FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_E09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_E09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84048
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTAC4890.5818103940605368No Hit
GTGTCATAGTTTGTCAACCATGATCTAAGAAATGTACTCTCCAACCCAAT4720.5615838568437084No Hit
GTAAGAAGGCCAAGGCCCCTGAAAGGCCAGGGACTCGCGTCAGTGAGCGT4700.5592042642299638No Hit
ACCCAATACTTCCCTCAGTTCCTTGACAAAAAGGCTTCTCCCTGGCTTCA4490.5342185417856463No Hit
GTCATAGTTTGTCAACCATGATCTAAGAAATGTACTCTCCAACCCAATAC4430.5270797639444127No Hit
AGCAGGGGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAG4160.4949552636588616No Hit
GATCTGGACCAAGCCAAGGAGGAGGTGTTCCGCCTACCCCAAGAGGAGGA3860.4592613744526937No Hit
TTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTACA3790.4509328003045879No Hit
GAATTACAGCAGGAGCTGAAGCTGGAGTGGGTCGAGGCCTAGGTGCTGGC3700.44022463354273744No Hit
GAATTGGAACAGGGATTGGGGCCGGAATTACAGCAGGAGCTGAAGCTGGA3130.37240624405101846No Hit
ATCTTAGGGGGAGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGTCCAT3100.36883685513040165No Hit
AGCGAAAGCAGGGGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGC3090.3676470588235294No Hit
GAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAGCCAGATCC3030.3605082809822958No Hit
GAACAAGAGGGAGGAATCGGTGAAGGAGGCAGTGGGGGAGAAGGCAAGAC2800.33314296592423376No Hit
GTATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGG2690.32005520654863884No Hit
CTCCAACCCAATACTTCCCTCAGTTCCTTGACAAAAAGGCTTCTCCCTGG2670.31767561393489435No Hit
CTAATAAACTAAACCATTGGAATTTCAAGTCTTGACTTTCACACCCAGGG2540.3022082619455549No Hit
AAAGCAGGGGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAG2530.3010184656386827No Hit
GAAATGTACTCTCCAACCCAATACTTCCCTCAGTTCCTTGACAAAAAGGC2450.29150009518370457No Hit
GATCTAAGAAATGTACTCTCCAACCCAATACTTCCCTCAGTTCCTTGACA2430.28912050256996No Hit
CTTAACAGCTGAGCCCCAGGCACCCTGGGCCTGATCTCTTTTTACTGAAG2380.28317152103559873No Hit
GTGTGTCATAGTTTGTCAACCATGATCTAAGAAATGTACTCTCCAACCCA2370.28198172472872646No Hit
CTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCCAGC2330.27722253950123743No Hit
CCCCAAGAGGAGGAGGAGGGGCCAGGGGCTGGGGATGAGGTTTCCTGTGG2260.26889396535313154No Hit
GGTGGAGGCAGTCACCGGCGCAGTAAGAAGGCCAAGGCCCCTGAAAGGCC2240.266514372739387No Hit
GTGCAGGCAGGAGGAGGGGCACAGGCAGAAGAACAAGAGGGAGGAATCGG2200.26175518751189797No Hit
AAAGCAGGCGGTGTGTCATAGTTTGTCAACCATGATCTAAGAAATGTACT2100.24985722444317532No Hit
GTCCTGGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGCGCAGGC2080.2474776318294308No Hit
AGCAGGCGGTGTGTCATAGTTTGTCAACCATGATCTAAGAAATGTACTCT1970.2343898724538359No Hit
GCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCC1900.22606129830573005No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG1890.2248715019988578No Hit
TCCCCGGACCCTGGGATCAGGACCTGAGCCAAAGGCACACACTTAACAGC1880.22368170569198553No Hit
CTCTGACACCTTGTGAATGGTTGGGGTAAATTACAGATTTTCCTGGAAAG1870.22249190938511326No Hit
CTCCTGTCACATCCTCCCATCATACCCGCAGCACTTCCACAACCCCACGC1850.22011231677136872No Hit
