FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_B02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_B02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436647
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAACTCACTCTTACATGATCTCATTTAATCTTCATAACAATGTCAG27740.1930884900744581No Hit
GTGTATACTCCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAG23610.16434099677930628No Hit
ATGTAAGAGTGAGTTAGTACCATGCCTAGCCCAAAGAAGTAGCTATAGAA20360.14171887735818192No Hit
GTCTTAGGGACAATAACTTTGTGTCACCATTTGAAAATCTCAAATTCTAT19390.1349670447924925No Hit
ACATTAGGGATTCATAGGCCATAAGGAGTAGTTTGGATTTTATTCTAAGT18260.12710150788607083No Hit
GTCAAAGGATGTGCTGATACTGCCTTCCAGAAAGTTCATGTCAGTTCCAT18190.12661426223700045No Hit
GTGTAGGGGCTCCCAGGCACGGGGTGTCCATCGTAGGTGACGTCGACGGC17470.12160259270370523No Hit
CTCTTACATGATCTCATTTAATCTTCATAACAATGTCAGGAATCTAAAAT17330.12062810140556449No Hit
AGTTAGTACCATGCCTAGCCCAAAGAAGTAGCTATAGAAACGTCAGTTTC16840.11721738186207188No Hit
GAGTTAGTACCATGCCTAGCCCAAAGAAGTAGCTATAGAAACGTCAGTTT16220.11290177754173433No Hit
GAAATAAACAGTTGATTTAACATAAATTCAAGACGTCCAAAAAGATGATG16140.11234492537136818No Hit
CATTTGGAAAGATGTCACTATCGCCAACTTAACAGGTTACTCTTAACGTT14980.1042705689010592No Hit
GTGTAGACAGTCCCTTCTCTTGTCCCCCGCTCTGAACTACAAGTTTCCTG14770.10280883195384809No Hit
GTGTAGACTGGGATTGATGCCTACATGTCTTAGGGACAATAACTTTGTGT14550.10127748848534122No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)