FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_H11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_H11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237364
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA80420.3594408419908428No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT77570.3467026375681382No Hit
ATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGACA70110.3133598287985326No Hit
TTATAATACTCTATCATCCACAAAGAAATGGTCAACCAAAATCTGCCTAT57100.25521104299523906No Hit
GTCATGAGAAGCATATCCTTTTTTTGTTGTTGTTGTTTTGTGTGTGTGTG55720.24904307032740317No Hit
GTATTAACTCTGAATAATAACGAGTCTACGTGGATGCCCCATCCTACCAA54850.24515456581941963No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT46760.20899594344058453No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT42830.19143062997348667No Hit
ATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGA42780.19120715270291289No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT42750.19107306634056864No Hit
TTCTTATACATGCCCTTAAGGACCATCTGAATGTGAAGACTCGAGAATTA42540.19013446180415883No Hit
CTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGG42330.189195857267749No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT39670.17730686647322474No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC37570.16792082110912665No Hit
AGATGGGACCCTGCAAAAGAAGACGATGAAGAGATCTCTGGGAAATGAAT36830.16461335750463493No Hit
GTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGA35420.15831129847445477No Hit
GATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGG35120.15697043485101217No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT34450.15397583942532372No Hit
CCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGATT34440.15393114397120897No Hit
AATATATTCTTACCAATTGCCATGGAATCTTATGAGTTAAGTCAGAGGTT32390.14476857587768463No Hit
CTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT32140.14365118952481581No Hit
ACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGA30700.13721504413229138No Hit
GGATTTATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGA30420.13596357141707832No Hit
TCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCAC30340.1356060077841603No Hit
GGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGC29660.1325667169043571No Hit
AACTAGTACAGGGCAGCATTAACTAAGAAGAGATAAAGACTGAGTCTTCA29460.13167280782206203No Hit
CTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCAT28480.12729265331881623No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA28200.12604118060360317No Hit
GATAAAGACTGAGTCTTCAAAATACTGGATTCAAAACCTTCAAAAGTCTT28120.12568361697068514No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA27590.12331475790260324No Hit
AATGGAGACTGGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGCATCTG27400.12246554427442294No Hit
GAACTCAAACTCAGTGATTATGATGGACGACTAATCCAGAATAGCATAAC27360.12228676245796392No Hit
TCCTAATCCAGGTTTTTGAAGACTTTTGAAGGTTTTGAATCCAGTATTTT26690.11929216703227548No Hit
GAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTT26440.11817478067940666No Hit
CAATAGAGAAGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATAC26160.11692330796419359No Hit
GACTTAAAGCTTGTTGATAAGGAATTAGAATTCGTTTCACCTTCAAGTCG25820.11540366252429198No Hit
GTGCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAAGAGTA25790.11526957616194772No Hit
TCCCAGCACTGGGATGCTCTTCTAGGTATTTATTTCTTCTCTCATCAAAA25620.11450975344199693No Hit
CCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAG25180.11254315346094779No Hit
AAATATATTCTTACCAATTGCCATGGAATCTTATGAGTTAAGTCAGAGGT25120.11227498073625927No Hit
CTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTG24930.11142576710807897No Hit
GTACTAGTTTTGCTTCTTTGACCAAGAGCTGACATAAGAAGGTGATGTAT24890.11124698529161996No Hit
TCTTATGTCAGCTCTTGGTCAAAGAAGCAAAACTAGTACAGGGCAGCATT24540.1096826443976036No Hit
GGATAGGAACAGTGAAGGTGAGAAAACTGAAAGAGAAACTCAAAGATACA24520.1095932534893741No Hit
TATCATGATTTGGCATTCCAACCTGAATGATGCCACATATCAGAGAACAA24440.10923568985645607No Hit
CCTTATTTCTTCTTTGTCATAAAGGATAAGTTCTCTCATCCACTTTCTGT24240.10834178077416103No Hit
ATATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACC24000.10726908987540695No Hit
GAGTTAATACAGGTCTCTGTGCCACAAAAACTTTAACCTTCTCAACTCCA23750.10615170352253814No Hit
ATCCAGAATAGCATAACAATAGAGAAGATGGTGCTTTCTGCTTTTGATGA23240.10387223536268574No Hit
GATAGGAACAGTGAAGGTGAGAAAACTGAAAGAGAAACTCAAAGATACAG23020.10288893537216118No Hit
GAATAAGGGCTAAACAAAATACATCACCTTCTTATGTCAGCTCTTGGTCA22840.1020844171980956No Hit
GTCTTTATCTCTTCTTAGTTAATGCTGCCCTGTACTAGTTTTGCTTCTTT22770.10177154901929236No Hit
GCTCTATACAATTCACCTTGATGGAGGCCCCAAAGGAGACAGACATGAAA22670.10132459447814482No Hit
ATTCTTACCAATTGCCATGGAATCTTATGAGTTAAGTCAGAGGTTAAGTA22410.10016251267116125No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)