FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715388
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGAGAGGTCCAAAACAAAGAAAAAAGGATCACCACTTGCAAAGAT44160.6172874020811084No Hit
GTATATATAATTTCCTCATTCTGTTTTTGTTTTGTTTTCAAATCCAGAGG43180.6035885421617361No Hit
CTATAAAGGCAATGTGGTAGAGAGGTCCAAAACAAAGAAAAAAGGATCAC42430.5931047207948694No Hit
ATTCCAGGGTGTGCTTAAAAAGAGCAAATACTTTTCCACATTTTTCTTTG29940.4185141489653167No Hit
TTCTAGGACTCATCGTAGGCGTGACAGCCTTGGTTCTGCTGATGATAGGG28190.3940518991092945No Hit
CTCCAGGGAGCTGCTGCTGGAGCTGGGGGCGGTGGTCAGCAGGTGCTGCT22610.3160522681398067No Hit
ATATTAATCAAGACACAAAAACGGAAATTTCTATGAACATCCTTTTTCAA20830.2911706654291098No Hit
AATTTACACTGCAGATTAACAAAGACAAAGAAAAATGTGGAAAAGTATTT20220.2826438240507249No Hit
GAGTATATATAATTTCCTCATTCTGTTTTTGTTTTGTTTTCAAATCCAGA19540.27313849267809914No Hit
GTTTTATAGCTTTGGTGGTCCTGAATTTCGTCAGTGGATGTGAGCATCTC19120.26726755271265384No Hit
TAACTGTACTCTACTTTGGTTTCATATATCCAATAATTTATTCCAGGGTG18770.2623751027414494No Hit
GTTAGTGACCATCACTGTGGGCCCAGCACTGCGTTCCGGGCTACAGGCTA18660.2608374756076423No Hit
CATCTGAGGTTTCTATTTCCTGTTCCGTCATTCACCGGCTTGTGTGACCT18600.25999876989829296No Hit
CTCTACCACATTGCCTTTATAGTAATGAAAGTTGATTTCTGGTGTACTAG18240.25496653564219696No Hit
TCTCAAAGTGGTGCCCCGGGCACTCGATGCGGCCAATAGACACATTTCGT17870.2497945171012094No Hit
GTGTAAATTTATACCAAACTAGTTTATTGAAGTAATCTGATTGACTTCCT17810.2489558113918601No Hit
GTCTTATAATGGAATTTGAGAGACCCCAGCCTGGGCTTACCCCCCTGTGG17600.24602034140913742No Hit
CTTTAAACACTGTTTCAGAACAGGCCTTCGGGTTTCTATTTCTATTGCCT17560.24546120426957121No Hit
CTATAGACCAGGTAGACCTTTGAACCACATCTGAGGTTTCTATTTCCTGT17390.24308487142641477No Hit
ATTCTGAACACTTAACATTTTCCTCCAACCTGGTGTTGGTGCATGTACAC16160.22589140438475344No Hit
ATCTCAAAGTGGTGCCCCGGGCACTCGATGCGGCCAATAGACACATTTCG16030.2240742086811632No Hit
GTCCTAGAAAGAAAAAGAGAAGCTCGAGGTAGCTGCAGAAAGCAAACTCA15950.22295593440203076No Hit
GTTTCAGAACAGGCCTTCGGGTTTCTATTTCTATTGCCTTGATGGCTCCA15790.2207193858437659No Hit
GCCTAGTACACCAGAAATCAACTTTCATTACTATAAAGGCAATGTGGTAG15790.2207193858437659No Hit
CATATATCCAATAATTTATTCCAGGGTGTGCTTAAAAAGAGCAAATACTT15220.21275168160494726No Hit
ATACAAAGGGACACTCAAACCAAAGCCAGAGACAGCGAGAAGTTGCTTCT14940.20883772162798372No Hit
