FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_D04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_D04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915618
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA79230.41360020630417965No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA65910.34406651012884615No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT60930.3180696777750053No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT53080.27709073520921185No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG49920.2605947532336823No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC49160.2566273651636182No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC48490.2531297993650091No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG47740.24921461376955115No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC43990.2296386857922613No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG42690.22285236409346748No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA39130.2042682831336937No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC37930.19800398618096093No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA35980.1878245036327702No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC34510.1801507398656726No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA33230.173468823116091No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA29330.15310985801970958No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT29090.15185699862916302No Hit
CATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGATATGT27880.14554049920182416No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT27780.1450184744557631No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA27220.14209513587782116No Hit
ATATATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAAT26970.1407900740126685No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT26750.13964161957133414No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC26700.13938060719830364No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA26160.1365616735695739No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA25680.13405595478848079No Hit
AAATTACACTGGCCATTCACCAGAAAAAAAATGAACTCAAGCCCAACCAT24440.1275828479373236No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA24140.12601677369914044No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG22960.1198568816956199No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA22520.11755997281295122No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG22370.11677693569385962No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG22110.11541967135410088No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT21580.11265294019997725No Hit
GTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGCCAGTGTAA21350.11145228328403681No Hit
GGCCACATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGA21330.11134787833482458No Hit
CAATAAATATATTTAATAAAAATAGATTAACCAGGGCAACTAGGTGGCTC21070.10999061399506582No Hit
GTTTATTTATTTGACAGAGAGAGAAGGAAAGAGAGCAAACAGGGGGAGCA20910.10915537440136813No Hit
GAAGGAAAGAGAGCAAACAGGGGGAGCAACAGGCAGAGGGAGAGGGAGAA20630.10769370511239715No Hit
CTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTA20460.10680626304409334No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA19860.10367411456772696No Hit
TCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTT19700.10283887497402927No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA19470.10163821805808883No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT19370.10111619331202777No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)