FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_G12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_G12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777296
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGAAGAGAGTGGCATTCAGAAAAGCGAGGAAACAAACCAACAGGTAAC85390.48044895166590146No Hit
GTATATGCTACTGCAAATTCCCACCATCGTATCCTTCTAGCACAAGTGGT81530.4587305659833815No Hit
CACTAGAAGAGAGTGGCATTCAGAAAAGCGAGGAAACAAACCAACAGGTA54700.3077709059155031No Hit
GTTTAATTATGACTACACACTAGAAGAGAGTGGCATTCAGAAAAGCGAGG46480.2615208721563544No Hit
GCTCTAATCTTGAATTTGAACCCATACCATAGGTTAATGCCGAATTAATG44790.2520120452642666No Hit
GTTACATTCTGTATATGCTACTGCAAATTCCCACCATCGTATCCTTCTAG41050.23096884255633274No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG40390.22725533619610913No Hit
CTTTCACACTGCTCTAATCTTGAATTTGAACCCATACCATAGGTTAATGC40320.22686147946093393No Hit
GTGTTACACAACCATAATTTTGCAATCATAAAGGTCATTCTAAGTAATCA38190.2148769816620304No Hit
GTTATATTTCCCTGTGTTACACAACCATAATTTTGCAATCATAAAGGTCA37220.20941925261745933No Hit
GTCATAAACTGTTTTCTAGATAACTTAAGCTTATTGCTCTTTAAGCCCCA35120.19760355056220236No Hit
CCATAGGTTAATGCCGAATTAATGCAAAGCAGAGTTCCATCTATTCAGAG34470.1939463094498609No Hit
TTCTTATACATGCCCTTAAGGACCATCTGAATGTGAAGACTCGAGAATTA34090.19180823003033823No Hit
CATAAAGGTCATTCTAAGTAATCAAGAAAAGACTGTTACATTCTGTATAT32640.18364976908742267No Hit
ATATTTCCCTGTGTTACACAACCATAATTTTGCAATCATAAAGGTCATTC32150.18089277194119607No Hit
CTGTATATGCTACTGCAAATTCCCACCATCGTATCCTTCTAGCACAAGTG32010.1801050584708456No Hit
ATATTACTGTTATATTTCCCTGTGTTACACAACCATAATTTTGCAATCAT31630.17796697905132292No Hit
GTACTAAAGTATCTATGGGTGGCTAGCCCCAACAGCCCAATATTACTGTT26310.14803386717800524No Hit
GTCTATAATTTTCCCATAATCTTATTATGCAAATGAAACTGATGAAAACT25580.14392650408260638No Hit
CTGTTATATTTCCCTGTGTTACACAACCATAATTTTGCAATCATAAAGGT25070.1410569764406154No Hit
GCCCAATATTACTGTTATATTTCCCTGTGTTACACAACCATAATTTTGCA24160.13593683888333738No Hit
AGCATATACAGAATGTAACAGTCTTTTCTTGATTACTTAGAATGACCTTT24150.1358805736354552No Hit
GGAATAATGAATGACAATGACTAAACAAGGAGAGACAGGAAAAAACATTC22100.12434619781960911No Hit
TTCTAGCACAAGTGGTTAAGATAAAAAGTTCTGGGGCTTAAAGAGCAATA21910.12327715810984777No Hit
ACCATAATTTTGCAATCATAAAGGTCATTCTAAGTAATCAAGAAAAGACT21550.12125160918608945No Hit
GTATTAACACTGTAAAGCGACAGATTACAGTAACTAGACCTCTCCTGGTG21550.12125160918608945No Hit
TACCATAGGTTAATGCCGAATTAATGCAAAGCAGAGTTCCATCTATTCAG19410.10921084613930375No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG18040.10150250717944563No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)