FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_A04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_A04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1653982
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC77380.46784064155474486No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT45360.2742472409010497No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA44680.2701359506935384No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT39470.23863621248598837No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG38010.2298090305698611No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA36480.2205586276029606No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC33660.2035088652718107No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA33000.1995184953645203No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC32870.1987325134130843No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT32250.19498398410623574No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA31880.1927469585521487No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT29540.17859928342630088No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG28750.17382293156757447No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC27960.16904657970884812No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG27190.16439114815034261No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA25920.15671270908631413No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT25790.15592672713487812No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG25580.15465706398255846No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA25320.15308510007968648No Hit
ATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGTAAA25170.15217819782802955No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA24840.15018301287438438No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG24250.14661586401786716No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT23150.13996524750571648No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT22370.13524935579710057No Hit
GTCTAGAGCTGAGAACAGGATTGGGTATTCTAAATCCTTTTATCTCTGTT22200.13422153324522273No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC22010.13307279039312397No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC21250.12847781898472896No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG20810.1258175723798687No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA20670.12497113027832224No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC20520.12406422802666535No Hit
GTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAAAA20440.12358054682578166No Hit
ATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGC20030.12110168067125278No Hit
GTTAAAAGTGTGATGTTGCTATCTAGTATATAAGCATGCAAATCCATGAA19360.11705085061385191No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA18930.1144510641591021No Hit
ATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGT18350.11094437545269538No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA18290.11058161455203262No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA18190.109977013050928No Hit
CATAAAAAGTTATTCTATTATTCATGCTTGTATTCTGGACTGAGATTAGT17520.10592618299352713No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC17280.10447513939087609No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC16990.10272179503767272No Hit
ATTCTGGACTGAGATTAGTCTAGAGCTGAGAACAGGATTGGGTATTCTAA16760.10133121158513213No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA16720.10108937098469027No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)