FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_A08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_A08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353500
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT55770.2369662205226259No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA40020.17004461440407903No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG34230.1454429572976418No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC33780.1435309114085405No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA32350.13745485447206288No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA31620.13435309114085406No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA27260.11582749097089441No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC26780.11378797535585299No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT24790.1053324835351604No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)