FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_B05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_B05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1652623
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG77460.4687094394789374No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA54260.3283265451346133No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT44190.2673931078049864No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT43340.26224976900357794No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA43120.26091855190203694No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC42400.2565618413879027No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA40910.24754587101837502No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG39270.23762225262506936No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT38450.23266044342841655No Hit
GTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATA37320.22582282831595588No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA37200.22509670989693353No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT36650.22176866714308102No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT34650.20966669349270825No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC31110.18824620013154844No Hit
GTCTATATGGCACCAACTTTCTTCCACCATTTTCTTTAGTTTCAGTGCCT27740.16785437453067034No Hit
ATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTC27260.16494990085458086No Hit
TTTTAATACATCAGGCATTTTTCTCAATGACTTCAGATAGGTAAGTCTCC27100.16398174296255105No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT24880.15054855221063726No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA24670.1492778449773481No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC23930.14480011472671023No Hit
ATATCACACTGTATGTTAATTACACTGGAATTAAAAACAAAGATATGCAT23900.14461858512195463No Hit
AATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGA23460.14195615091887262No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT23040.13941473645229432No Hit
GTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATA22280.13481598646515267No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG22080.1336057891001154No Hit
ATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATC20830.1260420555686324No Hit
TATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACCTG20160.12198789439575752No Hit
GATATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACC20090.12156432531799449No Hit
AGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGTGAAATTAAACTC19900.12041463782120906No Hit
GTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCT19840.12005157861169789No Hit
GCAGAGGGAGAGGAGGAAGCAGGCTCCCCACTCAGCAGGGAGCCCAATGT19770.11962800953393485No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT19590.11853883190540129No Hit
CTCATGAGCAAACCGCACATCAGAGGAATTAAAAGATGACCTGATGGAGA19400.11738914440861588No Hit
ATAAAAAAGAGTGCATGTTATAAAAGAAGTCAAAGGCGGTCTTGAATAAG19290.11672353585784538No Hit
ATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGT18800.11375855231350404No Hit
GAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAA18720.11327447336748914No Hit
CTGTAAGCTTTGAACTACTGACTTGGACATCATTCATGGAGACTTACCTA18420.11145917731993323No Hit
ATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAA18220.11024897995489595No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG17770.10752603588356208No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA17290.1046215622074726No Hit
ATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCATTC17220.10419799312970957No Hit
GCCCAATGTGGGACTCGATCCCAGGACCCTGAGATCATGACCTGAGCCGA16760.10141453919012382No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA16620.10056740103459774No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)