FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_D12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_D12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478966
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAA104052.172388019191341No Hit
ATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATA49221.0276303537203058No Hit
ATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATG39530.8253195425145No Hit
ATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATACT38500.8038148845638313No Hit
CCTTTAAAGAGGCCAAGTAAATAACCCAGAGTCATTCACTAGGAAGTGGC38270.7990128735651383No Hit
ATACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCT36410.7601792194017947No Hit
GTTATATAGGATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTC35140.7336637673655333No Hit
AAACAATACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCAC26270.5484731692855026No Hit
ATATAGGATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAA26090.544715073721308No Hit
GTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGG24900.5198698863802441No Hit
CTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATAACCCAGAGTC24340.5081780335138611No Hit
GACAAGAAGAATCAAAGTTATATAGGATTATAAACTTTGTCTTAGCCTCA23760.4960686144736787No Hit
CTGCAGGACCGTGATCTTATGGTGCTGGGATTGAGCCCCCTCTCGGGCTT22660.47310247491471213No Hit
ACTTAGAAGTCTGGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTAT22410.46788289774221975No Hit
ATTTAGGACTTAGAAGTCTGGGAGAGAGAGAGTACAAAATACTATGTTTT21030.4390708317500616No Hit
GTATTGTTTGAAGGCCTTTTCTTCTTGAGGCTAAGACAAAGTTTATAATC19950.41652225836489437No Hit
ACTCAGTATTGTTTGAAGGCCTTTTCTTCTTGAGGCTAAGACAAAGTTTA19470.40650067019370895No Hit
ACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAA18240.38082035050504626No Hit
AAATAACCCAGAGTCATTCACTAGGAAGTGGCAGAATTATAATTAAACTC18120.37831495346224997No Hit
GGATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATA17860.37288659320285783No Hit
CTATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCACATT17420.3637001373792712No Hit
ATTATGATTTGGGCACTATTATCCTCACTTCATATTTGGGGAAACCTTTA16600.3465799242534961No Hit
GTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGTGG16110.3363495529954109No Hit
CTTTAAAGGTTTCCCCAAATATGAAGTGAGGATAATAGTGCCCAAATCAT15960.3332178066919155No Hit
GGATAATAGTGCCCAAATCATAATTATTTTTATTTTTTTTTAAGATTTTT15950.33300902360501583No Hit
AAGTTATATAGGATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCT15810.33008606038842003No Hit
ATCCTATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCAC15510.32382256778142915No Hit
ACCCAGAGTCATTCACTAGGAAGTGGCAGAATTATAATTAAACTCCATTT14510.3029442590914595No Hit
GTCTGGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAA14470.30210912674386076No Hit
ATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGTGGAATGTAAGAA14330.29918616352726496No Hit
GTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATACTGAGTAGTGGTAA14250.2975158988320674No Hit
ATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCACATTCA14130.2950105017892711No Hit
CTTCTTGTCTGTTCATTTTCTTACATTCCACATTCATATTTCCTGCTTTT14100.294384152528572No Hit
GATTTGGGCACTATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAG13150.27454975927310077No Hit
GGGCACTATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAGGCCAA12740.26598965271021324No Hit
ATCTTATGGTGCTGGGATTGAGCCCCCTCTCGGGCTTCCTGCTCAGGGGG12730.26578086962331354No Hit
GTAAATAACCCAGAGTCATTCACTAGGAAGTGGCAGAATTATAATTAAAC12570.2624403402329184No Hit
AGCGAAAGCAGGAGATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGA12120.2530451013224321No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC12100.2526275351486327No Hit
CTATGGGGGCACCTGCAGGACCGTGATCTTATGGTGCTGGGATTGAGCCC11960.2497045719320369No Hit
GGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGG11960.2497045719320369No Hit
GTTTATAATCCTATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTAC11580.24177081462984848No Hit
ATCTCATTTAATATTTAAAATAACTATGGGGGCACCTGCAGGACCGTGAT11520.24051811610845025No Hit
GGAAATATGAATGTGGAATGTAAGAAAATGAACAGACAAGAAGAATCAAA11510.24030933302155058No Hit
TAATTATGATTTGGGCACTATTATCCTCACTTCATATTTGGGGAAACCTT11460.2392654175870521No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA11380.23759515289185457No Hit
GATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATAC10390.21692562728878462No Hit
ATATGAATGTGGAATGTAAGAAAATGAACAGACAAGAAGAATCAAAGTTA9740.20335472664030435No Hit
GTAATATAGTGCTTTTCATCACCTATCAGTCCATTGTCTGCCTGTGGCCA9620.20084932959750795No Hit
AAGCAGGAGATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAGAG9570.19980541416300948No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC9500.1983439325547116No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC9430.1968824509464137No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC9420.19667366785951404No Hit
GAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCA9210.19228922303462043No Hit
ATATTACAATACAACTACAGGCACGTGAGTGGCTCAGTCGGTTAAGTGTC9170.19145409068702163No Hit
GAAAGCAGGAGATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAG9140.19082774142632253No Hit
CATTTAATATTTAAAATAACTATGGGGGCACCTGCAGGACCGTGATCTTA9130.19061895833942283No Hit
ACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCTAG9050.18894869364422526No Hit
GAGGATAATAGTGCCCAAATCATAATTATTTTTATTTTTTTTTAAGATTT8910.18602573042762952No Hit
TTGTAATATAGTGCTTTTCATCACCTATCAGTCCATTGTCTGCCTGTGGC8560.17871832238614013No Hit
TTATAATTCTGCCACTTCCTAGTGAATGACTCTGGGTTATTTACTTGGCC8470.1768392746040429No Hit
TATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATAACCCAGAGTCATTCA8420.1757953591695444No Hit
ATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAGAGAGTACAAAA8390.17516900990884532No Hit
ACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAA8370.17475144373504592No Hit
GTCATTCACTAGGAAGTGGCAGAATTATAATTAAACTCCATTTTTACCAC8270.17266361286604895No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG8140.1699494327363529No Hit
GTGCTGGGATTGAGCCCCCTCTCGGGCTTCCTGCTCAGGGGGAGTCTGCT7920.1653562048245596No Hit
GATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAGAGAGTACAAA7890.16472985556386047No Hit
TATGTACACTATCTCATTTAATATTTAAAATAACTATGGGGGCACCTGCA7790.1626420246948635No Hit
AAAGCAGGGGGAGCAGCAGGAAGAGGGAGAGGGACAAGCTGGCTTCCTGA7780.16243324160796382No Hit
CTATATTACAATACAACTACAGGCACGTGAGTGGCTCAGTCGGTTAAGTG7760.16201567543416442No Hit
CTCCTACCCTTCCCCTCCTCAAATAAATAAAAATCTTAAAAAAAAATAAA7680.16034541073896685No Hit
GTAAGAAAATGAACAGACAAGAAGAATCAAAGTTATATAGGATTATAAAC7650.15971906147826778No Hit
GAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCTAGTGA7640.15951027839136805No Hit
GGGTAGGAGGGAGAAGCAGACTCCCCCTGAGCAGGAAGCCCGAGAGGGGG7490.1563785320878726No Hit
GAATTATAATTAAACTCCATTTTTACCACTACTCAGTATTGTTTGAAGGC7440.15533461665337414No Hit
GAATCAAAGTTATATAGGATTATAAACTTTGTCTTAGCCTCAAGAAGAAA7420.15491705047957474No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC7420.15491705047957474No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT7410.15470826739267504No Hit
CTATCTCATTTAATATTTAAAATAACTATGGGGGCACCTGCAGGACCGTG7360.15366435195817657No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA7250.15136773800227993No Hit
GAGAGAGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAA7230.15095017182848053No Hit
ATTTAACAGAGAGAGGGACAGCGAGAGAGCGAACACAGGCAGGAGGAGTA7140.14907112404638326No Hit
GGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGTGGAATGT7120.14865355787258386No Hit
AATATGAAGTGAGGATAATAGTGCCCAAATCATAATTATTTTTATTTTTT7050.147192076264286No Hit
ATGTAAGAAAATGAACAGACAAGAAGAATCAAAGTTATATAGGATTATAA6980.14573059465598812No Hit
CACTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATAACCCAGAG6880.14364276378699115No Hit
GGCTAATATTGTTAGTCTCTGGTTCCTCCAAAGGTTGAAGTAGGTACCGT6780.14155493291799418No Hit
AAATATGAATGTGGAATGTAAGAAAATGAACAGACAAGAAGAATCAAAGT6760.1411373667441948No Hit
GTCCTGCAGGTGCCCCCATAGTTATTTTAAATATTAAATGAGATAGTGTA6680.13946710204899723No Hit
TTGTTGGACTGGCCAGTGGCCAAAGCCTGGGCTAGCAAAGGTACTTTTGT6670.1392583189620975No Hit
ACTCTGGGTTATTTACTTGGCCTCTTTAAAGGTTTCCCCAAATATGAAGT6650.1388407527882981No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT6520.13612657265860206No Hit
AGCGAAAGCAGGGGGAGCAGCAGGAAGAGGGAGAGGGACAAGCTGGCTTC6500.13570900648480266No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA6460.1348738741372039No Hit
