FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_G09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_G09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802405
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT51650.28656156635162466No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA33430.18547440780512706No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA32680.1813133008397114No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG32150.17837278525081765No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA29740.16500176153528204No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC28420.15767821327615048No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA27290.15140881211492424No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC23440.13004846302579054No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT20640.11451366368823877No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA19240.10674626401946288No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG18500.10264063848025279No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)