FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_D08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_D08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607227
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA51080.31781447175787864No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA44910.2794253705294896No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT33360.2075624662850985No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC30550.19007893720053234No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT30350.1888345579062572No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC30100.1872790837884132No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT29250.18199047178774375No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA28250.17576857531636786No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC27210.16929780298613697No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG27120.16873783230371314No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG26900.16736901508001048No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG26090.16232927893819604No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA24450.15212536872513963No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC24420.15193871183099836No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA24210.15063211357200942No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG22020.1370061602996963No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA21790.13557512411127987No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA20950.13034873107532413No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA20760.12916657074576274No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA20000.12443792942751708No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC19810.12325576909795567No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC18940.11784271916785867No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA18410.11454511403802947No Hit
AAATTACACTGGCCATTCACCAGAAAAAAAATGAACTCAAGCCCAACCAT18320.11398514335560564No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA17160.10676774344880965No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA17080.10626999173109959No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG16750.10421676589554556No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA16700.10390567107197675No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC16440.10228797798941905No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT16410.10210132109527777No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT16090.10011031422443749No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)