GTCCTAAACCCCCATCATATTTTCATTGTTGGACTGGCCAGTGGCCAAAG6420.1340387417896051No Hit
TATTTGAGGAGGGGAAGGGTAGGAGGGAGAAGCAGACTCCCCCTGAGCAG6290.13132456165990905No Hit
GGGGAAGGGTAGGAGGGAGAAGCAGACTCCCCCTGAGCAGGAAGCCCGAG6210.12965429696471148No Hit
ACTATGGGGGCACCTGCAGGACCGTGATCTTATGGTGCTGGGATTGAGCC6190.12923673079091208No Hit
GATCATGACCTGAGCCAAAGGCAGACACTAACCGACTAGGCCACCCAGAT6060.12652255066121604No Hit
CTACTCAGTATTGTTTGAAGGCCTTTTCTTCTTGAGGCTAAGACAAAGTT6040.12610498448741664No Hit
TTCCCATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTC6040.12610498448741664No Hit
GGAAGAGGGAGAGGGACAAGCTGGCTTCCTGATGAGCAAAAGACCCCCCT6000.12526985213981787No Hit
AGTAGGTACCGTGTGGTCCTAAACCCCCATCATATTTTCATTGTTGGACT5980.12485228596601845No Hit
TAATTAAACTCCATTTTTACCACTACTCAGTATTGTTTGAAGGCCTTTTC5980.12485228596601845No Hit
CTTCTAATTGGGGGGAAAAAAAGTCTTTGTTGCTTTCATCGACAGTTTTT5940.12401715361841968No Hit
ATATATGGGTGCCTGGGCGACTCAGTCATTAAGCATCTGCCTTAGGCTTA5800.12109419040182393No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT5760.12025905805422513No Hit
AAGCAGGAGTTAGCCAGTCAACAGGGAAAGAAGTCTGGGGGATAAAAGAT5710.11921514261972666No Hit
GATTAGATGTGTCGTGAAGAATTCACCTCACCCAGAGGGAGGTCTGGCCT5670.11838001027212788No Hit
CCTATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCACAT5640.1177536610114288No Hit
GTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCTAGTGAATGACTCT5630.11754487792452908No Hit
AGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAA5600.11691852866383No Hit
GATTAATCCAACTGAGCAAAAAATGTAAAAAATTAAAAATTAAAAAAATT5490.11462191470793334No Hit
GTCCATTGTCTGCCTGTGGCCATAATGATCTCCTGGCATCTAACGCATTG5460.11399556544723424No Hit
CCACTACTCAGTATTGTTTGAAGGCCTTTTCTTCTTGAGGCTAAGACAAA5400.11274286692583607No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC5390.11253408383893639No Hit
CTAGGAAGTGGCAGAATTATAATTAAACTCCATTTTTACCACTACTCAGT5360.11190773457823727No Hit
GTGAATGACTCTGGGTTATTTACTTGGCCTCTTTAAAGGTTTCCCCAAAT5360.11190773457823727No Hit
GGGTTATTTACTTGGCCTCTTTAAAGGTTTCCCCAAATATGAAGTGAGGA5330.1112813853175382No Hit
ACACTATCTCATTTAATATTTAAAATAACTATGGGGGCACCTGCAGGACC5320.1110726022306385No Hit
GAGAGGGACAGCGAGAGAGCGAACACAGGCAGGAGGAGTAGGAGAGTGAG5300.11065503605683912No Hit
GTCTTAGGGGGAAAAAAATGTGCCTTTGGAATGTACCATTGCTCACACAC5280.11023746988303972No Hit
CTTGTATAGTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCAT5240.10940233753544092No Hit
CTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGTGGAATGTAAG5210.10877598827474183No Hit
CTTAGAAGTCTGGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTATT5160.10773207284024336No Hit
GTTTAATTATAATTCTGCCACTTCCTAGTGAATGACTCTGGGTTATTTAC5140.10731450666644396No Hit
CTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCACATTCATATTTC5110.10668815740574487No Hit
GTCACAAACTGCCCGCGTGGCTGACTTTTAGTATCCACTCCACCTGGAGT5060.10564424197124639No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA5050.1054354588843467No Hit
CACTAGGAAGTGGCAGAATTATAATTAAACTCCATTTTTACCACTACTCA5030.1050178927105473No Hit
ATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGT5030.1050178927105473No Hit
CCCTAAGACTTTATTTATTTATTTGAGAGTCAGAGAGAGAGAGAGAAACA5020.10480910962364762No Hit
GCTCAGGGGGAGTCTGCTTCTCCCTCCTACCCTTCCCCTCCTCAAATAAA5010.1046003265367479No Hit
ATTTAAAATAACTATGGGGGCACCTGCAGGACCGTGATCTTATGGTGCTG5000.10439154344984822No Hit
GTTTTGAGATCCAGGGCATCTGGGTGGCCTAGTCGGTTAGTGTCTGCCTT5000.10439154344984822No Hit
AGCAGGGGGAGCAGCAGGAAGAGGGAGAGGGACAAGCTGGCTTCCTGATG4960.10355641110224942No Hit
TTCTTACATTCCACATTCATATTTCCTGCTTTTTTTCCTTTTGTCCAAAT4930.10293006184155035No Hit
TTCATATTTCCTGCTTTTTTTCCTTTTGTCCAAATAGACCAAAACATAGT4910.10251249566775095No Hit
AAATAATTATGATTTGGGCACTATTATCCTCACTTCATATTTGGGGAAAC4880.10188614640705186No Hit
CATCTAATCTACATTGGCCTGGCCGCTGGGCAGGCCGTGCCAGGGGAGTG4870.10167736332015218No Hit
ACTTTATGTAGTTGGTTAAAATCTGTGTAGCTTTTATTAATTAAAGTGAG4840.10105101405945306No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG4830.10084223097255338No Hit
AAAATGAACAGACAAGAAGAATCAAAGTTATATAGGATTATAAACTTTGT4830.10084223097255338No Hit
AATTATAATTCTGCCACTTCCTAGTGAATGACTCTGGGTTATTTACTTGG4810.10042466479875398No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)