FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_A02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_A02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486952
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGCACCTGGGGTCTTAAGGAGAGTGACTTTTATTAACCATTAAGAG69810.28070505582737426No Hit
CTCTCTGGGTGTCCTGTTGCTGGGCTCTGTGGTCCAGCCCCTCCACAGTG54900.22075214961929301No Hit
GGGTTAGAAAGAGCCCGAGAACCCTGCCCAGGGGTTCAGGGGTCTAGTCA45070.18122585397707716No Hit
GAGTGGAGCTGTCCCAGTTTCCCCACAGCTCCCAGGCAGGGGCAGCAACC44120.17740591696180705No Hit
TCCTTAGGGTGATCCCAGGGCTTTTGTCATTCTGTCTTCTCCATTCCCAC41780.16799680894524704No Hit
AGGTTAGACAGGCCAAGGGGAGCTTCGGGCACTGGAGCCGACCCGAAGGG38620.15529049213655913No Hit
TTCCATACCTCTGTGCTTAGAAGTTCAACAGAAAAGCCTCCCCCAGAAAA35420.14242333587459668No Hit
GTCCAGGGTGTATGCGCTGCATGAGAAGTGTGTGTGTGGCCGGGGGAGGC35340.14210165696804763No Hit
AAGCAGGACTGTAGAGTTGATAGGGGACTAGAATCTCAGGTTGAGGCTTC33790.1358691281536596No Hit
GTCTGGACCCTTGAGGACATGTGGTGAGTGAGGCTGAAGCGGGCAGCCCT33420.13438136321087016No Hit
GTGCTGAGGTGATGGCCTTGTTCCTGGTGGCTCCTCAGCCTCTCAGAACG32900.13229045031830128No Hit
GTCTTAAGGAGAGTGACTTTTATTAACCATTAAGAGTTGGTTTCAGCCAG32290.1298376486558647No Hit
TCCCAAGAGTGACAAAATTCTTAAGAGAAGCTTATCTTCGGAGGCACTCG32070.12895303166285477No Hit
GTTTCATTCCATACCTCTGTGCTTAGAAGTTCAACAGAAAAGCCTCCCCC29810.11986560255284381No Hit
GAATACAATAGTAGGACAAAGTACAGGGAAAACCCTGAATTTGATTTTAG29580.11894077569651525No Hit
GTAGAGTTGATAGGGGACTAGAATCTCAGGTTGAGGCTTCTGGGTAAGGT29210.11745301075372584No Hit
ATATAGGAATCATCAAGATCTATTGTGAAAGCATAGAAGGAGGATGGGGG28860.1160456655375737No Hit
CCTATAGGCTGCACACAGCCTCTTCAGGGATCCTGCCCTCTGGGCACTAA28430.1143166414148725No Hit
GTACAGGGAAAACCCTGAATTTGATTTTAGATACCTGAGTATGGCTATGT28390.11415580196159796No Hit
GCCTATAGGTGGGGAGGGTCTCAGGGTCTCCAGTAGAGAAAGGAACCCTT28010.11262782715548993No Hit
GATAAGAAGAGGATAGCGGGATGGGAGGAGAGCAAAAATGGTTGGGAAGA27670.11126069180265642No Hit
CCTCTAGACCTTGCTTCCTTTTATCCAATAAAATTTTACTCCACAGACTT27550.11077817344283285No Hit
GTTAATATGCTGTCCGTGAGCAACATGTTGGTATCATTTTATCAGACTGG27150.10916977891008753No Hit
TTAATATGCTGTCCGTGAGCAACATGTTGGTATCATTTTATCAGACTGGC26470.1064355082044205No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT25790.10370123749875348No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)