ATCTAAGACCACAGTGCTCAAGGGAAGTCAGAAAGGGGACTCAGACCGAT14860.20771944734885123No Hit
ATTTATTCCAGGGTGTGCTTAAAAAGAGCAAATACTTTTCCACATTTTTC14740.20604203593015258No Hit
ATCATCAGCAGAACCAAGGCTGTCACGCCTACGATGAGTCCTAGAAAGAA14720.20576246736036946No Hit
GTCATGGACTTGGCTGCTTGGGCCCTGGTGCGTTTGAGTTTGCTTTCTGC14600.2040850559416708No Hit
GTTCTGAAACAGTGTTTAAAGAACCTGCGCCCCACCGCTGCGTGGGGGCC14430.20170872309851437No Hit
CCCTAGGCCTGGACACACAGCCAAGTCCCTGCAAGCCTTTGGTCCCCACC14140.19765497883665928No Hit
ACTTAAGGCTCCGTTAAAATTAAAAGGAGAGACACTCTCCATTGTTTCAT13950.19499907742371972No Hit
GTACTCTACTTTGGTTTCATATATCCAATAATTTATTCCAGGGTGTGCTT13840.1934614502899126No Hit
CTTCTGAAGAAGGCCTAGTACACCAGAAATCAACTTTCATTACTATAAAG13800.1929023131503464No Hit
GTGCTGAGTATATATAATTTCCTCATTCTGTTTTTGTTTTGTTTTCAAAT13690.19136468601653928No Hit
GGCTATGGCACTCCATCCAAACTCCAAGGGCCAGGGAAGAGACATCAGCA13610.19024641173740683No Hit
GGCCATGGATCTCAAAGTGGTGCCCCGGGCACTCGATGCGGCCAATAGAC13330.18633245176044327No Hit
AGCGAAAGCAGGCCATGGATCTCAAAGTGGTGCCCCGGGCACTCGATGCG13040.18227870749858818No Hit
ACATGATACATCATGTCAAGCAGCTAATTATTAGCACCGAAGACCAGCTA13000.18171957035902195No Hit
CCTTTGAACCACATCTGAGGTTTCTATTTCCTGTTCCGTCATTCACCGGC12890.18018194322521483No Hit
GTGCTAGGCCCCAGGCTTCCTGTGTGTAGCTCGGAACCCTGCCCAGGGAC12510.17487014039933574No Hit
GAATTTGAGAGACCCCAGCCTGGGCTTACCCCCCTGTGGCATCCCAGACA12490.17459057182955265No Hit
AGGTATAACTTAATCTGTTTTATAGCTTTGGTGGTCCTGAATTTCGTCAG12440.17389165040509486No Hit
GGTTAGGGGAAGTAGTTGGGTTTGATGAATTTGATGAAATATTAATCAAG12300.1719346704166131No Hit
GTGGTATGGGGAAGCTGTGATCTGAGCCAGGCCTGGGGAAGTTCCTCTGG12290.17179488613172153No Hit
CCCCTGTGGCATCCCAGACATCTTCGGTCATGGACTTGGCTGCTTGGGCC12180.1702572589979144No Hit
GCTCAAGGGAAGTCAGAAAGGGGACTCAGACCGATCATCTGGGTGTCTGC12160.16997769042813132No Hit
GCCCAGGGACTGCCCACTGAGCAGTCGTTCAGAGAGGGAGCCAAGAATCT12130.16955833757345665No Hit
TCGTAGGCGTGACAGCCTTGGTTCTGCTGATGATAGGGCTGGTGAACTGT12100.169138984718782No Hit
GTATAACTTAATCTGTTTTATAGCTTTGGTGGTCCTGAATTTCGTCAGTG11860.16578416188138465No Hit
GCCCTATCATCAGCAGAACCAAGGCTGTCACGCCTACGATGAGTCCTAGA11860.16578416188138465No Hit
