FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_C05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_C05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009950
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC75990.37806910619667156No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG63680.31682380158710416No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA38770.19289037040722407No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA35510.17667106146919076No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT31620.15731734620264187No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA31540.15691932635140177No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA31280.15562576183487153No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA31170.15507848453941642No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC30930.15388442498569616No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC29750.14801363217990499No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT28770.14313788900221397No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT28230.14045125500634345No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA27430.13647105649394262No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA27080.1347297196447673No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT26960.13413268986790716No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC26390.1312967984278216No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT25080.12477922336376526No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT24820.12348565884723502No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG24460.12169456951665464No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT24010.11945570785342918No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT23300.11592328167367347No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA22980.11433120226871316No Hit
GTCTAGAGCTGAGAACAGGATTGGGTATTCTAAATCCTTTTATCTCTGTT22310.11099778601457748No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG21700.10796288464887185No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG21670.10781362720465684No Hit
GTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATA21600.10746535983482176No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG21540.1071668449463917No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT21390.10642055772531656No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC21350.10622154779969652No Hit
GTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAAAA21200.10547526057862136No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT21040.10467922087614119No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC20150.1002512500310953No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)