FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_H10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_H10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181307
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACAGGTATAGTTCTTCTTTTTTTCTGCTTTGTCTCTGCAAACATT33230.15233985862604393No Hit
GTCCATATGTATTTGGCTAAATTGGACAGTGTTTTCCCAAAAGCAAATGT32110.1472053223136404No Hit
GACTTAATAGAAGGGAAGATACACAACAGGTTGCTGGAAACATAGGAAAC25150.11529784665798991No Hit
TATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACCTG23200.10635825218550163No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)