FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_C09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_C09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810261
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC70110.38729221918828277No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG66490.36729510275037686No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT62060.34282349340785667No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA58110.32100343541621895No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT54570.30144824420346017No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA40890.22587903070330745No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC40490.22366940457757195No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT34580.19102217856983053No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC33940.1874867767686538No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG30300.16737917902446112No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG29440.16262848285412987No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT29150.16102650391297166No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT29060.16052933803468117No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC28870.15947976562495683No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT28290.15627580774264044No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC27830.15373473769804466No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT25860.1428523290287975No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG24300.1342347871384292No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT24300.1342347871384292No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA23390.12920788770238104No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT23270.1285449998646604No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG23060.1273849461486493No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA21220.11722066597026617No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA21080.11644729682625875No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT21080.11644729682625875No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA21030.11617109356054182No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA20170.11142039739021059No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC19910.10998414040848253No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA19790.1093212525707619No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT19510.10777451428274708No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA19400.10716686709816982No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAA19350.1068906638324529No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA18870.10423911248157033No Hit
TTTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTT18770.10368670595013647No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAG18740.10352098399070632No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC18510.10225044896840843No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC18480.10208472700897826No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG18380.1015323204775444No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)