CTATAAAACAGATTAAGTTATACCTAAGGCAAGATTAAGTTTGGGGACTG11760.16438631903246909No Hit
GTGTCATCGTGACCCAGAAAAAGAAGAGGCCCTTCTGCCTACAAGGAGAA11600.1621497704742042No Hit
CTCCCGAACATACCTGACACACTTCTCACTTCGCTAAGAGTTTTGCCCTA11380.15907451620659No Hit
AAGCAGGGCTGCACAGGGGAGCATAGCTAATGGAAATTTTGGAGCCATTC11370.15893473192169844No Hit
AAACAATGGAGAGTGTCTCTCCTTTTAATTTTAACGGAGCCTTAAGTTGA11250.15725732050299976No Hit
GAAGGAAGCCAGGTGGGTTCCCGCCTCTCACCTGAGCCGCCGGAGCCGGC11200.156558399078542No Hit
CATTTCGTTTCGTATGAAGTTTTTGGCTTGTTGTCTTATAATGGAATTTG11170.15613904622386732No Hit
TTCCTGGGATGGCCTCCCAAGACTCCTGCCCAGCCGTGTCTGGGTGAAAC11150.15585947765408423No Hit
GTTCTGCGCCCTGGGCGCCTCGGGCGTCATCAGCCGGCAGGTGAGGCACA11120.15544012479940955No Hit
GCACAGGGGAGCATAGCTAATGGAAATTTTGGAGCCATTCTGAACACTTA10930.15278422338647No Hit
GTTTTAACTTTGGATAAATGGATTTCTCCCATTTTATTCTTCCTTTTCAG10770.1505476748282051No Hit
CGATGACACAGTTCACCAGCCCTATCATCAGCAGAACCAAGGCTGTCACG10750.150268106258422No Hit
AGCCTGGGCTTACCCCCCTGTGGCATCCCAGACATCTTCGGTCATGGACT10720.14984875340374731No Hit
GCCTTAAGTTGACTAGCTGGTCTTCGGTGCTAATAATTAGCTGCTTGACA10660.149010047694398No Hit
TCCCCGGACCCACTGGCCTTCTCGGCGCTTGGTGCCGGCGTCGGGGTCCT10640.1487304791246149No Hit
AAAGCAGGCCATGGATCTCAAAGTGGTGCCCCGGGCACTCGATGCGGCCA10630.14859069483972334No Hit
GTTCACCAGCCCTATCATCAGCAGAACCAAGGCTGTCACGCCTACGATGA10620.14845091055483178No Hit
AACTTAATCTTGCCTTAGGTATAACTTAATCTGTTTTATAGCTTTGGTGG10540.14733263627569934No Hit
GTCTGGAGCCATCAAGGCAATAGAAATAGAAACCCGAAGGCCTGTTCTGA10530.14719285199080778No Hit
ACATGAAACAATGGAGAGTGTCTCTCCTTTTAATTTTAACGGAGCCTTAA10510.1469132834210247No Hit
GGTATAACTTAATCTGTTTTATAGCTTTGGTGGTCCTGAATTTCGTCAGT10510.1469132834210247No Hit
CCTTGTAGGCAGAAGGGCCTCTTCTTTTTCTGGGTCACGATGACACAGTT10510.1469132834210247No Hit
GCCATAGCCTGTAGCCCGGAACGCAGTGCTGGGCCCACAGTGATGGTCAC10500.1467734991361331No Hit
GTGGTGCCCCGGGCACTCGATGCGGCCAATAGACACATTTCGTTTCGTAT10290.14383802915341046No Hit
GTATCATGTTACCCCTTTGTCTGGAGCCATCAAGGCAATAGAAATAGAAA10190.1424401863044949No Hit
GGTATGGGGAAGCTGTGATCTGAGCCAGGCCTGGGGAAGTTCCTCTGGCA10100.1411821277404709No Hit
GCATGTAACTGTACTCTACTTTGGTTTCATATATCCAATAATTTATTCCA10060.1406229906009047No Hit