GGTCTGGGTCCTGTCCATCAGGATTTTCACAGACTTGGTGTTCTTCATGA1820.21654292785075197No Hit
TCTTCGTGGAGCCCGGGCTGAGACTCAAGGGGCAAACCACACTCCTGTCA1820.21654292785075197No Hit
GGCCAGGGGCTGGGGATGAGGTTTCCTGTGGGACTGGTGGAGGCAGTCAC1800.2141633352370074No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA1800.2141633352370074No Hit
GACTTGGTGTTCTTCATGAAGCTGACGTCTTCTGTCTTGTCGAAGAGCAG1730.20583476108890159No Hit
GTCTATAGGAGATCCGTCTCTGAGCTGCTGAACTGGGAGCACTGGTAGAA1710.20345516847515704No Hit
GTGAAGGAGGCAGTGGGGGAGAAGGCAAGACAGGAGCAGGAAGCATGGTT1710.20345516847515704No Hit
GGCAGGAGGAGGGGCACAGGCAGAAGAACAAGAGGGAGGAATCGGTGAAG1700.20226537216828477No Hit
AGCGAAAGCAGGCGGTGTGTCATAGTTTGTCAACCATGATCTAAGAAATG1680.19988577955454026No Hit
GTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAGCCAGATCCCC1660.19750618694079572No Hit
CTGCTGAGCAGAGAGCCAGATCCCCGGACCCTGGGATCAGGACCTGAGCC1660.19750618694079572No Hit
GTGGAGGAGAAGGTGAGCCAGCTGAAGGAGGAGGTGCGGCTACAGTATGA1650.19631639063392345No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA1640.1951265943270512No Hit
GGCAGAAGAACAAGAGGGAGGAATCGGTGAAGGAGGCAGTGGGGGAGAAG1640.1951265943270512No Hit
GGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAGCCAGATCCCCGG1630.19393679802017896No Hit
GGTTTCCTGTGGGACTGGTGGAGGCAGTCACCGGCGCAGTAAGAAGGCCA1610.1915572054064344No Hit
ACCATGATCTAAGAAATGTACTCTCCAACCCAATACTTCCCTCAGTTCCT1550.18441842756520083No Hit
GGCCAAGGCCCCTGAAAGGCCAGGGACTCGCGTCAGTGAGCGTCTTCGTG1550.18441842756520083No Hit
GGGATTGGGGCCGGAATTACAGCAGGAGCTGAAGCTGGAGTGGGTCGAGG1550.18441842756520083No Hit
AAGCAGGACATCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAGCGCCT1540.18322863125832856No Hit
TTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGCCACCTCATTCAGGA1480.176089853417095No Hit
GGCTTCTGCTGAGCAGAGAGCCAGATCCCCGGACCCTGGGATCAGGACCT1470.17490005711022272No Hit
CTCCCTGGCAGGGCTGCAGCGTGGGGTTGTGGAAGTGCTGCGGGTATGAT1470.17490005711022272No Hit
CTGTGGGACTGGTGGAGGCAGTCACCGGCGCAGTAAGAAGGCCAAGGCCC1390.1653816866552446No Hit
GCTGAGCAGAGAGCCAGATCCCCGGACCCTGGGATCAGGACCTGAGCCAA1390.1653816866552446No Hit
CAGTAAGAAGGCCAAGGCCCCTGAAAGGCCAGGGACTCGCGTCAGTGAGC1380.16419189034837237No Hit
GCAGGGGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAGC1380.16419189034837237No Hit
CTACATGGGCTTTGGGGCAGGCAGAGGCTCCCTGTCGGGATGATTTACTG1380.16419189034837237No Hit
ATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGGAG1350.16062250142775555No Hit
CCTGTCACATCCTCCCATCATACCCGCAGCACTTCCACAACCCCACGCTG1310.15586331620026653No Hit
TTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTTAGGGGGAGAAAGGCT1300.15467351989339426No Hit
CACTTAACAGCTGAGCCCCAGGCACCCTGGGCCTGATCTCTTTTTACTGA1290.153483723586522No Hit
GCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACC1270.15110413097277745No Hit
CTCATGAGCAAACCGCACATCAGAGGAATTAAAAGATGACCTGATGGAGA1250.14872453835903293No Hit
GGCTGAGACTCAAGGGGCAAACCACACTCCTGTCACATCCTCCCATCATA1250.14872453835903293No Hit
GTTCTGTTCCATGGAAACACTTTCCGTAGAGACCAGGGTTTCTGGTCCTG1240.1475347420521607No Hit
CATTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTTAGGGGGAGAAAGG1230.14634494574528842No Hit
AGTGGAAGCTGCCCGCAAGGATCTGGACCAAGCCAAGGAGGAGGTGTTCC1230.14634494574528842No Hit
GGGATGAGGTTTCCTGTGGGACTGGTGGAGGCAGTCACCGGCGCAGTAAG1200.1427755568246716No Hit
CAGGGGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAGCC1190.14158576051779936No Hit
AGCGAAAGCAGGACATCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAG1190.14158576051779936No Hit
GTACTCTCCAACCCAATACTTCCCTCAGTTCCTTGACAAAAAGGCTTCTC1190.14158576051779936No Hit
GTCTATATGGCACCAACTTTCTTCCACCATTTTCTTTAGTTTCAGTGCCT1180.1403959642109271No Hit
GTGCTGGCCGGGGAACTCTCTCCCTGGCAGGGCTGCAGCGTGGGGTTGTG1170.13920616790405482No Hit
GGAGTGGGTCGAGGCCTAGGTGCTGGCCGGGGAACTCTCTCCCTGGCAGG1160.13801637159718258No Hit
GATCAGGACCTGAGCCAAAGGCACACACTTAACAGCTGAGCCCCAGGCAC1150.13682657529031028No Hit
CCTATAGACTCATTTATTAAGGAGCAACGAAGTCAACCAACACCAGCCAT1140.13563677898343804No Hit
GTGCTGAAGGGGCCTTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGC1130.13444698267656577No Hit
AAAGCAGGCAGAAGAACAAGTGGAAGCTGCCCGCAAGGATCTGGACCAAG1120.1332571863696935No Hit
AGCCAAGGAGGAGGTGTTCCGCCTACCCCAAGAGGAGGAGGAGGGGCCAG1090.1296877974490767No Hit
GGCCAGGGACTCGCGTCAGTGAGCGTCTTCGTGGAGCCCGGGCTGAGACT1090.1296877974490767No Hit
GTGCAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGA1090.1296877974490767No Hit
GTGGAGGTCCTGGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGC1080.12849800114220444No Hit
GTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAA1070.12730820483533217No Hit
GGCACAGGCAGAAGAACAAGAGGGAGGAATCGGTGAAGGAGGCAGTGGGG1070.12730820483533217No Hit
GGTATGATGGGAGGATGTGACAGGAGTGTGGTTTGCCCCTTGAGTCTCAG1070.12730820483533217No Hit
GGGGATGAGGTTTCCTGTGGGACTGGTGGAGGCAGTCACCGGCGCAGTAA1040.1237388159147154No Hit
CCTCTGTAGCGCCCAGCCCAGCTTGGGGCCTTCTGGCACATTCCTCTCCA1020.12135922330097086No Hit
CCCTGGGCCTGATCTCTTTTTACTGAAGCCAGGGAGAAGCCTTTTTGTCA1020.12135922330097086No Hit
GAACAGGGATTGGGGCCGGAATTACAGCAGGAGCTGAAGCTGGAGTGGGT1020.12135922330097086No Hit
ATATCACACTGTATGTTAATTACACTGGAATTAAAAACAAAGATATGCAT1020.12135922330097086No Hit
GGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTACAGCAGG1010.12016942699409862No Hit
GTGGGGGAGAAGGCAAGACAGGAGCAGGAAGCATGGTTATTGGACTGGGG1010.12016942699409862No Hit
GAACAAGTGGAAGCTGCCCGCAAGGATCTGGACCAAGCCAAGGAGGAGGT1010.12016942699409862No Hit
TCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAG1000.11897963068722633No Hit
ATCATACCCGCAGCACTTCCACAACCCCACGCTGCAGCCCTGCCAGGGAG1000.11897963068722633No Hit
GCTGAGAGGGAAGAGTCCAAAAAGATGTCAGATTACGGGATCTTCCCCTT990.11778983438035409No Hit
AGGCAAGACAGGAGCAGGAAGCATGGTTATTGGACTGGGGGCCGGAATTG990.11778983438035409No Hit
GAAGGAGGCAGTGGGGGAGAAGGCAAGACAGGAGCAGGAAGCATGGTTAT990.11778983438035409No Hit
GCGAAAGCAGGGGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCA990.11778983438035409No Hit
GCAGAGAGCCAGATCCCCGGACCCTGGGATCAGGACCTGAGCCAAAGGCA990.11778983438035409No Hit
GTATAAGGCTTAGTCTCTAGATTCATTTTATTCTATTTTCTTTTAAATAT980.11660003807348182No Hit
GGGCTGGGGATGAGGTTTCCTGTGGGACTGGTGGAGGCAGTCACCGGCGC980.11660003807348182No Hit
