FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_G08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_G08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520977
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA73420.4827160437008581No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA59300.3898809778188625No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG50800.3339958460910323No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA44680.2937585512469945No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC44640.29349556239180474No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC40190.2642380522519407No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC37380.245763085174858No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT36540.24024031921587244No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT36360.23905686936751838No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA35860.235769508677646No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT32740.21525637797284247No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC32390.21295522548993182No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG31880.20960211758626202No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG30020.1973731358199368No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG28970.19046967837120482No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA28690.1886287563848763No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC28260.18580162619158605No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA27480.18067334351538517No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA26820.17633402740475365No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT26810.1762682801909562No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA26130.1717974696527298No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA24610.1618038931555178No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA23810.15654411605172203No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC23780.15634687441032968No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG23750.15614963276893734No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA23650.15549216063096288No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA23140.15213905272729306No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA22530.14812847268564877No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT22270.14641904512691514No Hit
AAATTACACTGGCCATTCACCAGAAAAAAAATGAACTCAAGCCCAACCAT22240.1462218034855228No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG21930.14418363985780192No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA21570.14181674016109383No Hit
CTTAACCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTACCT21510.14142225687830912No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT21480.1412250152369168No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA20820.13688569912628526No Hit
TCCCAGGACTTTGGAATCATGACCTGAGCTGAAGGCAGACACTTAACAAC19900.13083695545692012No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT19840.13044247217413543No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT19790.1301137361051482No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC19730.12971925282236352No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA19500.12820706690502223No Hit
CCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAACTATCCATACAAA19430.1277468364084401No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA19410.1276153419808452No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT19220.12636614491869372No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA18890.12419648686337795No Hit
GAAGGAAAGAGAGCAAACAGGGGGAGCAACAGGCAGAGGGAGAGGGAGAA18370.12077763174591068No Hit
CTCTGATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATC18130.11919969861477195No Hit
CATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGATATGT17880.11755601826983578No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA17650.11604383235249449No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA17260.11347969101439405No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC17120.11255923002122979No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG16770.11025807753831912No Hit
CTGATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCA16590.10907462768996506No Hit
TCCATGATGTGGCCTGAGGAAAAAGTAAGATACAGTAGATACAGAGAAAT16520.10861439719338294No Hit
ATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTC16280.10703646406224421No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA16280.10703646406224421No Hit
ATATATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAAT16180.10637899192426972No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC16130.1060502558552825No Hit
CAGTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGA16100.10585301421389015No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG16070.10565577257249781No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC15870.10434082829654887No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT15560.102302664668828No Hit
CTGTTAAGGCCCTGCAGTGGCTTTTCCAATTCAGATATTGTATTTTTTTA15450.10157944531705608No Hit
GGCCACATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGA15440.10151369810325864No Hit
GTACTAAACCGTACACTTTAGAATGATTAAGGTGTGAGTTTTGTGTTATT15420.10138220367566374No Hit
TCCCAGGACCCTGCGATCATGAACTGAGTCAAAGGCAGACACTTAACCAC15400.10125070924806884No Hit
CTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTA15270.10039599546870202No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)