23861155183304406No Hit
ATAGATAACATTCTAAGAAAGGAGCAAAATTAGAATCATATAAAATGCTC35610.22139023868615682No Hit
GTTTAAAATATAATGGCTAAACCTGCTCTCCTTCCAAAAATAAAAGAAAT34260.2129971799322587No Hit
CTAATAGATAGATAACATTCTAAGAAAGGAGCAAAATTAGAATCATATAA31710.19714362450822892No Hit
CTTCTGTAGCGTGTGCACCTGCCTACCAGTTAGCTTAGTGGGCAGAAAGA31300.1945946214792673No Hit
AATATATACATATGACACATACGTATCTAACAGATAAATCTAATAGATAG30970.1925429848949811No Hit
CTATAATTTCTATTCATTGAAGGATGAGGTCAGAATGAAGTGTTCCCGTT30470.18943444461575956No Hit
GTCTCAGGACCCTGGGATCATGAGCTAAGCCAGAGGCAGGTGCTTAACCA30160.18750714964264223No Hit
ATTTAAACCATTGTTGCAAAAAAAAAATGCTTATTGATGTAATTGAACTT28610.1778706747770555No Hit
GTATGTGTCTTTTCATTGGTACATTTAAACCATTGTTGCAAAAAAAAAAT28340.17619206302627585No Hit
GTCTATAGTTTACTCAGTAATGCCCCCAAAGAAAGAAACTAATGTCTCTT28010.17414042644198965No Hit
TCTGTAGCGTGTGCACCTGCCTACCAGTTAGCTTAGTGGGCAGAAAGAAG26700.1659960509104293No Hit
ATAGTAACCAATGTGGTAGATAGAAGTTCAATTACATCAATAAGCATTTT26220.16301185224237663No Hit
GCTAAAACCACAAAAGGCAAAAAGAAAATGGAAGACAAAATAGGAACAAA26030.16183060693627244No Hit
AGCAGGAGGAGTGGGAGAGGGAGAAGCAGGCTTCCCACTGAGCAGGGAGC25690.1597167995464018No Hit
CATTCTAAGAAAGGAGCAAAATTAGAATCATATAAAATGCTCAGCTAAAA24990.15536484315549168No Hit
ACATATGACACATACGTATCTAACAGATAAATCTAATAGATAGATAACAT23610.14678527198484026No Hit
CTCCTATATTTATTATAGATTCGATTCTCCCTAGTTATGAAACTTAAGGT23130.1438010733167876No Hit
GCCAACAACTATGCCAATTAGTGTCAATTGCGATGTTCGCTTCTGTAGCG22910.14243331559393016No Hit
GAGTGGGAGAGGGAGAAGCAGGCTTCCCACTGAGCAGGGAGCCCAATGAG22750.14143858270457926No Hit
GATAACATTCTAAGAAAGGAGCAAAATTAGAATCATATAAAATGCTCAGC22200.13801918839743557No Hit
GTAATCACACACTGCGCATCGCGCATTCTATAATTTCTATTCATTGAAGG21960.13652708906340927No Hit
GTCTTAGGCCACCCCCTGGGCTGAGAGGAAGCAGGAGTGTGGACAGAAAG21510.13372940281210988No Hit
CATATGACACATACGTATCTAACAGATAAATCTAATAGATAGATAACATT21030.13074520414405721No Hit
GTAAAAGGTTACCTGAAAAATAATCAGTAATCACACACTGCGCATCGCGC20920.13006132528262848No Hit
CTACAGAAGCGAACATCGCAATTGACACTAATTGGCATAGTTGTTGGCAG20360.12657976016990036No Hit
CCTATATTTATTATAGATTCGATTCTCCCTAGTTATGAAACTTAAGGTTT20070.1247768068079519No Hit
ATTTATTATAGATTCGATTCTCCCTAGTTATGAAACTTAAGGTTTTTAAA19900.12371990311301657No Hit
GTATCTAACAGATAAATCTAATAGATAGATAACATTCTAAGAAAGGAGCA19840.12334687827950999No Hit
GTTCTAGAGGCTGAAAGTCCAAGCGAGCTCAAGGTGCTGGCAGGGCTGGT19660.12222780377899026No Hit
GGTTACTATTTTCTATTTCTTGCTATTGTTCTTTGTTCCTATTTTGTCTT19260.11974097155561304No Hit
GATAAATCTAATAGATAGATAACATTCTAAGAAAGGAGCAAAATTAGAAT18180.11302652455249455No Hit
CATCTATGGTGACTGTAGGGTCTTGTTACCATAGTCTGCTTTTATGGATT18140.11277784133015682No Hit
CATTTAAACCATTGTTGCAAAAAAAAAATGCTTATTGATGTAATTGAACT18100.1125291581078191No Hit
AAATTAGAATCATATAAAATGCTCAGCTAAAACCACAAAAGGCAAAAAGA17880.11116140038496163No Hit
GAGCAGGGAGCCCAATGAGGGACTCAGTCTCAGGACCCTGGGATCATGAG17770.1104775215235329No Hit
CCCTAGTTATGAAACTTAAGGTTTTTAAAAAAGTGTTACTGTTACAGTTT17710.1101044966900263No Hit
GGCTTCCCACTGAGCAGGGAGCCCAATGAGGGACTCAGTCTCAGGACCCT17160.10668510238288265No Hit
AATTAGAATCATATAAAATGCTCAGCTAAAACCACAAAAGGCAAAAAGAA16850.10475780740976529No Hit
GTGCTTAACCAACTGAACCACCCAGGCACCCCCTTTTTTAATCTTTTTTA16610.10326570807573897No Hit
CCCAAGAAGAGTATCCTAGGCTTTACTTACTAGATTCCAATAGCACCTCA16610.10326570807573897No Hit
CTGTTACAGTTTAAAATATAATGGCTAAACCTGCTCTCCTTCCAAAAATA16570.10301702485340124No Hit
CTATCAGAATGGTCAATAAAAAAGATTAAAAAAGGGGGTGCCTGGGTGGT16180.10059236343560846No Hit
CCTATATTCATTATTGTGAAGAATCTTTACACTGCCTTTCTAGAGAGTGG16120.10021933860210187No Hit
GAACAATAGCAAGAAATAGAAAATAGTAACCAATGTGGTAGATAGAAGTT16090.10003282618534858No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)