FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837428
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT93140.506904216110781No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG81200.4419220780351666No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG74240.4040430427750094No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT73630.4007231847996221No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA68070.3704634957124851No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG60150.32735976593368554No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA58960.3208833216866185No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC57370.31222992139011707No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA52120.2836573732412916No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA46640.2538330753640415No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC46140.25111188030224857No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT41250.22449859259791402No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT40570.22079776731387568No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC40260.2191106263755641No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG35830.19500083812807903No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC34850.18966729580696495No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT33900.18449702518955846No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA33620.18297315595495442No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA33530.18248334084383172No Hit
ATATATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAAT32970.17943560237462367No Hit
CATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGATATGT32930.17921790676968022No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA30380.16533981195453645No Hit
TCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTT27790.15124402153444924No Hit
CTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTA27660.1505365108183831No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC27570.15004669570726037No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA27480.14955688059613764No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG27370.1489582176825432No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT27160.14781531575659018No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA27030.14710780504052404No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA26950.14667241383063717No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG26950.14667241383063717No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA26780.14574720750962758No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA25660.1396517305712115No Hit
TTATTTCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATG25480.13867210034896607No Hit
AACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAA25390.13818228523784332No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT24990.13600532918840902No Hit
GATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCC24000.13061736296605908No Hit
ATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATA23390.12729750499067174No Hit
GACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGA22670.12337898410168997No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA21920.11929719150900063No Hit
ATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGT21730.11826313738551933No Hit
GGCCACATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGA21210.11543309452125472No Hit
ACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAAC21210.11543309452125472No Hit
ATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGT21150.11510655111383956No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG21120.11494327941013198No Hit
CTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGA20860.11352825797799969No Hit
TCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGAC19720.10732393323711188No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC19660.10699738982969673No Hit
CTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCA19450.1058544879037437No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA18820.10242578212588466No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA18690.1017182714098185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)