GTTCTGCAGCGAGAACGCAGCGCAGGCGCTGCACCTCGGGGAGCGCATGC970.11541024176660956No Hit
CCACACTCCTGTCACATCCTCCCATCATACCCGCAGCACTTCCACAACCC970.11541024176660956No Hit
GCGCAGTAAGAAGGCCAAGGCCCCTGAAAGGCCAGGGACTCGCGTCAGTG960.1142204454597373No Hit
GTCACCGGCGCAGTAAGAAGGCCAAGGCCCCTGAAAGGCCAGGGACTCGC960.1142204454597373No Hit
GCGTGGGGTTGTGGAAGTGCTGCGGGTATGATGGGAGGATGTGACAGGAG960.1142204454597373No Hit
AAGCTGGAGTGGGTCGAGGCCTAGGTGCTGGCCGGGGAACTCTCTCCCTG960.1142204454597373No Hit
GGGCTGCAGCGTGGGGTTGTGGAAGTGCTGCGGGTATGATGGGAGGATGT950.11303064915286502No Hit
CCTCAGAGGCCACAATGCAGACTTTGTTCAGCCACTAAGGGCTCTGAAGC950.11303064915286502No Hit
GGCCTGCACTGAGGAAGAAGGCAGAAGACGGAGAACTGTCAGGAAACAAA950.11303064915286502No Hit
TCATGAAGCTGACGTCTTCTGTCTTGTCGAAGAGCAGCTGCAGGGAGCCC940.11184085284599277No Hit
TGGTTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATT940.11184085284599277No Hit
TTCTTGGCCACCTCATTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTT940.11184085284599277No Hit
CCTCAGTTCCTTGACAAAAAGGCTTCTCCCTGGCTTCAGTAAAAAGAGAT920.10946126023224825No Hit
ATAAAAAAGAGTGCATGTTATAAAAGAAGTCAAAGGCGGTCTTGAATAAG920.10946126023224825No Hit
CATCATACCCGCAGCACTTCCACAACCCCACGCTGCAGCCCTGCCAGGGA920.10946126023224825No Hit
GGGAGTGGGAGAGAGAGAGAAACGGGCTTCTGCTGAGCAGAGAGCCAGAT920.10946126023224825No Hit
GCATGGTTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGA920.10946126023224825No Hit
ATACTTCCCTCAGTTCCTTGACAAAAAGGCTTCTCCCTGGCTTCAGTAAA910.10827146392537598No Hit
AAAGCAGGCAGAGAGGCAGGCAGAGGCAGAGGGAGAAGCAGGCTCCCTGC910.10827146392537598No Hit
GACAAGCGCCTGGTGGAGGAGAAGGTGAGCCAGCTGAAGGAGGAGGTGCG900.1070816676185037No Hit
TGACAGGAGTGTGGTTTGCCCCTTGAGTCTCAGCCCGGGCTCCACGAAGA890.10589187131163146No Hit
ACCCCACGCTGCAGCCCTGCCAGGGAGAGAGTTCCCCGGCCAGCACCTAG890.10589187131163146No Hit
GAGAGAAACGGGCTTCTGCTGAGCAGAGAGCCAGATCCCCGGACCCTGGG880.10470207500475918No Hit
GGAGCAGGAAGCATGGTTATTGGACTGGGGGCCGGAATTGGAACAGGGAT880.10470207500475918No Hit
GATTGGGGCCGGAATTACAGCAGGAGCTGAAGCTGGAGTGGGTCGAGGCC880.10470207500475918No Hit
AAGAAGGCCAAGGCCCCTGAAAGGCCAGGGACTCGCGTCAGTGAGCGTCT880.10470207500475918No Hit
GTTTGTCAACCATGATCTAAGAAATGTACTCTCCAACCCAATACTTCCCT880.10470207500475918No Hit
GGCCCAGGGTGCCTGGGGCTCAGCTGTTAAGTGTGTGCCTTTGGCTCAGG870.10351227869788693No Hit
ATCCCAGGGCGCTGGGATCCTGACCTGAGCCGAAGGCAGCCGCTTCACCA870.10351227869788693No Hit
GAAGGAGGAGGTGCGGCTACAGTATGAAAAGCTGCACCAGCTGCTGGACG870.10351227869788693No Hit
GGACTGGTGGAGGCAGTCACCGGCGCAGTAAGAAGGCCAAGGCCCCTGAA870.10351227869788693No Hit
GGCAAGACAGGAGCAGGAAGCATGGTTATTGGACTGGGGGCCGGAATTGG860.10232248239101466No Hit
CCTGAAAGGCCAGGGACTCGCGTCAGTGAGCGTCTTCGTGGAGCCCGGGC860.10232248239101466No Hit
ACCTATGCCTGCAAGGCCATCAGCGGGAGGAAACGAGGCAGCCGGAATCC860.10232248239101466No Hit
GTCCAGAAGAGGCAATCCGGGGTGATTAGCTACTGAACCCTTTATTCCTG860.10232248239101466No Hit
CCTGTGGGACTGGTGGAGGCAGTCACCGGCGCAGTAAGAAGGCCAAGGCC850.10113268608414239No Hit
GGATGAGGTTTCCTGTGGGACTGGTGGAGGCAGTCACCGGCGCAGTAAGA850.10113268608414239No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)