FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_D06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_D06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972873
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG43350.21973031208800567No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT38700.19616062463219883No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC37190.18850681214654974No Hit
TTCCTATACAGATGGCTCTAATTTGGGAAGTACACTGACTTTTTGACTTG36490.18495868715320246No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC34550.17512531217164004No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA33100.1677756246854207No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA29060.14729787472381647No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA28660.14527037472761806No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA28020.14202637473370053No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT27540.13959337473826242No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC25100.12722562476145197No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG24290.12311993726915012No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC24020.12175137477171617No Hit
GGCTAATAGAAGGTAGAAAGTCAATTAGAGACGATTACAAACTAACTGAC23890.12109243727295169No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA23690.12007868727485246No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT23450.1188621872771334No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA22040.1117152497905339No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG21920.11110699979167438No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20800.10542999980231876No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC20740.10512587480288899No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT20090.10183118730906654No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG19910.10091881231077723No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC19810.10041193731172762No Hit
GTTCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACA19770.10020918731210778No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)