FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_A10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_A10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581387
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT124210.7854497349478654No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA77740.49159377179653047No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA75980.480464301274767No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC72500.45845830274309834No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG71540.45238768245850003No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA62310.3940211978472063No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA57240.3619607344691717No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC52370.3311649836504284No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG48560.307072209395929No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA43030.2721029071315244No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG40750.2576851839556035No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG37890.23959979435773784No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT37680.238271846170482No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG37320.23599536356375767No Hit
CTATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGT36910.23340270281721046No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT36520.23093651332659243No Hit
GATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAA34980.22119822662004934No Hit
CTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAA34100.2156334913591676No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG32350.20456725646536866No Hit
GAGCAAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCA30750.19444955599103825No Hit
ACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGA30620.1936274928274989No Hit
ATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGTAA30550.19318484343174694No Hit
ATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAAC30240.19122453896484543No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG29500.18654510249546757No Hit
GTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAA28450.17990536155918824No Hit
AAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTC28180.17819799960414498No Hit
ACCCAAATGTGCTCTGATGATGAATGGATAAACAAAACATGGTGTATACA28020.17718622955671193No Hit
GTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCT25530.16144055819353517No Hit
GGTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATC25530.16144055819353517No Hit
GTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATA24310.15372581158185822No Hit
CTTCTAATTGTGGTAAAATACAATAACACAAAACTCACACCTTAATCATT22780.14405076050327972No Hit
CAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTA22470.14209045603637818No Hit
GATGATGAATGGATAAACAAAACATGGTGTATACATACAATGGCATATTA22340.14126839287283882No Hit
CTCTAAGTACCTTATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAA22320.1411419216169097No Hit
GTTCTAATAGTTACCCTAGCTAGAAACCTCAAAACATTTTTAGTTCCTTC20960.13254187621372882No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG20220.12786243974435102No Hit
ACCTAATATAGTAGCATTTATAGGCAATGGTGCTAATAGTGAGAGTTTGT20100.12710361220877622No Hit
ACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTT19400.12267711825125664No Hit
CCTCCAAATAGCTAAAAGGTTAATCAACCCAAATGTGCTCTGATGATGAA19150.12109622755214253No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA19130.1209697562962134No Hit
CCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCC18810.11894621620134731No Hit
GGCTACAGCATGGATGAACTTGGAGATCATATGTGAAGTAAAATAAGCTA18770.11869327368948902No Hit
GTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATC18770.11869327368948902No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA18390.11629031982683556No Hit
TTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTCTGTTGTCCT18040.11407707284807576No Hit
ATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTA18000.11382413033621751No Hit
GTAAAATACAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACG17900.11319177405657187No Hit
GTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAG17430.1102196995422373No Hit
ACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACA17190.10870204447108772No Hit
GCTAAAAGGTTAATCAACCCAAATGTGCTCTGATGATGAATGGATAAACA16350.10339025172206423No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA15920.10067111971958793No Hit
GTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTCTGTTGTCCTTG15850.10022847032383597No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)