GATTAGCAAAAATCTGAAACCCTAAAGAGATGCTCACATCCACTGACGAA9810.1371283834786158No Hit
TCTATATACTAAGTTTTAACTTTGGATAAATGGATTTCTCCCATTTTATT9680.1353111877750256No Hit
CTTCTTTTTCTGGGTCACGATGACACAGTTCACCAGCCCTATCATCAGCA9670.135171403490134No Hit
TTGTTAATCTGCAGTGTAAATTTATACCAAACTAGTTTATTGAAGTAATC9670.135171403490134No Hit
ACCCCAGCCTGGGCTTACCCCCCTGTGGCATCCCAGACATCTTCGGTCAT9630.1346122663505678No Hit
GTGTTTGAACAGTGGTATGGGGAAGCTGTGATCTGAGCCAGGCCTGGGGA9570.13377356064121845No Hit
CATCGTAGGCGTGACAGCCTTGGTTCTGCTGATGATAGGGCTGGTGAACT9550.13349399207143536No Hit
ATATTGTGCTGAGGGACATTGGGCAATGGGGAGCCATGGGGGGTGTTTGA9460.13223593350741136No Hit
CTACTTTGGTTTCATATATCCAATAATTTATTCCAGGGTGTGCTTAAAAA9460.13223593350741136No Hit
GATTACTTCAATAAACTAGTTTGGTATAAATTTACACTGCAGATTAACAA9450.1320961492225198No Hit
ATTTTATTCTTCCTTTTCAGAATTGCCCCTTGTTTTTGTTTCTTATTTTA9370.13097787494338736No Hit
GTTAATCTGCAGTGTAAATTTATACCAAACTAGTTTATTGAAGTAATCTG9280.12971981637936336No Hit
CTTTAGGGTTTCAGATTTTTGCTAATCTTTGCAAGTGGTGATCCTTTTTT9210.12874132638512248No Hit
TGGTTAGGGGAAGTAGTTGGGTTTGATGAATTTGATGAAATATTAATCAA9180.1283219735304478No Hit
TGATAGGGCTGGTGAACTGTGTCATCGTGACCCAGAAAAAGAAGAGGCCC9110.12734348353620692No Hit
TCTCAGTGCTGAGTATATATAATTTCCTCATTCTGTTTTTGTTTTGTTTT9090.1270639149664238No Hit
GCCAATAGACACATTTCGTTTCGTATGAAGTTTTTGGCTTGTTGTCTTAT9060.12664456211174915No Hit
GCTTACCTGCCGTTCAGGCAGAAAATGAGAGCCAGAATCTAAGACCACAG9020.12608542497218292No Hit
ATATATAATTTCCTCATTCTGTTTTTGTTTTGTTTTCAAATCCAGAGGTA8980.1255262878326167No Hit
GCAGGAGAGGAGGCAGCAGGGAAATCCTGCTCGCATCCCCTAGGCCTGGA8950.12510693497794204No Hit
CATGATACATCATGTCAAGCAGCTAATTATTAGCACCGAAGACCAGCTAG8940.12496715069305049No Hit
CCTACGATGAGTCCTAGAAAGAAAAAGAGAAGCTCGAGGTAGCTGCAGAA8820.12328973927435181No Hit
GAAGAAGGCCTAGTACACCAGAAATCAACTTTCATTACTATAAAGGCAAT8760.1224510335650025No Hit
AACTTAATCTGTTTTATAGCTTTGGTGGTCCTGAATTTCGTCAGTGGATG8750.12231124928011093No Hit
GTACACACCTGATTGCCAGAGTTGTTTCTTTGGATCTGCTCTCTCGGCTG8740.12217146499521937No Hit
CCTCTCACCTGAGCCGCCGGAGCCGGCCCGGGCCTCCCCGGACCCACTGG8740.12217146499521937No Hit
GTGTTTAAAGAACCTGCGCCCCACCGCTGCGTGGGGGCCAGATTTCTGGG8730.12203168071032783No Hit
