FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_G02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_G02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964872
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACGCCTCGCACAAGCAAGCCAAGGTCTGCCTCGTGTCCCGCATCAG93920.4779955131937348No Hit
GTATATACCCTCTACTGTGGATGGCAGAGGGGCTTTGGGAAAACATAGAC90790.46206572234730814No Hit
CTATATGACACTCTTGTTTCATTAGCATTACAACAGATTATTAGACAAAT78900.401552874691074No Hit
GATTTAAGGTGGGTGAGAAATATTGTTTGAAACTATAAGTGAAGGCTTTC45200.23004043011453165No Hit
TTATTAGACAAATCAACAGGATGAAAATTTAAAGCAACGTTATCTAACTG42090.21421242706904065No Hit
CTCTAGAGGTGGCAGTCTGGGTTCTAACACACCCTCAGGGATTCTGATTC41460.21100611133956815No Hit
CTCCAGACCTGCTGAATCACAAACTCTAGAGGTGGCAGTCTGGGTTCTAA34080.17344641279431944No Hit
GTTCTGGGCTGTGCCAGATCTGAAGGAGACGATCCCCAACTCTCCCAAGC31300.15929790846426636No Hit
GCTCTAGAGAGTGCGGCCGGCCCGGGACACGGGGACAGTGACGGTACCAA30870.15710947074415027No Hit
CCCTCAGGGATTCTGATTCATGCTAAAGTCTGAGAACCAGAGATCTACCT30240.1539031550146778No Hit
CTCTAGAGCAGCCGCGGCCTTCTTGTCCTTCTTCTTCTTCTTCTCCTTCT30150.15344510991046745No Hit
CTATATCACCGTGAATCTTGTTAGAAACGCAGACTCTCAAGCCCCACTCC29480.15003521857912375No Hit
GGCCACGACAGCGACGAGTCGGGGAGCTCCTTCTTTTGGAACCAGCTGCT28580.14545476753702022No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT27890.14194308840474085No Hit
AGCGAAAGCAGGGCCACGACAGCGACGAGTCGGGGAGCTCCTTCTTTTGG26590.13532688134392468No Hit
GCTATATCACCGTGAATCTTGTTAGAAACGCAGACTCTCAAGCCCCACTC25300.12876156818357634No Hit
ATATAAAACAGTGGGATTAGGGCTCAGAGTATAGGAAGTTTCTCCCTTCC25070.12759100847281657No Hit
ATATGACACTCTTGTTTCATTAGCATTACAACAGATTATTAGACAAATCA24450.12443558664381191No Hit
GGCATTGGCTTCGAGGCCTCTGTTGAGTCTCAGATGATGAGAGCCAACCT23760.12092390751153256No Hit
TATCTGGCCTGGGCACGAGATTTTTAAATCTTCCCTGATGATTTAAGGTG23670.12046586240732221No Hit
AAAGAGGACACTCCGTCATCCATGTAAATCGTCTGCTCAGTCTCCACGAA23280.11848100028907735No Hit
CTATAAGTGAAGGCTTTCTCAGTTAGATAACGTTGCTTTAAATTTTCATC22690.11547826016147618No Hit
GTATAGGAAGTTTCTCCCTTCCGAGTCATTCTTTAATCTCATTCCAACAT21970.11181389932779337No Hit
GTATAACGGACCCCAGGTATCCGGCCGCCAGAATCAACATGGCTGTCATG21970.11181389932779337No Hit
GTCTCAGATGATGAGAGCCAACCTGAAGCCCTGGCTGCTCCCAAAACAGC21600.10993082501048414No Hit
GGCTTCGAGGCCTCTGTTGAGTCTCAGATGATGAGAGCCAACCTGAAGCC21390.10886205310066No Hit
CTGTTGAGTCTCAGATGATGAGAGCCAACCTGAAGCCCTGGCTGCTCCCA21320.10850579579738527No Hit
GTATGATACCGCATCGGTTCCTGACAACACTAGGACCTCACCCAACAATG20440.1040271325562174No Hit
GTCTGTACGTGACCATATCTTTTCCCAGCAGCATATCTCCAAAGTTAAGG20100.10229673994031163No Hit
GGACAAAAGAGGACACTCCGTCATCCATGTAAATCGTCTGCTCAGTCTCC19660.10005740831972769No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)