FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n02_S10_R2_A01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n02_S10_R2_A01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2722889
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATATAATATCCAGTGTTCATTACATCATGTGCCCTCCTTAATGTC94540.3472047520115583No Hit
CTTCTTATACAGACATCAATCACTGAATTAGTGACTGATCTTCTAATCCA50760.1864196447229395No Hit
ATAATATCCAGTGTTCATTACATCATGTGCCCTCCTTAATGTCCATCACC48470.17800945980537583No Hit
ATATAATATCCAGTGTTCATTACATCATGTGCCCTCCTTAATGTCCATCA47890.17587936930223744No Hit
CCTTAATGTCCATCACCCAGTTAAGTTGGTGACTTTTTATTCCCTTGTTC39410.14473597711842093No Hit
CTTATACAGACATCAATCACTGAATTAGTGACTGATCTTCTAATCCACTA32940.12097445029892882No Hit
GATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGT28080.10312576091056228No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)