GGTCAGCAGGTGCTGCTGTTCTGCGCCCTGGGCGCCTCGGGCGTCATCAG8680.12133275928587005No Hit
TCCCTGGCCCTTGGAGTTTGGATGGAGTGCCATAGCCTGTAGCCCGGAAC8660.12105319071608693No Hit
GTACAAAGACTTGGGCAAACCTCCTTGACCCTCCCTTCACAAATCCACCA8630.12063383786141227No Hit
GGGGAGCATAGCTAATGGAAATTTTGGAGCCATTCTGAACACTTAACATT8620.12049405357652072No Hit
TCTATAGACCAGGTAGACCTTTGAACCACATCTGAGGTTTCTATTTCCTG8590.12007470072184605No Hit
GCCTTTATAGTAATGAAAGTTGATTTCTGGTGTACTAGGCCTTCTTCAGA8560.11965534786717137No Hit
GTCCAAAACAAAGAAAAAAGGATCACCACTTGCAAAGATTAGCAAAAATC8540.11937577929738827No Hit
CTGCTGTTCTGCGCCCTGGGCGCCTCGGGCGTCATCAGCCGGCAGGTGAG8500.11881664215782205No Hit
CCCCGGACCCACTGGCCTTCTCGGCGCTTGGTGCCGGCGTCGGGGTCCTG8490.11867685787293049No Hit
GTACACCAGAAATCAACTTTCATTACTATAAAGGCAATGTGGTAGAGAGG8480.11853707358803894No Hit
GGCTTCTCCTTGTAGGCAGAAGGGCCTCTTCTTTTTCTGGGTCACGATGA8370.11699944645423184No Hit
ATGTGGTAGAGAGGTCCAAAACAAAGAAAAAAGGATCACCACTTGCAAAG8360.11685966216934028No Hit
GCTTGGGCCCTGGTGCGTTTGAGTTTGCTTTCTGCAGCTACCTCGAGCTT8350.11671987788444871No Hit
GGGTATGCCAGGGAAATTCTTGGCTAATTATTTCTCACTCCCGAACATAC8330.1164403093146656No Hit
CTTAAAAAGAGCAAATACTTTTCCACATTTTTCTTTGTCTTTGTTAATCT8290.11588117217509938No Hit
GTATAAATTTACACTGCAGATTAACAAAGACAAAGAAAAATGTGGAAAAG8250.11532203503553318No Hit
GTTGTCTTATAATGGAATTTGAGAGACCCCAGCCTGGGCTTACCCCCCTG8200.11462311361107538No Hit
GTTTTTGGCTTGTTGTCTTATAATGGAATTTGAGAGACCCCAGCCTGGGC8160.11406397647150918No Hit
TTCTGAACCTGTTTGCTCATCAGTGACGGGGCTGCTGATGTCTCTTCCCT8120.11350483933194294No Hit
ACTTTGGTTTCATATATCCAATAATTTATTCCAGGGTGTGCTTAAAAAGA8120.11350483933194294No Hit
GCCCAGGGTATGCCAGGGAAATTCTTGGCTAATTATTTCTCACTCCCGAA8100.11322527076215982No Hit
AATTTATTCCAGGGTGTGCTTAAAAAGAGCAAATACTTTTCCACATTTTT8080.11294570219237672No Hit
GTACAGTTACATGCATAAAATAAGAAACAAAAACAAGGGGCAATTCTGAA8060.11266613362259362No Hit
GCTCCAGACAAAGGGGTAACATGATACATCATGTCAAGCAGCTAATTATT8040.1123865650528105No Hit
CAATAATTTATTCCAGGGTGTGCTTAAAAAGAGCAAATACTTTTCCACAT7960.11126829077367806No Hit
ACCTGATACAAAGGGACACTCAAACCAAAGCCAGAGACAGCGAGAAGTTG7890.11028980077943716No Hit
TCTGCGCCCTGGGCGCCTCGGGCGTCATCAGCCGGCAGGTGAGGCACATT7880.11015001649454562No Hit
GTAGTTGGGTTTGATGAATTTGATGAAATATTAATCAAGACACAAAAACG7860.10987044792476251No Hit
TCTTTAAACACTGTTTCAGAACAGGCCTTCGGGTTTCTATTTCTATTGCC7800.10903174221541317No Hit
ATGTAACTGTACTCTACTTTGGTTTCATATATCCAATAATTTATTCCAGG7790.10889195793052163No Hit
TCCCAGACATCTTCGGTCATGGACTTGGCTGCTTGGGCCCTGGTGCGTTT7770.10861238936073851No Hit
GGTTGGTAGGGTGCGGAGGTCCACGAATATTGTGCTGAGGGACATTGGGC7720.10791346793628073No Hit
CAATAAACTAGTTTGGTATAAATTTACACTGCAGATTAACAAAGACAAAG7710.10777368365138917No Hit
CCCTAAAGAGATGCTCACATCCACTGACGAAATTCAGGACCACCAAAGCT7700.10763389936649763No Hit
TCCTTTTTCAACACCTGATACAAAGGGACACTCAAACCAAAGCCAGAGAC7690.10749411508160607No Hit
GAAAAAAGGATCACCACTTGCAAAGATTAGCAAAAATCTGAAACCCTAAA7660.1070747622269314No Hit
CTGTGGGCCCAGCACTGCGTTCCGGGCTACAGGCTATGGCACTCCATCCA7630.10665540937225675No Hit
AGCGAAAGCAGGGAAGGAAGCCAGGTGGGTTCCCGCCTCTCACCTGAGCC7600.10623605651758207No Hit
AAGTTATACCTAAGGCAAGATTAAGTTTGGGGACTGGGGAGAGATGAACC7560.10567691937801585No Hit
GGTTTCATATATCCAATAATTTATTCCAGGGTGTGCTTAAAAAGAGCAAA7550.10553713509312429No Hit
GTAGAAAACTGATATATGCCATCAAGAGAGGGTGGGGTAGGAACAGCACA7550.10553713509312429No Hit
AAAGCAGGGAGCTGCTGCTGGAGCTGGGGGCGGTGGTCAGCAGGTGCTGC7540.10539735080823275No Hit
GTCCTGGCGGGCGCCCCCCTGTCTGCGGTGCTGGCTGAGCTCTCCAGGGA7510.10497799795355807No Hit
ACAATGGAGAGTGTCTCTCCTTTTAATTTTAACGGAGCCTTAAGTTGACT7500.10483821366866651No Hit
GAAGTAATCTGATTGACTTCCTCGCCTCCAGGTTCATCTCTCCCCAGTCC7490.10469842938377497No Hit
AGTTTATTGAAGTAATCTGATTGACTTCCTCGCCTCCAGGTTCATCTCTC7490.10469842938377497No Hit
TCTCTCCCCAGTCCCCAAACTTAATCTTGCCTTAGGTATAACTTAATCTG7460.10427907652910029No Hit
GATTAAGTTATACCTAAGGCAAGATTAAGTTTGGGGACTGGGGAGAGATG7350.10274144939529319No Hit
TTTCAGAACAGGCCTTCGGGTTTCTATTTCTATTGCCTTGATGGCTCCAG7350.10274144939529319No Hit
GAATACTTGCCAGGAGAGAAAAGAGCCTGAAAACCTGCTGAAGGCTTCTG7340.10260166511040163No Hit
CGCCTACGATGAGTCCTAGAAAGAAAAAGAGAAGCTCGAGGTAGCTGCAG7310.10218231225572696No Hit
GCCAAGTCCCTGCAAGCCTTTGGTCCCCACCCAGATTTGGGGCAGAATGC7270.10162317511616073No Hit
AGGTAGACCTTTGAACCACATCTGAGGTTTCTATTTCCTGTTCCGTCATT7220.10092425369170295No Hit
GTACTAGGCCTTCTTCAGAAGCCTTCAGCAGGTTTTCAGGCTCTTTTCTC7160.10008554798235363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)